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中文 EN

Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。
示例

当前解析结果

SmilChr03G003816

染色体: Chr03 坐标: 57181844-57192899 链方向: - 类型: protein_coding

基本信息

统一新 Gene IDSmilChr03G003816
名称SmilChr03G003816
描述glutamate synthase 1 [NADH], chloroplastic
染色体 / SeqIDChr03 / NC_080389.1
坐标与链方向57181844 - 57192899 / -

ID 对照

NCBI Gene IDLOC131014672
GeneID131014672
locus_tag-
NCBI NameLOC131014672
原始查询LOC131014672

查看完整 ID 对照表

功能注释

来源GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述glutamate synthase 1 [NADH], chloroplastic
产物glutamate synthase 1 [NADH]%2C chloroplastic
ArabidopsisAT5G53460 (GLT1)
NADH-dependent glutamate synthase 1 [Source:NCBI gene (formerly Entrezgene);Acc:835427]
identity=86.2; qcov=0.988; evalue=0; confidence=high
RiceOs01g0681900 (OsGLT1)
-
identity=80.3; qcov=0.991; evalue=0; confidence=high
GOGO:0005506 (iron ion binding); GO:0006537 (GO:0006537); GO:0010181 (FMN binding); GO:0016040 (glutamate synthase (NADH) activity); GO:0050660 (flavin adenine dinucleotide binding); GO:0051536 (iron-sulfur cluster binding)
KEGGsmil00250 (Alanine, aspartate and glutamate metabolism - Salvia miltiorrhiza (redroot sage)); smil00910 (Nitrogen metabolism - Salvia miltiorrhiza (redroot sage)); smil01100 (Metabolic pathways - Salvia miltiorrhiza (redroot sage)); smil01110 (Biosynthesis of secondary metabolites - Salvia miltiorrhiza (redroot sage)); smil01230 (Biosynthesis of amino acids - Salvia miltiorrhiza (redroot sage))
PfamPF00070 (Pyr_redox); PF00310 (GATase_2); PF00890 (FAD_binding_2); PF01493 (GXGXG); PF01593 (Amino_oxidase); PF01645 (Glu_synthase); PF04898 (Glu_syn_central); PF07992 (Pyr_redox_2); PF13450 (NAD_binding_8); PF14691 (Fer4_20)
InterPro-
eggNOG-
NRGeneID:131014672

打开注释模块

亚细胞定位 Top 5

Rank定位位置中文ScoreProtein ID
1 Cytosol 细胞质基质 1.00000 XP_057798695.1
2 Cytoplasm 细胞质 0.80000 XP_057798695.1
3 Chloroplast 叶绿体 0.20000 XP_057798694.1
4 Nucleus 细胞核 0.00000 XP_057798696.1
5 Chromosome 染色体 0.00000 XP_057798696.1

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置 REF ALT 类型 影响 携带材料数 材料
Chr3:57182248 G A EMS_canonical_SNV CDS_variant 1 C13
Chr3:57182362 G A EMS_canonical_SNV CDS_variant 2 B135,B28
Chr3:57182419 C T EMS_canonical_SNV CDS_variant 1 C13
Chr3:57182726 C T EMS_canonical_SNV CDS_variant 1 B64
Chr3:57182739 C T EMS_canonical_SNV CDS_variant 3 B40,C54,D3
Chr3:57182787 C T EMS_canonical_SNV CDS_variant 1 B46
Chr3:57183273 C T EMS_canonical_SNV CDS_variant 2 B117,C8
Chr3:57183316 C T EMS_canonical_SNV CDS_variant 1 B64
Chr3:57183325 G A EMS_canonical_SNV CDS_variant 1 C7
Chr3:57183621 C T EMS_canonical_SNV CDS_variant 2 B135,B28
Chr3:57183784 G A EMS_canonical_SNV CDS_variant 2 A20,B66
Chr3:57184312 G A EMS_canonical_SNV CDS_variant 1 B39

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057798694.1
2207 aa
N
1
1104
2207
GATase_2
Glu_syn_central
Glu_synthase
GXGXG
Fer4_20
Pyr_redox
Pyr_redox_2
FAD_binding_2
NAD_binding_8
Amino_oxidase
C
PF00310 GATase_2 113-540 aa PF04898 Glu_syn_central 590-878 aa PF01645 Glu_synthase 945-1313 aa PF01493 GXGXG 1375-1623 aa PF14691 Fer4_20 1719-1829 aa PF00070 Pyr_redox 1843-1915 aa PF07992 Pyr_redox_2 1843-2168 aa PF00890 FAD_binding_2 1844-1878 aa PF13450 NAD_binding_8 1846-1881 aa PF01593 Amino_oxidase 1852-1880 aa
XP_057798695.1
1890 aa
N
1
945
1890
GATase_2
Glu_syn_central
Glu_synthase
GXGXG
Fer4_20
Pyr_redox
Pyr_redox_2
FAD_binding_2
NAD_binding_8
Amino_oxidase
C
PF00310 GATase_2 8-223 aa PF04898 Glu_syn_central 273-561 aa PF01645 Glu_synthase 628-996 aa PF01493 GXGXG 1058-1306 aa PF14691 Fer4_20 1402-1512 aa PF00070 Pyr_redox 1526-1598 aa PF07992 Pyr_redox_2 1526-1851 aa PF00890 FAD_binding_2 1527-1561 aa PF13450 NAD_binding_8 1529-1564 aa PF01593 Amino_oxidase 1535-1563 aa
XP_057798696.1
1865 aa
N
1
932
1865
GATase_2
Glu_syn_central
Glu_synthase
GXGXG
Fer4_20
Pyr_redox
Pyr_redox_2
FAD_binding_2
NAD_binding_8
Amino_oxidase
C
PF00310 GATase_2 1-198 aa PF04898 Glu_syn_central 248-536 aa PF01645 Glu_synthase 603-971 aa PF01493 GXGXG 1033-1281 aa PF14691 Fer4_20 1377-1487 aa PF00070 Pyr_redox 1501-1573 aa PF07992 Pyr_redox_2 1501-1826 aa PF00890 FAD_binding_2 1502-1536 aa PF13450 NAD_binding_8 1504-1539 aa PF01593 Amino_oxidase 1510-1538 aa
Protein ID 来源 结构域编号 结构域名称 类型 起始 aa 终止 aa E-value Score 说明
XP_057798694.1 Pfam PF00310 GATase_2 Domain 113 540 8.9e-183 608.5 Glutamine amidotransferases class-II
XP_057798694.1 Pfam PF04898 Glu_syn_central Domain 590 878 1.4e-113 379.7 Glutamate synthase central domain
XP_057798694.1 Pfam PF01645 Glu_synthase Domain 945 1313 1.4e-150 502.3 Conserved region in glutamate synthase
XP_057798694.1 Pfam PF01493 GXGXG Domain 1375 1623 1.1e-107 359.7 GXGXG motif
XP_057798694.1 Pfam PF14691 Fer4_20 Domain 1719 1829 6.1e-22 78.3 Dihydroprymidine dehydrogenase domain II, 4Fe-4S cluster
XP_057798694.1 Pfam PF00070 Pyr_redox Domain 1843 1915 6.3e-08 33.7 Pyridine nucleotide-disulphide oxidoreductase
XP_057798694.1 Pfam PF07992 Pyr_redox_2 Domain 1843 2168 3.4e-27 96.4 Pyridine nucleotide-disulphide oxidoreductase
XP_057798694.1 Pfam PF00890 FAD_binding_2 Domain 1844 1878 9.2e-05 22.5 FAD binding domain
XP_057798694.1 Pfam PF13450 NAD_binding_8 Domain 1846 1881 3e-10 40.9 NAD(P)-binding Rossmann-like domain
XP_057798694.1 Pfam PF01593 Amino_oxidase Domain 1852 1880 1.2e-06 28.8 Flavin containing amine oxidoreductase
XP_057798695.1 Pfam PF00310 GATase_2 Domain 8 223 4.7e-104 349.2 Glutamine amidotransferases class-II
XP_057798695.1 Pfam PF04898 Glu_syn_central Domain 273 561 9.7e-114 380.2 Glutamate synthase central domain
XP_057798695.1 Pfam PF01645 Glu_synthase Domain 628 996 1.1e-150 502.7 Conserved region in glutamate synthase
XP_057798695.1 Pfam PF01493 GXGXG Domain 1058 1306 9e-108 360.0 GXGXG motif
XP_057798695.1 Pfam PF14691 Fer4_20 Domain 1402 1512 4.8e-22 78.6 Dihydroprymidine dehydrogenase domain II, 4Fe-4S cluster
XP_057798695.1 Pfam PF00070 Pyr_redox Domain 1526 1598 4e-08 34.4 Pyridine nucleotide-disulphide oxidoreductase
XP_057798695.1 Pfam PF07992 Pyr_redox_2 Domain 1526 1851 2.4e-27 96.8 Pyridine nucleotide-disulphide oxidoreductase
XP_057798695.1 Pfam PF00890 FAD_binding_2 Domain 1527 1561 6.8e-05 22.9 FAD binding domain
XP_057798695.1 Pfam PF13450 NAD_binding_8 Domain 1529 1564 2.5e-10 41.2 NAD(P)-binding Rossmann-like domain
XP_057798695.1 Pfam PF01593 Amino_oxidase Domain 1535 1563 9.2e-07 29.2 Flavin containing amine oxidoreductase
XP_057798696.1 Pfam PF00310 GATase_2 Domain 1 198 4.3e-89 299.9 Glutamine amidotransferases class-II
XP_057798696.1 Pfam PF04898 Glu_syn_central Domain 248 536 9.4e-114 380.3 Glutamate synthase central domain
XP_057798696.1 Pfam PF01645 Glu_synthase Domain 603 971 1e-150 502.7 Conserved region in glutamate synthase
XP_057798696.1 Pfam PF01493 GXGXG Domain 1033 1281 8.8e-108 360.0 GXGXG motif
XP_057798696.1 Pfam PF14691 Fer4_20 Domain 1377 1487 4.7e-22 78.6 Dihydroprymidine dehydrogenase domain II, 4Fe-4S cluster
XP_057798696.1 Pfam PF00070 Pyr_redox Domain 1501 1573 3.8e-08 34.4 Pyridine nucleotide-disulphide oxidoreductase
XP_057798696.1 Pfam PF07992 Pyr_redox_2 Domain 1501 1826 2.3e-27 96.9 Pyridine nucleotide-disulphide oxidoreductase
XP_057798696.1 Pfam PF00890 FAD_binding_2 Domain 1502 1536 6.6e-05 23.0 FAD binding domain
XP_057798696.1 Pfam PF13450 NAD_binding_8 Domain 1504 1539 2.5e-10 41.2 NAD(P)-binding Rossmann-like domain
XP_057798696.1 Pfam PF01593 Amino_oxidase Domain 1510 1538 9e-07 29.2 Flavin containing amine oxidoreductase

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

3 records

下载 cDNA FASTA
XM_057942711.1
AGGCTATATCCATCCAACTCACCACCAGCTCGCGGCGGCGTTTTTCTACTGCGAGCATATCTCATCAGCCCTAGATACAGATTACACCTTCTCCTTTCAGCCTGCATATATTTCTCTCTATCTCTCACTAGATATCGAAGTTCTCGCCTTAGTCGTCAAAAATAACGACTTTGCCTCAGAGTTTCGCTTATTTATATCCCAGTTGCCTCCTCCGTTTCAGATTTGCCTTGATTGTTGTATCGATTTTGTTTTCCTCGTTG...
XM_057942712.1
GTCTACAAAGGTCAGCTGAAACCTGAACAGTTGAAGGAATATTACTATGCAGATCTTGGCAATGAAAGCATGGGATCAACCGATTAACTGAAGCAATGCAAGGTTGAGGAATGACTTCTGAGGCATTGACGTATGAATGATCTCTTAAGTTCTTCCACACCCTTGATCACTATTTGATTTTGGATGCTGGATTGGATTCTTGGGATACACTCTAGATTCTCGACAAACACATTTCCTAGCTGGGATCGAGCTCAGCCGAT...

CDS

3 records

下载 CDS FASTA
LOC131014672
ATGTCGGCGGTGTCAGGATCCGGGATTCATGTGAGAGGCGGCGGCTTGGTGAAGCCGGCCTGCGCGCCGAGCCACCAGCTGAATGTGGTGGCGGCTTTGTCCCGGAGGGTGAGGGTGTCGCGTGCATTTGCGTCGAAGCAGAGGACGGTGAACCTGGAGAACAGGTTTGTTTACGGCACGAAGCTGCGGAGCAGTGCGGAGATGGAGAGGCTGCAGCTTTGGCAGACGGCCGGGCCGGGTCGGTCGCCGAAGCTGCGGTT...
LOC131014672
ATGCTGGATTGGATTCTTGGGATACACTCTAGATTCTCGACAAACACATTTCCTAGCTGGGATCGAGCTCAGCCGATGCGTGTCCTTGGTCACAATGGAGAGATAAATACGCTTAGAGGCAATGTGAATTGGATGAGGGCACGTGAGGGCCTTTTGAAATGCAAGGAGCTTGGCCTGTCAAAGACTGAGATGAAGAAGCTTCTGCCAATTGTAGATGCCAGCTCTTCTGACTCAGGTGCATTTGATGGTGTACTCGAGCT...

Protein

3 records

下载 Protein FASTA
XP_057798694.1
MSAVSGSGIHVRGGGLVKPACAPSHQLNVVAALSRRVRVSRAFASKQRTVNLENRFVYGTKLRSSAEMERLQLWQTAGPGRSPKLRFDVKNSMSQVPEKPLGLYDPAFDKDSCGVGFVAELSGESSRKTVTDAIEMLVRMTHRGACGCETNTGDGAGILVGLPHEFYREAAKDAGFELPPPGEYAVGMFFLPISDSRREQSKIVFTKVAESLGHSVLGWRLVPTNNSGLGKSALQTEPVIEQVFLTATPRSKADFEQQMY...
XP_057798695.1
MLDWILGIHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMRAREGLLKCKELGLSKTEMKKLLPIVDASSSDSGAFDGVLELLVRAGRSLPEAMMMMIPEAWQNDKNIDPDRRALYEYFSALMEPWDGPALISFTDGHYLGATLDRNGLRPGRFYVTHSGRVIMASEVGVVDIPPEDVARKGRLNPGMMLLVDFEKHVVVDDEALKQQYSLARPYGEWLKRQKLQLKDIVESVPESDRTPPTIAGVLPASSDDED...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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