当前解析结果
SmilChr08G003083| 统一新 Gene ID | SmilChr08G003083 |
|---|---|
| 名称 | SmilChr08G003083 |
| 描述 | probable DNA helicase MCM8 |
| 染色体 / SeqID | Chr08 / NC_080394.1 |
| 坐标与链方向 | 31046825 - 31054118 / - |
| NCBI Gene ID | LOC130997630 |
|---|---|
| GeneID | 130997630 |
| locus_tag | - |
| NCBI Name | LOC130997630 |
| 原始查询 | LOC130997630 |
| 来源 | GFF basic annotation; NCBI GO GAF; Pfam-A/HMMER; Arabidopsis/Rice homolog |
|---|---|
| 描述 | probable DNA helicase MCM8 |
| 产物 | probable DNA helicase MCM8 |
| Arabidopsis | AT3G09660 (MCM8)minichromosome maintenance 8 [Source:NCBI gene (formerly Entrezgene);Acc:820123] identity=71.7; qcov=0.981; evalue=0; confidence=high |
| Rice | Os05g0464100 (OsMCM8)- identity=64.4; qcov=0.569; evalue=0; confidence=medium |
| GO | GO:0003697 (single-stranded DNA binding); GO:0005524 (ATP binding); GO:0005634 (nucleus); GO:0016887 (ATP hydrolysis activity); GO:0017116 (single-stranded DNA helicase activity); GO:0032508 (GO:0032508); GO:0042555 (MCM complex) |
| KEGG | - |
| Pfam | PF00493 (MCM); PF01078 (Mg_chelatase); PF07728 (AAA_5); PF17207 (MCM_OB); PF17855 (MCM_lid); PF25051 (WHD_MCM8) |
| InterPro | - |
| eggNOG | - |
| NR | GeneID:130997630 |
| Rank | 定位位置 | 中文 | Score | Protein ID |
|---|---|---|---|---|
| 1 | Nucleus | 细胞核 | 1.00000 | XP_057778999.1 |
| 2 | Nucleoplasm | 核质 | 0.60000 | XP_057778999.1 |
| 3 | Chromosome | 染色体 | 0.00000 | XP_057778999.1 |
| 4 | Centromere | 着丝粒 | 0.00000 | XP_057778999.1 |
| 5 | Chromatin | 染色质 | 0.00000 | XP_057778999.1 |
下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。
| 位置 | REF | ALT | 类型 | 影响 | 携带材料数 | 材料 |
|---|---|---|---|---|---|---|
Chr8:31047519 |
G |
A |
EMS_canonical_SNV | CDS_variant | 2 | A22,B101 |
Chr8:31047523 |
C |
T |
EMS_canonical_SNV | CDS_variant | 1 | B68 |
Chr8:31048364 |
G |
A |
EMS_canonical_SNV | CDS_variant | 1 | C8 |
Chr8:31048461 |
G |
A |
EMS_canonical_SNV | CDS_variant | 5 | B108,B28,C1,C23,C43 |
Chr8:31048500 |
G |
A |
EMS_canonical_SNV | CDS_variant | 4 | B108,B28,B35,C23 |
Chr8:31048510 |
G |
A |
EMS_canonical_SNV | CDS_variant | 5 | B108,B28,B35,B72,C23 |
Chr8:31048527 |
C |
T |
EMS_canonical_SNV | CDS_variant | 4 | B108,B117,B28,C23 |
Chr8:31048531 |
G |
A |
EMS_canonical_SNV | CDS_variant | 4 | B108,B117,B28,C23 |
Chr8:31048571 |
CT |
C |
1bp_deletion | frameshift_variant | 2 | B117,C23 |
Chr8:31048571 |
CT |
C |
1bp_deletion | CDS_variant | 2 | B117,C23 |
Chr8:31048582 |
C |
T |
EMS_canonical_SNV | CDS_variant | 3 | B103,B35,B62 |
Chr8:31048586 |
TC |
T |
1bp_deletion | frameshift_variant | 3 | B108,B117,C23 |
结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。
XP_057778999.1PF17207
MCM_OB 138-267 aa
PF00493
MCM 341-555 aa
PF07728
AAA_5 391-529 aa
PF01078
Mg_chelatase 445-533 aa
PF17855
MCM_lid 607-690 aa
PF25051
WHD_MCM8 702-777 aa
| Protein ID | 来源 | 结构域编号 | 结构域名称 | 类型 | 起始 aa | 终止 aa | E-value | Score | 说明 |
|---|---|---|---|---|---|---|---|---|---|
XP_057778999.1 |
Pfam | PF17207 |
MCM_OB | Domain | 138 | 267 | 6.5e-29 | 101.0 | MCM OB domain |
XP_057778999.1 |
Pfam | PF00493 |
MCM | Domain | 341 | 555 | 3.7e-90 | 301.6 | MCM P-loop domain |
XP_057778999.1 |
Pfam | PF07728 |
AAA_5 | Domain | 391 | 529 | 8.3e-07 | 29.8 | AAA domain (dynein-related subfamily) |
XP_057778999.1 |
Pfam | PF01078 |
Mg_chelatase | Domain | 445 | 533 | 5e-08 | 33.3 | Magnesium chelatase, subunit ChlI |
XP_057778999.1 |
Pfam | PF17855 |
MCM_lid | Domain | 607 | 690 | 5.2e-26 | 91.7 | MCM AAA-lid domain |
XP_057778999.1 |
Pfam | PF25051 |
WHD_MCM8 | Domain | 702 | 777 | 1.7e-31 | 108.8 | MCM8 winged helix domain |
每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。
1 records
下载 cDNA FASTA1 records
下载 CDS FASTA1 records
下载 Protein FASTAupstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。