当前解析结果
SmilChr07G004221| 统一新 Gene ID | SmilChr07G004221 |
|---|---|
| 名称 | SmilChr07G004221 |
| 描述 | NADP-dependent malic enzyme-like |
| 染色体 / SeqID | Chr07 / NC_080393.1 |
| 坐标与链方向 | 54415959 - 54420353 / + |
| NCBI Gene ID | LOC130994415 |
|---|---|
| GeneID | 130994415 |
| locus_tag | - |
| NCBI Name | LOC130994415 |
| 原始查询 | LOC130994415 |
| 来源 | GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog |
|---|---|
| 描述 | NADP-dependent malic enzyme-like |
| 产物 | NADP-dependent malic enzyme-like |
| Arabidopsis | AT5G25880 (NADP-ME3)NADP-malic enzyme 3 [Source:NCBI gene (formerly Entrezgene);Acc:832657] identity=81.7; qcov=0.951; evalue=0; confidence=high |
| Rice | Os05g0186300 (OscytME2)- identity=80.9; qcov=0.951; evalue=0; confidence=high |
| GO | GO:0004471 (malate dehydrogenase (decarboxylating) (NAD+) activity); GO:0004473 (malate dehydrogenase (decarboxylating) (NADP+) activity); GO:0006090 (pyruvate metabolic process); GO:0006108 (malate metabolic process); GO:0009507 (chloroplast); GO:0051287 (NAD binding) |
| KEGG | smil00620 (Pyruvate metabolism - Salvia miltiorrhiza (redroot sage)); smil00710 (Carbon fixation by Calvin cycle - Salvia miltiorrhiza (redroot sage)); smil01100 (Metabolic pathways - Salvia miltiorrhiza (redroot sage)); smil01200 (Carbon metabolism - Salvia miltiorrhiza (redroot sage)) |
| Pfam | PF00390 (malic); PF03949 (Malic_M) |
| InterPro | - |
| eggNOG | - |
| NR | GeneID:130994415 |
| Rank | 定位位置 | 中文 | Score | Protein ID |
|---|---|---|---|---|
| 1 | Cytosol | 细胞质基质 | 1.00000 | XP_057775443.1 |
| 2 | Cytoplasm | 细胞质 | 0.80000 | XP_057775443.1 |
| 3 | Nucleus | 细胞核 | 0.00000 | XP_057775443.1 |
| 4 | Chromosome | 染色体 | 0.00000 | XP_057775443.1 |
| 5 | Centromere | 着丝粒 | 0.00000 | XP_057775443.1 |
下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。
| 位置 | REF | ALT | 类型 | 影响 | 携带材料数 | 材料 |
|---|---|---|---|---|---|---|
Chr7:54416053 |
C |
T |
EMS_canonical_SNV | CDS_variant | 2 | B136,C51 |
Chr7:54416098 |
AG |
A |
1bp_deletion | frameshift_variant | 2 | B54,B72 |
Chr7:54416098 |
AG |
A |
1bp_deletion | CDS_variant | 2 | B54,B72 |
Chr7:54418247 |
C |
T |
EMS_canonical_SNV | CDS_variant | 2 | A3,C31 |
Chr7:54418266 |
C |
T |
EMS_canonical_SNV | CDS_variant | 2 | B39,C49 |
Chr7:54418268 |
G |
A |
EMS_canonical_SNV | CDS_variant | 4 | A9,B34,B69,B78 |
Chr7:54418301 |
C |
T |
EMS_canonical_SNV | stop_gained | 29 | A13,A15,A22,A23,A5,B101,B104,B12,B135,B137,B138,B139,B15,B2,B65,C12,C20,C24,C27,C29,C31,C32,C37,C56,C62,C64,C66,C68,D11 |
Chr7:54418398 |
TGA |
T |
2bp_deletion | frameshift_variant | 6 | B18,B77,B92,C21,C54,C83 |
Chr7:54418401 |
A |
ACG |
2bp_insertion | frameshift_variant | 6 | B18,B77,B92,C21,C54,C83 |
Chr7:54418426 |
G |
A |
EMS_canonical_SNV | CDS_variant | 1 | D10 |
Chr7:54418596 |
G |
A |
EMS_canonical_SNV | CDS_variant | 1 | B118 |
Chr7:54418599 |
C |
T |
EMS_canonical_SNV | CDS_variant | 1 | B100 |
结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。
XP_057775443.1PF00390
malic 54-235 aa
PF03949
Malic_M 245-498 aa
| Protein ID | 来源 | 结构域编号 | 结构域名称 | 类型 | 起始 aa | 终止 aa | E-value | Score | 说明 |
|---|---|---|---|---|---|---|---|---|---|
XP_057775443.1 |
Pfam | PF00390 |
malic | Domain | 54 | 235 | 1.6e-79 | 266.7 | Malic enzyme, N-terminal domain |
XP_057775443.1 |
Pfam | PF03949 |
Malic_M | Domain | 245 | 498 | 1.7e-96 | 323.2 | Malic enzyme, NAD binding domain |
每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。
1 records
下载 cDNA FASTA1 records
下载 CDS FASTA1 records
下载 Protein FASTAupstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。