当前解析结果
SmilChr05G001994| 统一新 Gene ID | SmilChr05G001994 |
|---|---|
| 名称 | SmilChr05G001994 |
| 描述 | tRNA (carboxymethyluridine(34)-5-O)-methyltransferase |
| 染色体 / SeqID | Chr05 / NC_080391.1 |
| 坐标与链方向 | 20666824 - 20669992 / + |
| NCBI Gene ID | LOC130986642 |
|---|---|
| GeneID | 130986642 |
| locus_tag | - |
| NCBI Name | LOC130986642 |
| 原始查询 | LOC130986642 |
| 来源 | GFF basic annotation; NCBI GO GAF; Pfam-A/HMMER; Arabidopsis/Rice homolog |
|---|---|
| 描述 | tRNA (carboxymethyluridine(34)-5-O)-methyltransferase |
| 产物 | tRNA (carboxymethyluridine(34)-5-O)-methyltransferase |
| Arabidopsis | AT1G36310 (AT1G36310)S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [Source:NCBI gene (formerly Entrezgene);Acc:840538] identity=62.1; qcov=0.843; evalue=2.06e-148; confidence=high |
| Rice | Os02g0750500- identity=56.3; qcov=0.863; evalue=5.52e-144; confidence=high |
| GO | GO:0000049 (tRNA binding); GO:0002098 (tRNA wobble uridine modification); GO:0005634 (nucleus); GO:0005737 (cytoplasm); GO:0008757 (S-adenosylmethionine-dependent methyltransferase activity) |
| KEGG | - |
| Pfam | PF07021 (MetW); PF08241 (Methyltransf_11); PF13489 (Methyltransf_23); PF13649 (Methyltransf_25) |
| InterPro | - |
| eggNOG | - |
| NR | GeneID:130986642 |
| Rank | 定位位置 | 中文 | Score | Protein ID |
|---|---|---|---|---|
| 1 | Cytosol | 细胞质基质 | 0.60000 | XP_057766087.1 |
| 2 | Cytoplasm | 细胞质 | 0.20000 | XP_057766087.1 |
| 3 | Mitochondrion | 线粒体 | 0.20000 | XP_057766087.1 |
| 4 | Peroxisome | 过氧化物酶体 | 0.20000 | XP_057766087.1 |
| 5 | Nucleus | 细胞核 | 0.00000 | XP_057766087.1 |
下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。
| 位置 | REF | ALT | 类型 | 影响 | 携带材料数 | 材料 |
|---|---|---|---|---|---|---|
Chr5:20667861 |
GA |
G |
1bp_deletion | frameshift_variant | 3 | B78,C23,D11 |
Chr5:20667861 |
GA |
G |
1bp_deletion | CDS_variant | 3 | B78,C23,D11 |
Chr5:20667961 |
C |
T |
EMS_canonical_SNV | CDS_variant | 3 | B12,B78,C82 |
Chr5:20667981 |
G |
A |
EMS_canonical_SNV | CDS_variant | 1 | B107 |
Chr5:20668067 |
GT |
G |
1bp_deletion | frameshift_variant | 35 | A11,A13,A14,A19,A22,A25,B125,B129,B139,B23,B37,B54,B55,B6,B66,B73,B74,B87,B93,B94,B99,C11,C19,C26,C38,C42,C50,C53,C56,C69,C73,C76,C80,D2,D8 |
Chr5:20668166 |
T |
TC |
1bp_insertion | frameshift_variant | 2 | B51,C74 |
Chr5:20668166 |
T |
TC |
1bp_insertion | CDS_variant | 2 | B51,C74 |
Chr5:20668486 |
G |
A |
EMS_canonical_SNV | stop_gained | 12 | A16,B128,B138,B36,B44,B47,B79,C1,C20,C39,C56,C71 |
Chr5:20668931 |
TC |
* |
1bp_deletion | CDS_variant | 4 | B83,C10,C52,D10 |
Chr5:20668957 |
C |
T |
EMS_canonical_SNV | CDS_variant | 2 | C28,D4 |
结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。
XP_057766087.1PF13489
Methyltransf_23 100-221 aa
PF07021
MetW 102-198 aa
PF13649
Methyltransf_25 113-200 aa
PF08241
Methyltransf_11 114-204 aa
XP_057766088.1PF13489
Methyltransf_23 100-221 aa
PF07021
MetW 102-198 aa
PF13649
Methyltransf_25 113-200 aa
PF08241
Methyltransf_11 114-204 aa
XP_057766089.1PF13489
Methyltransf_23 100-221 aa
PF07021
MetW 102-198 aa
PF13649
Methyltransf_25 113-200 aa
PF08241
Methyltransf_11 114-204 aa
XP_057766090.1PF13489
Methyltransf_23 100-221 aa
PF07021
MetW 102-198 aa
PF13649
Methyltransf_25 113-200 aa
PF08241
Methyltransf_11 114-204 aa
| Protein ID | 来源 | 结构域编号 | 结构域名称 | 类型 | 起始 aa | 终止 aa | E-value | Score | 说明 |
|---|---|---|---|---|---|---|---|---|---|
XP_057766087.1 |
Pfam | PF13489 |
Methyltransf_23 | Domain | 100 | 221 | 8e-08 | 32.9 | Methyltransferase domain |
XP_057766087.1 |
Pfam | PF07021 |
MetW | Family | 102 | 198 | 7.9e-05 | 23.1 | Methionine biosynthesis protein MetW |
XP_057766087.1 |
Pfam | PF13649 |
Methyltransf_25 | Domain | 113 | 200 | 1.4e-12 | 48.8 | Methyltransferase domain |
XP_057766087.1 |
Pfam | PF08241 |
Methyltransf_11 | Domain | 114 | 204 | 9.2e-16 | 59.0 | Methyltransferase domain |
XP_057766088.1 |
Pfam | PF13489 |
Methyltransf_23 | Domain | 100 | 221 | 8e-08 | 32.9 | Methyltransferase domain |
XP_057766088.1 |
Pfam | PF07021 |
MetW | Family | 102 | 198 | 7.9e-05 | 23.1 | Methionine biosynthesis protein MetW |
XP_057766088.1 |
Pfam | PF13649 |
Methyltransf_25 | Domain | 113 | 200 | 1.4e-12 | 48.8 | Methyltransferase domain |
XP_057766088.1 |
Pfam | PF08241 |
Methyltransf_11 | Domain | 114 | 204 | 9.2e-16 | 59.0 | Methyltransferase domain |
XP_057766089.1 |
Pfam | PF13489 |
Methyltransf_23 | Domain | 100 | 221 | 8e-08 | 32.9 | Methyltransferase domain |
XP_057766089.1 |
Pfam | PF07021 |
MetW | Family | 102 | 198 | 7.9e-05 | 23.1 | Methionine biosynthesis protein MetW |
XP_057766089.1 |
Pfam | PF13649 |
Methyltransf_25 | Domain | 113 | 200 | 1.4e-12 | 48.8 | Methyltransferase domain |
XP_057766089.1 |
Pfam | PF08241 |
Methyltransf_11 | Domain | 114 | 204 | 9.2e-16 | 59.0 | Methyltransferase domain |
XP_057766090.1 |
Pfam | PF13489 |
Methyltransf_23 | Domain | 100 | 221 | 8e-08 | 32.9 | Methyltransferase domain |
XP_057766090.1 |
Pfam | PF07021 |
MetW | Family | 102 | 198 | 7.9e-05 | 23.1 | Methionine biosynthesis protein MetW |
XP_057766090.1 |
Pfam | PF13649 |
Methyltransf_25 | Domain | 113 | 200 | 1.4e-12 | 48.8 | Methyltransferase domain |
XP_057766090.1 |
Pfam | PF08241 |
Methyltransf_11 | Domain | 114 | 204 | 9.2e-16 | 59.0 | Methyltransferase domain |
每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。
4 records
下载 cDNA FASTA4 records
下载 CDS FASTA4 records
下载 Protein FASTAupstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。