Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr05G002887`

染色体: Chr05 坐标: 34595072-34603169 链方向: + 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr05G002887`
名称	SmilChr05G002887
描述	protein disulfide isomerase-like 5-4
染色体 / SeqID	Chr05 / NC_080391.1
坐标与链方向	34595072 - 34603169 / `+`

ID 对照

NCBI Gene ID	`LOC130986960`
GeneID	`130986960`
locus_tag	`-`
NCBI Name	`LOC130986960`
原始查询	`LOC130986960`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	protein disulfide isomerase-like 5-4
产物	protein disulfide isomerase-like 5-4
Arabidopsis	`AT3G20560` (PDIL5-3) PDI-like 5-3 [Source:NCBI gene (formerly Entrezgene);Acc:821603] identity=70.7; qcov=1.01; evalue=0; confidence=high
Rice	`Os07g0524100` (PDIL5) - identity=69.8; qcov=1.01; evalue=0; confidence=high
GO	GO:0005783 (endoplasmic reticulum); GO:0030134 (COPII-coated ER to Golgi transport vesicle)
KEGG	-
Pfam	PF00085 (Thioredoxin); PF07970 (COPIIcoated_ERV); PF13850 (ERGIC_N)
InterPro	-
eggNOG	-
NR	GeneID:130986960

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Endoplasmic reticulum membrane	内质网膜	1.00000	`XP_057766493.1`
2	Endoplasmic reticulum	内质网	0.80000	`XP_057766493.1`
3	Golgi apparatus	高尔基体	0.80000	`XP_057766493.1`
4	Endoplasmic reticulum-golgi intermediate compartment	Endoplasmic reticulum-golgi intermediate compartment	0.40000	`XP_057766493.1`
5	Cis-golgi network	Cis-golgi network	0.40000	`XP_057766493.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr5:34595456`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	D6
`Chr5:34598586`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	D10
`Chr5:34598628`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	A13,B93,C4
`Chr5:34601331`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	D10
`Chr5:34602027`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	A13,C4
`Chr5:34602059`	`G`	`A`	EMS_canonical_SNV	CDS_variant	3	B127,D1,D2
`Chr5:34602490`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	D7
`Chr5:34602731`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	D10
`Chr5:34602933`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	D7

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057766493.1

480 aa

240

480

ERGIC_N

Thioredoxin

COPIIcoated_ERV

PF13850 ERGIC_N 7-97 aa PF00085 Thioredoxin 148-243 aa PF07970 COPIIcoated_ERV 285-461 aa

XP_057766494.1

480 aa

240

480

ERGIC_N

Thioredoxin

COPIIcoated_ERV

PF13850 ERGIC_N 7-97 aa PF00085 Thioredoxin 148-243 aa PF07970 COPIIcoated_ERV 285-461 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057766493.1`	Pfam	`PF13850`	ERGIC_N	Domain	7	97	2.6e-23	82.7	Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)
`XP_057766493.1`	Pfam	`PF00085`	Thioredoxin	Domain	148	243	2.6e-16	60.2	Thioredoxin
`XP_057766493.1`	Pfam	`PF07970`	COPIIcoated_ERV	Family	285	461	7.6e-28	98.6	Endoplasmic reticulum vesicle transporter
`XP_057766494.1`	Pfam	`PF13850`	ERGIC_N	Domain	7	97	2.6e-23	82.7	Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)
`XP_057766494.1`	Pfam	`PF00085`	Thioredoxin	Domain	148	243	2.6e-16	60.2	Thioredoxin
`XP_057766494.1`	Pfam	`PF07970`	COPIIcoated_ERV	Family	285	461	7.6e-28	98.6	Endoplasmic reticulum vesicle transporter

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

2 records

下载 cDNA FASTA

XM_057910511.1
ATAGTTTAGAATCAAACATGCCAATCTAATTCTAATCCAAGATTTATACGAGAAAATTTACTAATAATCGACCAATTTCTTAGCAGAAATTCACCGGGGTTCCACAATCCGTGTGATCCTGTTTTCGCTGTTACATTCAGAATCATCGCCAAGTTGCTTCATTTATTCGGAAATCGACGATTGAATAATCATGGTGTCAACCACTAAAATCAAATCCGTCGATTTTTACAGAAAAATTCCAAGAGACTTGACGGAAGCAT...

XM_057910510.1
CAAACATGCCAATCTAATTCTAATCCAAGATTTATACGAGAAAATTTACTAATAATCGACCAATTTCTTAGCAGAAATTCACCGGGGTTCCACAATCCGTGTGATCCTGTTTTCGCTGTTACATTCAGAATCATCGCCAAGTATTTTACTACTTTAATTTATTTAAAAGTTAAAACTCCACTGCACACTTCCCCCCTTCTTAACGAAAGCGAATGCGTTTGCTTTCGGTTCAGTAACACTTTGGCAGCTATACATTAACC...

CDS

2 records

下载 CDS FASTA

LOC130986960
ATGGTGTCAACCACTAAAATCAAATCCGTCGATTTTTACAGAAAAATTCCAAGAGACTTGACGGAAGCATCATTATCAGGTGCTGGATTGTCAATTCTAGCAGCCTTTTCGATGATCTTTTTATTTGGAATGGAACTGAATGATTATATGAGAGTGAGCACCTCTACATCAATTGTTGTTGACAAGAGTTCGGACGGGGACTTTCTACGAATTGATTTCAATATGAGCTTTCCAGCATTGTCTTGTGAATTTGCTTCGGT...

Protein

2 records

下载 Protein FASTA

XP_057766493.1
MVSTTKIKSVDFYRKIPRDLTEASLSGAGLSILAAFSMIFLFGMELNDYMRVSTSTSIVVDKSSDGDFLRIDFNMSFPALSCEFASVDVSDVLGTNRLNITKTIRKYSVDSRLNPTGPEFRSGPVTKAIKHDEETDEEYGEGSVLLNGLNFDRISHKHAVLVVNFYAPWCYWSNRLKPSWEKAAKIIRERYDPEIDGRILMGKVDCTEEVDLCKRNHIQGYPSIRIFRRGSDVREDHGHHEHESYYGDRDADSLVKTMED...

XP_057766494.1
MVSTTKIKSVDFYRKIPRDLTEASLSGAGLSILAAFSMIFLFGMELNDYMRVSTSTSIVVDKSSDGDFLRIDFNMSFPALSCEFASVDVSDVLGTNRLNITKTIRKYSVDSRLNPTGPEFRSGPVTKAIKHDEETDEEYGEGSVLLNGLNFDRISHKHAVLVVNFYAPWCYWSNRLKPSWEKAAKIIRERYDPEIDGRILMGKVDCTEEVDLCKRNHIQGYPSIRIFRRGSDVREDHGHHEHESYYGDRDADSLVKTMED...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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