Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr05G001145`

染色体: Chr05 坐标: 9586283-9593541 链方向: + 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr05G001145`
名称	SmilChr05G001145
描述	leucine--tRNA ligase, chloroplastic/mitochondrial
染色体 / SeqID	Chr05 / NC_080391.1
坐标与链方向	9586283 - 9593541 / `+`

ID 对照

NCBI Gene ID	`LOC130985843`
GeneID	`130985843`
locus_tag	`-`
NCBI Name	`LOC130985843`
原始查询	`SmilChr05G001145`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	leucine--tRNA ligase, chloroplastic/mitochondrial
产物	leucine--tRNA ligase%2C chloroplastic/mitochondrial
Arabidopsis	`AT4G04350` (EMB2369) tRNA synthetase class I (I, L, M and V) family protein [Source:NCBI gene (formerly Entrezgene);Acc:825755] identity=76; qcov=0.898; evalue=0; confidence=high
Rice	`Os01g0120300` - identity=74; qcov=0.885; evalue=0; confidence=high
GO	GO:0002161 (aminoacyl-tRNA deacylase activity); GO:0004823 (leucine-tRNA ligase activity); GO:0005524 (ATP binding); GO:0005829 (cytosol); GO:0006429 (leucyl-tRNA aminoacylation)
KEGG	smil00970 (Aminoacyl-tRNA biosynthesis - Salvia miltiorrhiza (redroot sage))
Pfam	PF00133 (tRNA-synt_1); PF08264 (Anticodon_1); PF09334 (tRNA-synt_1g); PF13603 (tRNA-synt_1_2)
InterPro	-
eggNOG	-
NR	GeneID:130985843

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Chloroplast	叶绿体	1.00000	`XP_057764982.1`
2	Cytoplasm	细胞质	1.00000	`XP_057764983.1`
3	Cytosol	细胞质基质	1.00000	`XP_057764983.1`
4	Chloroplast stroma	叶绿体基质	0.60000	`XP_057764982.1`
5	Nucleus	细胞核	0.00000	`XP_057764982.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr5:9586564`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	D11
`Chr5:9586713`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B89,C76
`Chr5:9588959`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B105
`Chr5:9589694`	`C`	`T`	EMS_canonical_SNV	CDS_variant	5	A19,A22,B139,C71,C79
`Chr5:9589717`	`G`	`A`	EMS_canonical_SNV	CDS_variant	5	A10,B108,B140,B76,C53
`Chr5:9591215`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	C73,D6
`Chr5:9591248`	`C`	`T`	EMS_canonical_SNV	CDS_variant	4	A21,B117,B46,C85
`Chr5:9591558`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	D11
`Chr5:9592316`	`C`	`T`	EMS_canonical_SNV	CDS_variant	5	A10,B108,B140,B76,C53
`Chr5:9592317`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C82
`Chr5:9592326`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B1,B2,C16
`Chr5:9593108`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B105

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057764982.1

967 aa

484

967

tRNA-synt_1

tRNA-synt_1g

tRNA-synt_1_2

tRNA-synt_1

Anticodon_1

PF00133 tRNA-synt_1 88-301 aa PF09334 tRNA-synt_1g 116-248 aa PF13603 tRNA-synt_1_2 297-491 aa PF00133 tRNA-synt_1 723-751 aa PF08264 Anticodon_1 808-917 aa

XP_057764983.1

843 aa

422

843

tRNA-synt_1

tRNA-synt_1_2

tRNA-synt_1

Anticodon_1

PF00133 tRNA-synt_1 62-177 aa PF13603 tRNA-synt_1_2 173-367 aa PF00133 tRNA-synt_1 379-541 aa PF00133 tRNA-synt_1 599-627 aa PF08264 Anticodon_1 684-793 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057764982.1`	Pfam	`PF00133`	tRNA-synt_1	Domain	88	301	7.8e-47	160.7	tRNA synthetases class I (I, L, M and V)
`XP_057764982.1`	Pfam	`PF09334`	tRNA-synt_1g	Family	116	248	8.1e-24	84.8	tRNA synthetases class I (M)
`XP_057764982.1`	Pfam	`PF13603`	tRNA-synt_1_2	Domain	297	491	1.8e-60	204.3	Leucyl-tRNA synthetase, editing domain
`XP_057764982.1`	Pfam	`PF00133`	tRNA-synt_1	Domain	723	751	7.8e-47	160.7	tRNA synthetases class I (I, L, M and V)
`XP_057764982.1`	Pfam	`PF08264`	Anticodon_1	Domain	808	917	6.3e-17	62.5	Anticodon-binding domain of tRNA ligase
`XP_057764983.1`	Pfam	`PF00133`	tRNA-synt_1	Domain	62	177	2.8e-24	86.4	tRNA synthetases class I (I, L, M and V)
`XP_057764983.1`	Pfam	`PF13603`	tRNA-synt_1_2	Domain	173	367	1.5e-60	204.6	Leucyl-tRNA synthetase, editing domain
`XP_057764983.1`	Pfam	`PF00133`	tRNA-synt_1	Domain	379	541	2.8e-24	86.4	tRNA synthetases class I (I, L, M and V)
`XP_057764983.1`	Pfam	`PF00133`	tRNA-synt_1	Domain	599	627	2.8e-24	86.4	tRNA synthetases class I (I, L, M and V)
`XP_057764983.1`	Pfam	`PF08264`	Anticodon_1	Domain	684	793	4.8e-17	62.9	Anticodon-binding domain of tRNA ligase

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

2 records

下载 cDNA FASTA

XM_057909000.1
ATAGAAAATGAGCCGGTAATTCTGTTTGCCGTCAAGCCCAACTAAACTTTATCTATATACTCTCTCCCTCACCACACCCTCAAATTCAATCCTCACTCAACAACGGTAACAAACTCCGGCGACGCCAATGTTCACCCAGATTCAACCCGCCGCACGGATTCACTTCGCACTACCTCCATTTCCGGCGAGTTATTCTCTATGCCGCCGCCAACCCCTTCTCCATCGCCGCGGTCCTCGCTTTTCATTTTCCCGCCTCCGAA...

XM_057908999.1
AAAATGAGCCGGTAATTCTGTTTGCCGTCAAGCCCAACTAAACTTTATCTATATACTCTCTCCCTCACCACACCCTCAAATTCAATCCTCACTCAACAACGGTAACAAACTCCGGCGACGCCAATGTTCACCCAGATTCAACCCGCCGCACGGATTCACTTCGCACTACCTCCATTTCCGGCGAGTTATTCTCTATGCCGCCGCCAACCCCTTCTCCATCGCCGCGGTCCTCGCTTTTCATTTTCCCGCCTCCGAATACT...

CDS

2 records

下载 CDS FASTA

LOC130985843
ATGTTCACCCAGATTCAACCCGCCGCACGGATTCACTTCGCACTACCTCCATTTCCGGCGAGTTATTCTCTATGCCGCCGCCAACCCCTTCTCCATCGCCGCGGTCCTCGCTTTTCATTTTCCCGCCTCCGAATACTGAATTGCTCAAAGCTCACAAATGACGGTGGAAATTCTAGTTCTAGTCAAACAAGTGAAGAACTCAGCGGCAGTGGAGAAGATGGAGTGAGGAGAGCATATCCGTTTCACGAAATAGAGCCCAA...

LOC130985843
ATGAGATCGATACTTCCAAGCCCAAATTCTACGTTCTCGACATGTTTCCTTATCCCAGTGGGGCTGGTTTACATGTGGGGCATCCTCTTGGTTATACTGCTACGGATATCCTTGCTAGACTTAAACGGATGCAAGGTTTCAATGTGTTGCATCCAATGGGTTGGGATGCATTTGGTTTACCTGCAGAGCAATATGCAATCGAGACAGGGACGCACCCAAAGATTACAACGTTCAGAAACATTGATCGATTCCGCTCTCAG...

Protein

2 records

下载 Protein FASTA

XP_057764982.1
MFTQIQPAARIHFALPPFPASYSLCRRQPLLHRRGPRFSFSRLRILNCSKLTNDGGNSSSSQTSEELSGSGEDGVRRAYPFHEIEPKWQRYWEENKTFRTPDEIDTSKPKFYVLDMFPYPSGAGLHVGHPLGYTATDILARLKRMQGFNVLHPMGWDAFGLPAEQYAIETGTHPKITTFRNIDRFRSQLKLLGFSYDWDREISTTEPDYYRWTQWIFLQLLKRGLAYQAEVPVNWCPALGTVLANEEVVDGVSERGGHPV...

XP_057764983.1
MRSILPSPNSTFSTCFLIPVGLVYMWGILLVILLRISLLDLNGCKVSMCCIQWVGMHLVYLQSNMQSRQGRTQRLQRSETLIDSALRWTQWIFLQLLKRGLAYQAEVPVNWCPALGTVLANEEVVDGVSERGGHPVIRKPMRQWMLKITAYADRLLEDLDGLDWPESVKEMQRNWIGKSEGAELQFSIMDSDGLECDVKITVYTTRPDTIFGATYLVLAPEHVLLPAIISDSQREVVEEYKELASRKSDLERTELQKEKT...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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