Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr07G003575`

染色体: Chr07 坐标: 41734981-41749535 链方向: + 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr07G003575`
名称	SmilChr07G003575
描述	glutamine-dependent NAD(+) synthetase
染色体 / SeqID	Chr07 / NC_080393.1
坐标与链方向	41734981 - 41749535 / `+`

ID 对照

NCBI Gene ID	`LOC130992980`
GeneID	`130992980`
locus_tag	`-`
NCBI Name	`LOC130992980`
原始查询	`LOC130992980`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	glutamine-dependent NAD(+) synthetase
产物	glutamine-dependent NAD(+) synthetase
Arabidopsis	`AT1G55090` (AT1G55090) carbon-nitrogen hydrolase family protein [Source:NCBI gene (formerly Entrezgene);Acc:841952] identity=85.3; qcov=0.959; evalue=0; confidence=high
Rice	`Os07g0167100` - identity=77.6; qcov=0.716; evalue=0; confidence=high
GO	GO:0003952 (NAD+ synthase (glutamine-hydrolyzing) activity); GO:0004359 (glutaminase activity); GO:0005524 (ATP binding); GO:0005737 (cytoplasm); GO:0009435 (NAD+ biosynthetic process)
KEGG	smil00760 (Nicotinate and nicotinamide metabolism - Salvia miltiorrhiza (redroot sage)); smil01100 (Metabolic pathways - Salvia miltiorrhiza (redroot sage)); smil01240 (Biosynthesis of cofactors - Salvia miltiorrhiza (redroot sage))
Pfam	PF00795 (CN_hydrolase); PF02540 (NAD_synthase)
InterPro	-
eggNOG	-
NR	GeneID:130992980

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Cytoplasm	细胞质	1.00000	`XP_057773671.1`
2	Cytosol	细胞质基质	1.00000	`XP_057773671.1`
3	Nucleus	细胞核	0.00000	`XP_057773671.1`
4	Chromosome	染色体	0.00000	`XP_057773671.1`
5	Centromere	着丝粒	0.00000	`XP_057773671.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr7:41736198`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	D10
`Chr7:41736958`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B67
`Chr7:41737030`	`C`	`T`	EMS_canonical_SNV	stop_gained	1	C7
`Chr7:41737030`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C7
`Chr7:41737035`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C7
`Chr7:41742077`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B77
`Chr7:41742122`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B136,C60
`Chr7:41747908`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B26,B30
`Chr7:41749170`	`C`	`T`	EMS_canonical_SNV	CDS_variant	5	B120,B18,B48,C24,C27
`Chr7:41749172`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B26,B30

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057773671.1

727 aa

364

727

CN_hydrolase

NAD_synthase

PF00795 CN_hydrolase 5-281 aa PF02540 NAD_synthase 338-598 aa

XP_057773672.1

687 aa

344

687

CN_hydrolase

NAD_synthase

PF00795 CN_hydrolase 5-281 aa PF02540 NAD_synthase 338-598 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057773671.1`	Pfam	`PF00795`	CN_hydrolase	Family	5	281	7e-32	111.5	Carbon-nitrogen hydrolase
`XP_057773671.1`	Pfam	`PF02540`	NAD_synthase	Domain	338	598	2.7e-26	92.9	NAD synthase
`XP_057773672.1`	Pfam	`PF00795`	CN_hydrolase	Family	5	281	6.2e-32	111.6	Carbon-nitrogen hydrolase
`XP_057773672.1`	Pfam	`PF02540`	NAD_synthase	Domain	338	598	2e-26	93.4	NAD synthase

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

2 records

下载 cDNA FASTA

XM_057917689.1
TCCTTGTTCTTGGTCTTGGTAAAGGTCTGCTTCCACTAAAACTTCAATTCGAACCGCACGACACGTACACCAAGCGACAAGTAAACACATATTTTCCTTCCCCTCTCTCTCTACGTAGATATATCTATATATGAACAAACCTCAAGCAGACGTCGGAACCTCCAAAACCGCGAGAATACAAGTCGAATCTAGGCCATATATTCTCCGCCCACTCCTTTCTCTCTCTCTCTCCCTCTCTACTTATTCACATTACTTCTTCT...

XM_057917688.1
TCCTTGTTCTTGGTCTTGGTAAAGGTCTGCTTCCACTAAAACTTCAATTCGAACCGCACGACACGTACACCAAGCGACAAGTAAACACATATTTTCCTTCCCCTCTCTCTCTACGTAGATATATCTATATATGAACAAACCTCAAGCAGACGTCGGAACCTCCAAAACCGCGAGAATACAAGTCGAATCTAGGCCATATATTCTCCGCCCACTCCTTTCTCTCTCTCTCTCCCTCTCTACTTATTCACATTACTTCTTCT...

CDS

2 records

下载 CDS FASTA

LOC130992980
ATGAGGCTTCTGAAGGTAGCCACCTGCAACTTGAATCAATGGGCAATGGATTTCGATTGCAATATGAAGAACATTAAGGAATCCATTTCTAGGGCTAAAGAAGCCGGCGCCGTCATCCGCCTTGGTCCCGAGCTTGAAATCACCGGCTATGGCTGCGAAGACCATTTCCTGGAACTCGACACCGTCAATCATGCGTGGGATTGCTTAAAAGAATTATTAGTAGACGACTGGACTGATGGCATATTATGTAGTTTTGGTAT...

Protein

2 records

下载 Protein FASTA

XP_057773671.1
MRLLKVATCNLNQWAMDFDCNMKNIKESISRAKEAGAVIRLGPELEITGYGCEDHFLELDTVNHAWDCLKELLVDDWTDGILCSFGMPVIKGSERYNCQVLCLNRKIVMIRPKMWLANDGNYRELRWFTAWKEKGPLEDFLLPSDISEALLQTTVPFGYGYIQFLDVAIAAEVCEELFSPMPPHAELALNGVEVFLNASGSHHQLRKLDLRLRAFIGATHTRGGVYMYSNHQGCDGSRLYYDGCSCIVVNGDVVAQGSQF...

XP_057773672.1
MRLLKVATCNLNQWAMDFDCNMKNIKESISRAKEAGAVIRLGPELEITGYGCEDHFLELDTVNHAWDCLKELLVDDWTDGILCSFGMPVIKGSERYNCQVLCLNRKIVMIRPKMWLANDGNYRELRWFTAWKEKGPLEDFLLPSDISEALLQTTVPFGYGYIQFLDVAIAAEVCEELFSPMPPHAELALNGVEVFLNASGSHHQLRKLDLRLRAFIGATHTRGGVYMYSNHQGCDGSRLYYDGCSCIVVNGDVVAQGSQF...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

下载 upstream 下载 downstream 下载 upstream + gene + downstream

JBrowse

跳转到基因组浏览器