Gene Card

Search by standard ID, NCBI Gene ID, GeneID, locus tag, transcript ID, protein ID, or a manually reviewed literature gene name. The page resolves the query to a Red Sage gene and integrates coordinates, sequences, annotations, subcellular localization, EMS variants, and downloads.

Resolved gene

`SmilChr06G000759`

Chromosome: Chr06 Coordinates: 7029086-7030529 Strand: - Biotype: protein_coding

Basic Information

Standard Gene ID	`SmilChr06G000759`
Name	SmilChr06G000759
Description	protein argonaute 4B-like
Chromosome / SeqID	Chr06 / NC_080392.1
Coordinates and Strand	7029086 - 7030529 / `-`

ID Mapping

NCBI Gene ID	`LOC130990421`
GeneID	`130990421`
locus_tag	`-`
NCBI Name	`LOC130990421`
Original query	`LOC130990421`

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Functional Annotation

Source	GFF basic annotation; NCBI GO GAF; Pfam-A/HMMER; Arabidopsis/Rice homolog
Description	protein argonaute 4B-like
Product	protein argonaute 4B-like
Arabidopsis	`AT2G27040` (AGO4) Argonaute family protein [Source:NCBI gene (formerly Entrezgene);Acc:817246] identity=70.7; qcov=0.984; evalue=7.4e-86; confidence=high
Rice	`Os01g0275600` (OsAGO4a) - identity=69.6; qcov=0.984; evalue=3.64e-94; confidence=high
GO	GO:0003676 (nucleic acid binding); GO:0005737 (cytoplasm); GO:0035194 (regulatory ncRNA-mediated post-transcriptional gene silencing)
KEGG	-
Pfam	PF02171 (Piwi)
InterPro	-
eggNOG	-
NR	GeneID:130990421

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Subcellular Localization Top 5

Rank	Location	Chinese	Score	Protein ID
1	Cytoplasm	细胞质	1.00000	`XP_057770639.1`
2	Nucleus	细胞核	0.40000	`XP_057770639.1`
3	Chromosome	染色体	0.00000	`XP_057770639.1`
4	Centromere	着丝粒	0.00000	`XP_057770639.1`
5	Chromatin	染色质	0.00000	`XP_057770639.1`

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EMS Mutant Variants

The table shows the first 12 candidate EMS variants for this gene. Open the EMS module for full filtering and downloads.

Position	REF	ALT	Type	Effect	Carriers	Samples
`Chr6:7029181`	`G`	`A`	EMS_canonical_SNV	CDS_variant	5	A6,B109,B131,B84,C15
`Chr6:7029239`	`C`	`T`	EMS_canonical_SNV	CDS_variant	4	B107,B49,C61,D5
`Chr6:7029241`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	A14
`Chr6:7029250`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C87
`Chr6:7029801`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B30
`Chr6:7029820`	`TC`	`T`	1bp_deletion	frameshift_variant	1	B46
`Chr6:7029820`	`TC`	`T`	1bp_deletion	CDS_variant	1	B46
`Chr6:7030045`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C56
`Chr6:7030241`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	A2,C66
`Chr6:7030292`	`C`	`T`	EMS_canonical_SNV	CDS_variant	4	B47,C13,C60,D1

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Predicted Protein Domains

Domain results are from the Pfam-A HMM database and HMMER hmmscan; positions are amino-acid coordinates on the corresponding protein sequence.

XP_057770639.1

187 aa

187

Piwi

PF02171 Piwi 12-156 aa

Protein ID	Source	Domain ID	Domain name	Type	Start aa	End aa	E-value	Score	Description
`XP_057770639.1`	Pfam	`PF02171`	Piwi	Domain	12	156	6.2e-56	190.6	Piwi domain

cDNA / CDS / Protein Sequences

Download genomic DNA (gene region only)

Every public gene has transcript sequence from the reference annotation. Protein-coding genes also have CDS and protein sequences. CDS and protein downloads do not apply to noncoding RNA. Version suffixes of original accessions are retained.

cDNA

1 records

Download cDNA FASTA

XM_057914656.1
TTCATCTTGGCATTTGAATTATTATGCTATGGCAGGGATGGAGTGAGCGAGTCCCAGTTCAACCAAATTCTAAATGCTGAGCTAGAGCAGATCATCAAGGCTTGCAAGTTTCGAGATGAGAGATGGGATCCCAAGTTCATGCTGGTTGTGGCACAAAAGAACCACCATACCAAGATTTTCCAAGCCAACTCTCCTGAAAATGTCCCTGAAGGAACCGTGATTGACAACGAAATCTGTCATCCTAGAAATAATGACTTCTA...

CDS

1 records

Download CDS FASTA

LOC130990421
TTCATCTTGGCATTTGAATTATTATGCTATGGCAGGGATGGAGTGAGCGAGTCCCAGTTCAACCAAATTCTAAATGCTGAGCTAGAGCAGATCATCAAGGCTTGCAAGTTTCGAGATGAGAGATGGGATCCCAAGTTCATGCTGGTTGTGGCACAAAAGAACCACCATACCAAGATTTTCCAAGCCAACTCTCCTGAAAATGTCCCTGAAGGAACCGTGATTGACAACGAAATCTGTCATCCTAGAAATAATGACTTCTA...

Protein

1 records

Download Protein FASTA

XP_057770639.1
FILAFELLCYGRDGVSESQFNQILNAELEQIIKACKFRDERWDPKFMLVVAQKNHHTKIFQANSPENVPEGTVIDNEICHPRNNDFYLCAHAGPIGTTRPTHYHVLYDELGYSPDDFQEFVLSLSYVYQRSTTAISIVAPIRYAHLAAAQMSQFLKFDDFSHSGGGAPAVPQFPVLCKNVRSSMFFC

Flanking Sequence Downloads

Upstream/downstream length is capped at 10000 bp. Negative-strand genes are reverse-complemented so the exported FASTA follows transcriptional 5′ to 3′ orientation.

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