Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr01G003690`

染色体: Chr01 坐标: 55270590-55273486 链方向: + 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr01G003690`
名称	SmilChr01G003690
描述	uncharacterized SmilChr01G003690
染色体 / SeqID	Chr01 / NC_080387.1
坐标与链方向	55270590 - 55273486 / `+`

ID 对照

NCBI Gene ID	`LOC130995225`
GeneID	`130995225`
locus_tag	`-`
NCBI Name	`LOC130995225`
原始查询	`LOC130995225`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	NAD(P)-binding Rossmann-fold superfamily protein [Source:NCBI gene (formerly Entrezgene);Acc:817929]; symbol=AT2G33630; hit=AT2G33630; species=arabidopsis; confidence=high
产物	NAD(P)-binding Rossmann-fold superfamily protein [Source:NCBI gene (formerly Entrezgene);Acc:817929]; symbol=AT2G33630; hit=AT2G33630; species=arabidopsis; confidence=high
Arabidopsis	`AT2G33630` (AT2G33630) NAD(P)-binding Rossmann-fold superfamily protein [Source:NCBI gene (formerly Entrezgene);Acc:817929] identity=70.8; qcov=0.907; evalue=0; confidence=high
Rice	`Os02g0715200` - identity=69.2; qcov=0.909; evalue=0; confidence=high
GO	GO:0003854 (3-beta-hydroxy-Delta5-steroid dehydrogenase (NAD+) activity); GO:0006694 (steroid biosynthetic process)
KEGG	-
Pfam	PF01073 (3Beta_HSD); PF01370 (Epimerase); PF02719 (Polysacc_synt_2); PF04321 (RmlD_sub_bind); PF05368 (NmrA); PF07993 (NAD_binding_4); PF13460 (NAD_binding_10); PF14934 (TMEM254); PF16363 (GDP_Man_Dehyd)
InterPro	-
eggNOG	-
NR	GeneID:130995225

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Endoplasmic reticulum	内质网	0.60000	`XP_057776406.1`
2	Mitochondrion	线粒体	0.40000	`XP_057776406.1`
3	Lipid droplet	脂滴	0.20000	`XP_057776406.1`
4	Nucleus	细胞核	0.00000	`XP_057776406.1`
5	Chromosome	染色体	0.00000	`XP_057776406.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr1:55270759`	`A`	`AC`	1bp_insertion	frameshift_variant	1	B7
`Chr1:55270759`	`A`	`AC`	1bp_insertion	CDS_variant	1	B7
`Chr1:55270769`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	C31,C5
`Chr1:55270797`	`CT`	`C`	1bp_deletion	frameshift_variant	1	B41
`Chr1:55270797`	`CT`	`C`	1bp_deletion	CDS_variant	1	B41
`Chr1:55270827`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B9
`Chr1:55270846`	`AT`	`A`	1bp_deletion	frameshift_variant	1	B130
`Chr1:55270846`	`AT`	`A`	1bp_deletion	CDS_variant	1	B130
`Chr1:55271019`	`C`	`T`	EMS_canonical_SNV	CDS_variant	5	A11,A15,B32,B66,C35
`Chr1:55271596`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B18
`Chr1:55271619`	`G`	`A`	EMS_canonical_SNV	CDS_variant	4	A11,A15,B32,B66
`Chr1:55271711`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B109,C20

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057776406.1

525 aa

262

525

NmrA

Polysacc_synt_2

RmlD_sub_bind

Epimerase

3Beta_HSD

NAD_binding_4

GDP_Man_Dehyd

NAD_binding_10

TMEM254

PF05368 NmrA 57-175 aa PF02719 Polysacc_synt_2 60-175 aa PF04321 RmlD_sub_bind 60-220 aa PF01370 Epimerase 60-286 aa PF01073 3Beta_HSD 60-339 aa PF07993 NAD_binding_4 61-283 aa PF16363 GDP_Man_Dehyd 61-285 aa PF13460 NAD_binding_10 63-178 aa PF14934 TMEM254 428-517 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057776406.1`	Pfam	`PF05368`	NmrA	Family	57	175	9.5e-07	29.4	NmrA-like family
`XP_057776406.1`	Pfam	`PF02719`	Polysacc_synt_2	Family	60	175	5.2e-09	36.4	Polysaccharide biosynthesis protein
`XP_057776406.1`	Pfam	`PF04321`	RmlD_sub_bind	Domain	60	220	1.3e-09	38.3	RmlD substrate binding domain
`XP_057776406.1`	Pfam	`PF01370`	Epimerase	Family	60	286	2.4e-34	119.5	NAD dependent epimerase/dehydratase family
`XP_057776406.1`	Pfam	`PF01073`	3Beta_HSD	Family	60	339	1.6e-84	283.9	3-beta hydroxysteroid dehydrogenase/isomerase family
`XP_057776406.1`	Pfam	`PF07993`	NAD_binding_4	Family	61	283	3.5e-10	40.2	Male sterility protein
`XP_057776406.1`	Pfam	`PF16363`	GDP_Man_Dehyd	Domain	61	285	7.5e-20	72.4	GDP-mannose 4,6 dehydratase
`XP_057776406.1`	Pfam	`PF13460`	NAD_binding_10	Domain	63	178	8.5e-14	52.5	NAD(P)H-binding
`XP_057776406.1`	Pfam	`PF14934`	TMEM254	Domain	428	517	1.6e-23	84.0	Transmembrane protein 254

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

1 records

下载 cDNA FASTA

XM_057920423.1
AGGGCCATTTGAAGCAGAACAGAGGAAGTATAATTTTGGCCATGAAAATGGATATCAACCCCTAAATATTTGCCCAAAAAAAATACAATTATGTTAATTTTCCATACAATCCGCACAAGAAATTCTGTATTTACCAAATTTTGTACTCCAGTATTTGATGCCCCATGTTACCCAAATGCCACGTGTCCTAAAAAGAGAAAAAGGCACCTTCACAAACAGACTGGAAGCACAGAGAGTCGACTCAGGTCATTGTGGCATTT...

CDS

1 records

下载 CDS FASTA

LOC130995225
ATGCCCCATGTTACCCAAATGCCACGTGTCCTAAAAAGAGAAAAAGGCACCTTCACAAACAGACTGGAAGCACAGAGAGTCGACTCAGGTCATTGTGGCATTTTCGCAAACAGATTGCGGGATTTCAATTTCAAGATGCATTTGAGCGAGAACGAGGGGATAGAAGGCAAAACCTTCGTGGTCACCGGCGGCCTGGGCTTTGTGGGCTCCTCCCTCTGCTTCGAGCTGGTCAGACGAGGTGCCTATCGAGTCCGGGCCTT...

Protein

1 records

下载 Protein FASTA

XP_057776406.1
MPHVTQMPRVLKREKGTFTNRLEAQRVDSGHCGIFANRLRDFNFKMHLSENEGIEGKTFVVTGGLGFVGSSLCFELVRRGAYRVRAFDIRAESPWSDALHDRGVRCLQGDVCKRKDVEKALQGADCVFHLASYGMSGKEMLQYGRIDEININGTCHVLDACLEHGVSRLVYVSTYNVVFGGQEVINGNEALPYFPIDDHVDPYGRSKSIAEQLVLKHNDRSFRKEKGKLYSCAIRPAAIYGPGEERHLPRIIKLAKLGLL...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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JBrowse

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