Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr05G001628`

染色体: Chr05 坐标: 15211923-15213264 链方向: + 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr05G001628`
名称	SmilChr05G001628
描述	probable calcium-binding protein CML15
染色体 / SeqID	Chr05 / NC_080391.1
坐标与链方向	15211923 - 15213264 / `+`

ID 对照

NCBI Gene ID	`LOC130986307`
GeneID	`130986307`
locus_tag	`-`
NCBI Name	`LOC130986307`
原始查询	`LOC130986307`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	probable calcium-binding protein CML15
产物	probable calcium-binding protein CML15
Arabidopsis	`AT1G18530` (AT1G18530) EF hand calcium-binding protein family [Source:NCBI gene (formerly Entrezgene);Acc:838434] identity=74.8; qcov=0.91; evalue=1.93e-79; confidence=high
Rice	`Os05g0577500` (OsCML14) - identity=62.9; qcov=0.958; evalue=1.07e-67; confidence=high
GO	GO:0005509 (calcium ion binding); GO:0030234 (enzyme regulator activity)
KEGG	smil04626 (Plant-pathogen interaction - Salvia miltiorrhiza (redroot sage))
Pfam	PF00036 (EF-hand_1); PF13202 (EF-hand_5); PF13405 (EF-hand_6); PF13499 (EF-hand_7); PF13833 (EF-hand_8)
InterPro	-
eggNOG	-
NR	GeneID:130986307

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Cytosol	细胞质基质	1.00000	`XP_057765666.1`
2	Cytoplasm	细胞质	0.60000	`XP_057765666.1`
3	Nucleus	细胞核	0.00000	`XP_057765666.1`
4	Chromosome	染色体	0.00000	`XP_057765666.1`
5	Centromere	着丝粒	0.00000	`XP_057765666.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr5:15212804`	`G`	`A`	EMS_canonical_SNV	CDS_variant	4	A22,B29,B96,C20
`Chr5:15212876`	`G`	`A`	EMS_canonical_SNV	CDS_variant	4	A24,B69,B74,B9
`Chr5:15212888`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C80
`Chr5:15212927`	`C`	`T`	EMS_canonical_SNV	stop_gained	8	B106,B24,B71,C1,C29,C47,C48,C80
`Chr5:15212933`	`T`	`TAA`	2bp_insertion	stop_gained,frameshift_variant	7	B106,B24,C1,C29,C47,C48,C80
`Chr5:15212957`	`C`	`T`	EMS_canonical_SNV	CDS_variant	5	B71,C1,C29,C47,C80
`Chr5:15212962`	`C`	`T`	EMS_canonical_SNV	CDS_variant	5	B71,C1,C29,C47,C80
`Chr5:15212963`	`G`	`A`	EMS_canonical_SNV	CDS_variant	4	B105,B16,C25,C30
`Chr5:15212969`	`G`	`A`	EMS_canonical_SNV	CDS_variant	3	B71,C29,C47
`Chr5:15212981`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C29
`Chr5:15213073`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B24
`Chr5:15213120`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B97

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057765666.1

166 aa

166

EF-hand_7

EF-hand_6

EF-hand_5

EF-hand_8

EF-hand_7

EF-hand_6

EF-hand_1

EF-hand_5

EF-hand_8

EF-hand_1

EF-hand_5

PF13499 EF-hand_7 12-74 aa PF13405 EF-hand_6 13-42 aa PF13202 EF-hand_5 14-37 aa PF13202 EF-hand_5 57-74 aa PF13833 EF-hand_8 63-110 aa PF13499 EF-hand_7 88-149 aa PF13405 EF-hand_6 89-113 aa PF00036 EF-hand_1 89-115 aa PF13202 EF-hand_5 92-109 aa PF13833 EF-hand_8 115-150 aa PF00036 EF-hand_1 124-151 aa PF13202 EF-hand_5 128-149 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057765666.1`	Pfam	`PF13499`	EF-hand_7	Domain	12	74	6.9e-30	103.9	EF-hand domain pair
`XP_057765666.1`	Pfam	`PF13405`	EF-hand_6	Domain	13	42	2.3e-25	86.6	EF-hand domain
`XP_057765666.1`	Pfam	`PF13202`	EF-hand_5	Domain	14	37	3.8e-23	80.6	EF hand
`XP_057765666.1`	Pfam	`PF13202`	EF-hand_5	Domain	57	74	3.8e-23	80.6	EF hand
`XP_057765666.1`	Pfam	`PF13833`	EF-hand_8	Domain	63	110	3.1e-18	66.0	EF-hand domain pair
`XP_057765666.1`	Pfam	`PF13499`	EF-hand_7	Domain	88	149	6.9e-30	103.9	EF-hand domain pair
`XP_057765666.1`	Pfam	`PF13405`	EF-hand_6	Domain	89	113	2.3e-25	86.6	EF-hand domain
`XP_057765666.1`	Pfam	`PF00036`	EF-hand_1	Domain	89	115	4e-30	101.8	EF hand domain
`XP_057765666.1`	Pfam	`PF13202`	EF-hand_5	Domain	92	109	3.8e-23	80.6	EF hand
`XP_057765666.1`	Pfam	`PF13833`	EF-hand_8	Domain	115	150	3.1e-18	66.0	EF-hand domain pair
`XP_057765666.1`	Pfam	`PF00036`	EF-hand_1	Domain	124	151	4e-30	101.8	EF hand domain
`XP_057765666.1`	Pfam	`PF13202`	EF-hand_5	Domain	128	149	3.8e-23	80.6	EF hand

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

1 records

下载 cDNA FASTA

XM_057909683.1
AGATGGAAGATCGAAACATTCTTGCTGCAGACTGCATTGTGTTGTGCTGCTGCTGCCAGTGCATGATGCTGCAGATCCTCATCTTCATACTGCTGAAGCTCCCACACAAGCTGATGAAGAAGACTAAAGAATACGCGAAGAAATTCAGAGCACGGAAGAGAGGAACGAAGATCATGCAGATAGAGATGCCAATAAATGGAGAGGACAGCTTCACAAGTCAGGGTGGATCCTTGAGAAGAGAGTCCCTTGAGTTTGGGTGC...

CDS

1 records

下载 CDS FASTA

LOC130986307
ATGGCTTCATCGCAATGCGATCCGGAGCAGCTAAAGCAGCTAAAGGAGATCTTCGACCGGTTCGACATGGACAAAGACGGCAGCCTGACGCTGCTGGAGCTGGCGGCCCTCCTCCGCTCCCTCGGCCTCAAGCCAACCGGCGACCAAATCCACACCATCTCCGCCTCCATGGACGCCAACGGCAATGGCGCCATAGAGTTTGATGAATTGGTGGAGCTCATGCTGTCCGACATTAGCGGAGAAGCCGTCCCCAACCATGA...

Protein

1 records

下载 Protein FASTA

XP_057765666.1
MASSQCDPEQLKQLKEIFDRFDMDKDGSLTLLELAALLRSLGLKPTGDQIHTISASMDANGNGAIEFDELVELMLSDISGEAVPNHDQLLEVFKAFDRDGNGFISAAELAGQMAKMGHPLTYNELTEMMQEADINGDGVISFHEFANIMAKSAVDSLSLPGSWLES

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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