Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr05G003644`

染色体: Chr05 坐标: 48864636-48866626 链方向: - 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr05G003644`
名称	SmilChr05G003644
描述	probable carotenoid cleavage dioxygenase 4, chloroplastic
染色体 / SeqID	Chr05 / NC_080391.1
坐标与链方向	48864636 - 48866626 / `-`

ID 对照

NCBI Gene ID	`LOC130987131`
GeneID	`130987131`
locus_tag	`-`
NCBI Name	`LOC130987131`
原始查询	`LOC130987131`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	probable carotenoid cleavage dioxygenase 4, chloroplastic
产物	probable carotenoid cleavage dioxygenase 4%2C chloroplastic
Arabidopsis	`AT4G19170` (NCED4) nine-cis-epoxycarotenoid dioxygenase 4 [Source:NCBI gene (formerly Entrezgene);Acc:827655] identity=44.1; qcov=0.996; evalue=3.69e-131; confidence=medium
Rice	`Os02g0704000` (OsNCED1) - identity=47.3; qcov=0.879; evalue=2.44e-124; confidence=high
GO	GO:0009570 (chloroplast stroma); GO:0010436 (carotenoid dioxygenase activity); GO:0016121 (carotene catabolic process)
KEGG	smil00906 (Carotenoid biosynthesis - Salvia miltiorrhiza (redroot sage)); smil01100 (Metabolic pathways - Salvia miltiorrhiza (redroot sage)); smil01110 (Biosynthesis of secondary metabolites - Salvia miltiorrhiza (redroot sage))
Pfam	PF03055 (RPE65)
InterPro	-
eggNOG	-
NR	GeneID:130987131

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Chloroplast	叶绿体	1.00000	`XP_057766736.1`
2	Chloroplast stroma	叶绿体基质	1.00000	`XP_057766736.1`
3	Nucleus	细胞核	0.00000	`XP_057766736.1`
4	Chromosome	染色体	0.00000	`XP_057766736.1`
5	Centromere	着丝粒	0.00000	`XP_057766736.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr5:48864735`	`A`	`AG`	1bp_insertion	frameshift_variant	4	B37,C1,C38,C76
`Chr5:48864735`	`A`	`AG`	1bp_insertion	CDS_variant	4	B37,C1,C38,C76
`Chr5:48864744`	`C`	`T`	EMS_canonical_SNV	CDS_variant	4	B37,C1,C38,C76
`Chr5:48864901`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	D11
`Chr5:48864982`	`C`	`T`	EMS_canonical_SNV	stop_gained	51	A10,A13,B100,B11,B111,B120,B121,B123,B126,B13,B131,B132,B138,B15,B28,B31,B35,B41,B43,B46,B47,B5,B56,B57,B61,B65,B67,B7,B70,B71,B74,B90,B96,C13,C14,C16,C24,C27,C32,C36,C4,C49,C5,C71,C75,C81,C86,C87,D10,D5,D9
`Chr5:48865006`	`A`	`AGC`	2bp_insertion	frameshift_variant	2	B74,C32
`Chr5:48865006`	`A`	`AGC`	2bp_insertion	CDS_variant	2	B74,C32
`Chr5:48865084`	`G`	`A`	EMS_canonical_SNV	CDS_variant	3	B101,B132,B68
`Chr5:48865239`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	A10,B139,D4
`Chr5:48865281`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	C1,C43,C76
`Chr5:48865296`	`G`	`A`	EMS_canonical_SNV	CDS_variant	5	B128,B26,B88,C11,C69
`Chr5:48865365`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B140,C54

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057766736.1

576 aa

288

576

RPE65

PF03055 RPE65 99-568 aa

XP_057766737.1

471 aa

236

471

RPE65

PF03055 RPE65 99-457 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057766736.1`	Pfam	`PF03055`	RPE65	Repeat	99	568	1.3e-105	355.0	Retinal pigment epithelial membrane protein
`XP_057766737.1`	Pfam	`PF03055`	RPE65	Repeat	99	457	1.8e-73	248.9	Retinal pigment epithelial membrane protein

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

2 records

下载 cDNA FASTA

XM_057910753.1
ATTGTTTAATATAGAGGCCGCACTGCTTCATAAAATTTAGAACTCATCATCTCCATCGGCATTAATTCTCTTTCCAGATTAAGATGACAACTTTAGGATTGCATTCCTCTTTCATTCCAAGAAATAGTCCTTCATCATTTAGGAAGATCAATGAGATCAACTCATTAAATAAGCCTATTAATATCATCACCGCAAAATCTTCTTCTACCAATATTTTTGAGCACCTCACAAAGAAGCTACCAAATCAAACTCCCAAATCG...

XM_057910754.1
ATGCAAATTGTTTAATATAGAGGCCGCACTGCTTCATAAAATTTAGAACTCATCATCTCCATCGGCATTAATTCTCTTTCCAGATTAAGATGACAACTTTAGGATTGCATTCCTCTTTCATTCCAAGAAATAGTCCTTCATCATTTAGGAAGATCAATGAGATCAACTCATTAAATAAGCCTATTAATATCATCACCGCAAAATCTTCTTCTACCAATATTTTTGAGCACCTCACAAAGAAGCTACCAAATCAAACTCCC...

CDS

2 records

下载 CDS FASTA

LOC130987131
ATGACAACTTTAGGATTGCATTCCTCTTTCATTCCAAGAAATAGTCCTTCATCATTTAGGAAGATCAATGAGATCAACTCATTAAATAAGCCTATTAATATCATCACCGCAAAATCTTCTTCTACCAATATTTTTGAGCACCTCACAAAGAAGCTACCAAATCAAACTCCCAAATCGCCATTAATGCCACGCATCTTCCACGCAGTAGATGAATTCATATGCGACGTTCTTGACCTCCCCCTTCCCCGCTTTCTTGACCC...

Protein

2 records

下载 Protein FASTA

XP_057766736.1
MTTLGLHSSFIPRNSPSSFRKINEINSLNKPINIITAKSSSTNIFEHLTKKLPNQTPKSPLMPRIFHAVDEFICDVLDLPLPRFLDPKYTLSGNFTPVGEIPPTACDVVEGSLPSCLHGGAYIRNGPNPHFTPKGPYHFFEGDGMLHMIKFSPKPTFCSRYVNTHKYAVEREMGYPFVPSIFSCFNGGLLASFSRFLLTAARILSGHFDPINHGFGTANANLALVSGHLLALCESDLPYAISITADGDITTLGRHHFGAS...

XP_057766737.1
MTTLGLHSSFIPRNSPSSFRKINEINSLNKPINIITAKSSSTNIFEHLTKKLPNQTPKSPLMPRIFHAVDEFICDVLDLPLPRFLDPKYTLSGNFTPVGEIPPTACDVVEGSLPSCLHGGAYIRNGPNPHFTPKGPYHFFEGDGMLHMIKFSPKPTFCSRYVNTHKYAVEREMGYPFVPSIFSCFNGGLLASFSRFLLTAARILSGHFDPINHGFGTANANLALVSGHLLALCESDLPYAISITADGDITTLGRHHFGAS...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

下载 upstream 下载 downstream 下载 upstream + gene + downstream

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