当前解析结果
SmilChr06G002460| 统一新 Gene ID | SmilChr06G002460 |
|---|---|
| 名称 | SmilChr06G002460 |
| 描述 | 24-methylenesterol C-methyltransferase 2-like |
| 染色体 / SeqID | Chr06 / NC_080392.1 |
| 坐标与链方向 | 35403494 - 35405565 / + |
| NCBI Gene ID | LOC130989261 |
|---|---|
| GeneID | 130989261 |
| locus_tag | - |
| NCBI Name | LOC130989261 |
| 原始查询 | LOC130989261 |
| 来源 | GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog |
|---|---|
| 描述 | 24-methylenesterol C-methyltransferase 2-like |
| 产物 | 24-methylenesterol C-methyltransferase 2-like |
| Arabidopsis | AT1G20330 (SMT2)sterol methyltransferase 2 [Source:NCBI gene (formerly Entrezgene);Acc:838621] identity=82.5; qcov=1; evalue=0; confidence=high |
| Rice | Os03g0136200 (Ossmt2-1)- identity=72.7; qcov=1.01; evalue=0; confidence=high |
| GO | GO:0006694 (steroid biosynthetic process); GO:0008757 (S-adenosylmethionine-dependent methyltransferase activity) |
| KEGG | smil00100 (Steroid biosynthesis - Salvia miltiorrhiza (redroot sage)); smil01100 (Metabolic pathways - Salvia miltiorrhiza (redroot sage)); smil01110 (Biosynthesis of secondary metabolites - Salvia miltiorrhiza (redroot sage)) |
| Pfam | PF01209 (Ubie_methyltran); PF02353 (CMAS); PF08241 (Methyltransf_11); PF08242 (Methyltransf_12); PF08498 (Sterol_MT_C); PF13489 (Methyltransf_23); PF13649 (Methyltransf_25); PF13847 (Methyltransf_31) |
| InterPro | - |
| eggNOG | - |
| NR | GeneID:130989261 |
| Rank | 定位位置 | 中文 | Score | Protein ID |
|---|---|---|---|---|
| 1 | Endoplasmic reticulum | 内质网 | 0.80000 | XP_057769200.1 |
| 2 | Endoplasmic reticulum membrane | 内质网膜 | 0.60000 | XP_057769200.1 |
| 3 | Mitochondrion | 线粒体 | 0.20000 | XP_057769200.1 |
| 4 | Mitochondrion membrane | 线粒体膜 | 0.20000 | XP_057769200.1 |
| 5 | Chloroplast membrane | 叶绿体膜 | 0.20000 | XP_057769200.1 |
下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。
| 位置 | REF | ALT | 类型 | 影响 | 携带材料数 | 材料 |
|---|---|---|---|---|---|---|
Chr6:35403729 |
C |
T |
EMS_canonical_SNV | CDS_variant | 3 | B138,B55,B71 |
Chr6:35403730 |
C |
T |
EMS_canonical_SNV | CDS_variant | 3 | B138,B55,B71 |
Chr6:35403740 |
C |
T |
EMS_canonical_SNV | CDS_variant | 4 | A21,B125,B2,B82 |
Chr6:35403759 |
G |
A |
EMS_canonical_SNV | stop_gained | 54 | A11,A18,A21,A25,B1,B100,B104,B113,B114,B117,B121,B122,B124,B133,B136,B20,B22,B23,B34,B35,B36,B51,B52,B54,B57,B58,B64,B67,B81,B82,B84,B88,C12,C13,C16,C20,C49,C50,C51,C54,C56,C57,C58,C59,C63,C72,C82,C87,C9,D10,D11,D3,D6,D7 |
Chr6:35403760 |
G |
A |
EMS_canonical_SNV | stop_gained | 16 | A18,A21,B114,B136,B20,B23,B51,B52,B64,B84,C20,C49,C51,C57,C59,C87 |
Chr6:35403766 |
C |
CTA |
2bp_insertion | frameshift_variant | 13 | A13,B13,B132,B33,B42,B43,B45,B62,C31,C60,C62,C75,C9 |
Chr6:35403767 |
TGC |
T |
2bp_deletion | frameshift_variant | 5 | A21,B125,B2,B82,C56 |
Chr6:35403767 |
TGC |
T |
2bp_deletion | CDS_variant | 5 | A21,B125,B2,B82,C56 |
Chr6:35403771 |
TCC |
T |
2bp_deletion | frameshift_variant | 12 | A13,B13,B132,B33,B42,B43,B45,B62,C31,C60,C62,C75 |
Chr6:35403830 |
G |
A |
EMS_canonical_SNV | CDS_variant | 1 | B80 |
Chr6:35403847 |
G |
GA |
1bp_insertion | frameshift_variant | 116 | A13,A18,A21,A22,A25,A5,A7,A8,A9,B10,B103,B106,B107,B109,B112,B114,B115,B119,B12,B120,B123,B124,B125,B13,B134,B139,B14,B15,B17,B18,B19,B2,B24,B26,B27,B28,B3,B30,B31,B35,B4,B41,B44,B47,B54,B57,B59,B6,B60,B64,B65,B66,B70,B72,B76,B78,B79,B8,B82,B84,B85,B86,B9,B91,B92,B93,B96,B99,C1,C10,C12,C14,C15,C16,C18,C19,C20,C23,C24,C28,C3,C30,C37,C38,C4,C40,C42,C43,C45,C48,C5,C50,C52,C56,C59,C6,C60,C61,C65,C69,C70,C71,C73,C77,C78,C80,C83,C86,D1,D10,D11,D3,D6,D7,D8,D9 |
Chr6:35403860 |
C |
T |
EMS_canonical_SNV | stop_gained | 63 | A11,A18,A21,A25,B1,B100,B103,B104,B107,B112,B113,B114,B117,B121,B122,B124,B131,B133,B135,B136,B22,B23,B27,B34,B35,B36,B46,B51,B52,B57,B58,B59,B61,B64,B65,B67,B74,B76,B81,B82,B86,B88,B93,C12,C13,C4,C41,C49,C50,C51,C54,C56,C59,C72,C78,C81,C82,C85,C87,C9,D10,D11,D7 |
结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。
XP_057769200.1PF02353
CMAS 75-282 aa
PF01209
Ubie_methyltran 111-233 aa
PF13489
Methyltransf_23 123-278 aa
PF13847
Methyltransf_31 125-236 aa
PF13649
Methyltransf_25 128-222 aa
PF08242
Methyltransf_12 129-224 aa
PF08241
Methyltransf_11 129-225 aa
PF08498
Sterol_MT_C 292-356 aa
| Protein ID | 来源 | 结构域编号 | 结构域名称 | 类型 | 起始 aa | 终止 aa | E-value | Score | 说明 |
|---|---|---|---|---|---|---|---|---|---|
XP_057769200.1 |
Pfam | PF02353 |
CMAS | Family | 75 | 282 | 2.6e-15 | 57.2 | Mycolic acid cyclopropane synthetase |
XP_057769200.1 |
Pfam | PF01209 |
Ubie_methyltran | Family | 111 | 233 | 1e-09 | 38.8 | ubiE/COQ5 methyltransferase family |
XP_057769200.1 |
Pfam | PF13489 |
Methyltransf_23 | Domain | 123 | 278 | 1.6e-08 | 35.1 | Methyltransferase domain |
XP_057769200.1 |
Pfam | PF13847 |
Methyltransf_31 | Domain | 125 | 236 | 3.5e-17 | 63.3 | Methyltransferase domain |
XP_057769200.1 |
Pfam | PF13649 |
Methyltransf_25 | Domain | 128 | 222 | 2.6e-20 | 73.6 | Methyltransferase domain |
XP_057769200.1 |
Pfam | PF08242 |
Methyltransf_12 | Domain | 129 | 224 | 9.5e-12 | 46.2 | Methyltransferase domain |
XP_057769200.1 |
Pfam | PF08241 |
Methyltransf_11 | Domain | 129 | 225 | 1.3e-23 | 84.1 | Methyltransferase domain |
XP_057769200.1 |
Pfam | PF08498 |
Sterol_MT_C | Family | 292 | 356 | 2e-23 | 82.9 | Sterol methyltransferase C-terminal |
每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。
1 records
下载 cDNA FASTA1 records
下载 CDS FASTA1 records
下载 Protein FASTAupstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。