Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr07G002719`

染色体: Chr07 坐标: 26652325-26658739 链方向: + 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr07G002719`
名称	SmilChr07G002719
描述	alpha-amylase 3, chloroplastic
染色体 / SeqID	Chr07 / NC_080393.1
坐标与链方向	26652325 - 26658739 / `+`

ID 对照

NCBI Gene ID	`LOC130995729`
GeneID	`130995729`
locus_tag	`-`
NCBI Name	`LOC130995729`
原始查询	`LOC130995729`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	alpha-amylase 3, chloroplastic
产物	alpha-amylase 3%2C chloroplastic
Arabidopsis	`AT1G69830` (AMY3) alpha-amylase-like 3 [Source:NCBI gene (formerly Entrezgene);Acc:843319] identity=67.5; qcov=1; evalue=0; confidence=high
Rice	`Os01g0715400` (OsSTA30) - identity=61.4; qcov=1.01; evalue=0; confidence=high
GO	GO:0004556 (alpha-amylase activity); GO:0005509 (calcium ion binding); GO:0005975 (carbohydrate metabolic process)
KEGG	smil00500 (Starch and sucrose metabolism - Salvia miltiorrhiza (redroot sage)); smil01100 (Metabolic pathways - Salvia miltiorrhiza (redroot sage)); smil01110 (Biosynthesis of secondary metabolites - Salvia miltiorrhiza (redroot sage))
Pfam	PF00128 (Alpha-amylase); PF07821 (Alpha-amyl_C2); PF23166 (Ig_N_CWD1)
InterPro	-
eggNOG	-
NR	GeneID:130995729

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Chloroplast	叶绿体	1.00000	`XP_057777112.1`
2	Cytosol	细胞质基质	1.00000	`XP_057777113.1`
3	Chloroplast stroma	叶绿体基质	1.00000	`XP_057777112.1`
4	Cytoplasm	细胞质	0.40000	`XP_057777113.1`
5	Nucleus	细胞核	0.00000	`XP_057777112.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr7:26654523`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	A19
`Chr7:26654735`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C35
`Chr7:26655195`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B137
`Chr7:26655287`	`G`	`A`	EMS_canonical_SNV	CDS_variant	4	B28,B58,C78,D11
`Chr7:26657463`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B28,B58,C78
`Chr7:26657465`	`TCA`	`T`	2bp_deletion	frameshift_variant	1	C13
`Chr7:26657465`	`TCA`	`T`	2bp_deletion	CDS_variant	1	C13
`Chr7:26657881`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B8
`Chr7:26658204`	`GGA`	`G`	2bp_deletion	frameshift_variant	3	B2,C32,C68
`Chr7:26658204`	`GGA`	`G`	2bp_deletion	CDS_variant	3	B2,C32,C68
`Chr7:26658261`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B19
`Chr7:26658276`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C27

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057777112.1

911 aa

456

911

Ig_N_CWD1

Alpha-amylase

Alpha-amyl_C2

PF23166 Ig_N_CWD1 90-214 aa PF23166 Ig_N_CWD1 279-393 aa PF00128 Alpha-amylase 541-615 aa PF07821 Alpha-amyl_C2 851-909 aa

XP_057777113.1

767 aa

384

767

Ig_N_CWD1

Alpha-amylase

Alpha-amyl_C2

PF23166 Ig_N_CWD1 1-70 aa PF23166 Ig_N_CWD1 135-249 aa PF00128 Alpha-amylase 397-471 aa PF07821 Alpha-amyl_C2 707-765 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057777112.1`	Pfam	`PF23166`	Ig_N_CWD1	Domain	90	214	1.5e-64	216.3	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
`XP_057777112.1`	Pfam	`PF23166`	Ig_N_CWD1	Domain	279	393	1.5e-64	216.3	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
`XP_057777112.1`	Pfam	`PF00128`	Alpha-amylase	Domain	541	615	5.6e-17	62.8	Alpha amylase, catalytic domain
`XP_057777112.1`	Pfam	`PF07821`	Alpha-amyl_C2	Domain	851	909	1.1e-22	80.8	Alpha-amylase C-terminal beta-sheet domain
`XP_057777113.1`	Pfam	`PF23166`	Ig_N_CWD1	Domain	1	70	3.2e-50	170.1	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
`XP_057777113.1`	Pfam	`PF23166`	Ig_N_CWD1	Domain	135	249	3.2e-50	170.1	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
`XP_057777113.1`	Pfam	`PF00128`	Alpha-amylase	Domain	397	471	3.6e-17	63.4	Alpha amylase, catalytic domain
`XP_057777113.1`	Pfam	`PF07821`	Alpha-amyl_C2	Domain	707	765	8.7e-23	81.1	Alpha-amylase C-terminal beta-sheet domain

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

2 records

下载 cDNA FASTA

XM_057921129.1
AGAAAAAATCAAAATCATGAATATCATATACGCAAACACAATGCCATACCATAATGTGTATATGTATGCCAATGCCAGTGCCTGAAATATCTGAGAACAAATCTACCTTCATCATTCTCCGAATACAATGTCTGTAGTTACGACGGACTCACTCTTCATTCTCCACCACCACCGTCGTCGCAGTAGCTATTTCAAACCCCTCGAAAAGTTGCAACTTGTTCCTAGAAAGCCCATTTCTTTCAACCAAAATTGCACGCACA...

XM_057921130.1
CGCGCCAATGTCCGCTCCCGCCGTTGCTGCGCTCCCAAAGCTCTCAGTTCCTCCGGCACCGCCGTCGTTGAAACGCCCGAAGCTACAGATGTTACCTTTTCTGAAACTTTCGACTTGAAGCGGCGTGAAAAGTTGAATGTATTTATATGCCTCAAAGTATGGAAACAGAAGTTAATATACTTTTTAAAAGCATATTTTTCTTCTTGGTTGGTTTATTGGAGTGACAGCTGGAAGGAAAGATAACAATCAGATTAGAAAGT...

CDS

2 records

下载 CDS FASTA

LOC130995729
ATGTCTGTAGTTACGACGGACTCACTCTTCATTCTCCACCACCACCGTCGTCGCAGTAGCTATTTCAAACCCCTCGAAAAGTTGCAACTTGTTCCTAGAAAGCCCATTTCTTTCAACCAAAATTGCACGCACACGCCCAACCGCCGCGCCAATGTCCGCTCCCGCCGTTGCTGCGCTCCCAAAGCTCTCAGTTCCTCCGGCACCGCCGTCGTTGAAACGCCCGAAGCTACAGATGTTACCTTTTCTGAAACTTTCGACTT...

LOC130995729
ATGAGACCTGCGGGCTCTATTCTCGTTAAGGACTACGCAATTGAGACTCCTTTTGAAAGATCACCTGCTTCATCTGTAGGAGATTCTTTTGTTGAAGTGAAGATTGATTTCGACACGAACAGTTCAATTGCAGCCATAAATTTTGTGCTGAAGGATGAGGAAACTGGGAGTTGGTATCAGCATAGAGGAAGAGATTTTAAAGTTCCTCTTGTCGATTCCCTTCAAGATGATGGCAATGTTGTTGGAGCAAAGAGCTTGGG...

Protein

2 records

下载 Protein FASTA

XP_057777112.1
MSVVTTDSLFILHHHRRRSSYFKPLEKLQLVPRKPISFNQNCTHTPNRRANVRSRRCCAPKALSSSGTAVVETPEATDVTFSETFDLKRREKLEGKITIRLESGKDEEHWSLTVGCSLPGKWVLHWGVNYVGDTGSEWDQPQADMRPAGSILVKDYAIETPFERSPASSVGDSFVEVKIDFDTNSSIAAINFVLKDEETGSWYQHRGRDFKVPLVDSLQDDGNVVGAKSLGVLPGDLGQISNLPLETESADGKGNDIKES...

XP_057777113.1
MRPAGSILVKDYAIETPFERSPASSVGDSFVEVKIDFDTNSSIAAINFVLKDEETGSWYQHRGRDFKVPLVDSLQDDGNVVGAKSLGVLPGDLGQISNLPLETESADGKGNDIKESNLEKRPLQGFYEEHSVFKEICTDNLMSVSVSHCTEKDKHLLYIETDLPGDVVIHWGVCRDEGKNWEVPAEPYPPETIIFKNKALQTLLQQKTDGCGSWGSFTLGDEFSAFVFVLKLDNNTWLNCKGDDFYIPFTSNATKGELSG...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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