Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr08G004762`

染色体: Chr08 坐标: 56978478-56980816 链方向: - 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr08G004762`
名称	SmilChr08G004762
描述	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase
染色体 / SeqID	Chr08 / NC_080394.1
坐标与链方向	56978478 - 56980816 / `-`

ID 对照

NCBI Gene ID	`LOC130999434`
GeneID	`130999434`
locus_tag	`-`
NCBI Name	`LOC130999434`
原始查询	`LOC130999434`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase
产物	probable 3-beta-hydroxysteroid-Delta(8)%2CDelta(7)-isomerase
Arabidopsis	`AT1G20050` (HYD1) C-8,7 sterol isomerase [Source:NCBI gene (formerly Entrezgene);Acc:838593] identity=67.3; qcov=0.995; evalue=1.23e-94; confidence=high
Rice	`Os01g0103600` - identity=60.5; qcov=0.995; evalue=1.27e-92; confidence=high
GO	GO:0000247 (C-8 sterol isomerase activity); GO:0004769 (steroid Delta-isomerase activity); GO:0005783 (endoplasmic reticulum); GO:0016020 (membrane); GO:0016126 (sterol biosynthetic process); GO:0047750 (cholestenol delta-isomerase activity)
KEGG	smil00100 (Steroid biosynthesis - Salvia miltiorrhiza (redroot sage)); smil01100 (Metabolic pathways - Salvia miltiorrhiza (redroot sage)); smil01110 (Biosynthesis of secondary metabolites - Salvia miltiorrhiza (redroot sage))
Pfam	PF05241 (EBP)
InterPro	-
eggNOG	-
NR	GeneID:130999434

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Endoplasmic reticulum	内质网	1.00000	`XP_057780941.1`
2	Endoplasmic reticulum membrane	内质网膜	1.00000	`XP_057780941.1`
3	Nucleus membrane	核膜	0.20000	`XP_057780941.1`
4	Nucleus	细胞核	0.00000	`XP_057780941.1`
5	Chromosome	染色体	0.00000	`XP_057780941.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr8:56978807`	`G`	`A`	EMS_canonical_SNV	CDS_variant	3	A2,B117,C80
`Chr8:56978816`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	D11
`Chr8:56978831`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B73,C23
`Chr8:56979122`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B68,B76
`Chr8:56979235`	`G`	`A`	EMS_canonical_SNV	CDS_variant	5	A20,B17,B97,C22,D10
`Chr8:56979821`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B13,B78,C85
`Chr8:56979866`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C87
`Chr8:56979867`	`G`	`A`	EMS_canonical_SNV	CDS_variant	4	B89,C43,C82,D10

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057780941.1

221 aa

110

221

EBP

PF05241 EBP 94-202 aa

XP_057780942.1

221 aa

110

221

EBP

PF05241 EBP 94-202 aa

XP_057780943.1

221 aa

110

221

EBP

PF05241 EBP 94-202 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057780941.1`	Pfam	`PF05241`	EBP	Family	94	202	8.1e-24	84.9	EXPERA (EXPanded EBP superfamily)
`XP_057780942.1`	Pfam	`PF05241`	EBP	Family	94	202	8.1e-24	84.9	EXPERA (EXPanded EBP superfamily)
`XP_057780943.1`	Pfam	`PF05241`	EBP	Family	94	202	8.1e-24	84.9	EXPERA (EXPanded EBP superfamily)

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

3 records

下载 cDNA FASTA

XM_057924960.1
ATGCTATCTAAACTAACAGGAAGAAGAAAGAAAAGAAAAAACGTTAAAAAAATTTAAACAACAAATTGTAGGAGAGAATACATCTAGATCTGCTTTGTGCGTCTTCATTGACTTATCACTCCAAAATTCTAGCTGGAGTTAAAACAGTGTAGAGTGTATATACACACACATATCCACTACACTTTTGATGATCCACTCCTTCTTCGCAATCAATTGAACTGTAGCAAATTGTTCATCAGCATATTTCCGTTTTTCGCGTA...

XM_057924959.1
ATGCTATCTAAACTAACAGGAAGAAGAAAGAAAAGAAAAAACGTTAAAAAAATTTAAACAACAAATTGTAGGAGAGAATACATCTAGATCTGCTTTGTGCGTCTTCATTGACTTATCACTCCAAAATTCTAGCTGGAGTTAAAACAGTGTAGAGTGTATATACACACACATATCCACTACACTTTTGATGATCCACTCCTTCTTCGCAATCAATTGAACTGTAGCAAATTGTTCATCAGCATATTTCCAAACAGCAGGTT...

CDS

3 records

下载 CDS FASTA

LOC130999434
ATGAGTATGGACGCCGATCACCCCTACGTGCCAAGAGATCTAAAGTTGCCTGGCTACGTGCCTTTGTTACTCTCTCAGTCAGCAATCCTTGTTGTGTATGGTGCCGCGTCTGTGTTGGTGATCTCCTTAATGTGGCTCTTATCTGGGCGGATTCATAAGATTTCCAAAGTCGATAGGGTGTTAATGTGCTGGTGGATATTCACTGGTCTCACCCACATTATCCTCGAGGGCTACTTTGCCTTCACCCCCGATTTTTACAA...

Protein

3 records

下载 Protein FASTA

XP_057780941.1
MSMDADHPYVPRDLKLPGYVPLLLSQSAILVVYGAASVLVISLMWLLSGRIHKISKVDRVLMCWWIFTGLTHIILEGYFAFTPDFYKVKTTHYLAEVWKEYSKGDSRYVGRDSAVVAVEGITAAIEGPACLLVVYAIAKRKSYSYVLQLSICLGQLYGCVVYFATAVLEGDNFATSMFYYYAYYVFANMWWVIIPGAIAARCWKKICAACQVQEQRKSKAR

XP_057780942.1
MSMDADHPYVPRDLKLPGYVPLLLSQSAILVVYGAASVLVISLMWLLSGRIHKISKVDRVLMCWWIFTGLTHIILEGYFAFTPDFYKVKTTHYLAEVWKEYSKGDSRYVGRDSAVVAVEGITAAIEGPACLLVVYAIAKRKSYSYVLQLSICLGQLYGCVVYFATAVLEGDNFATSMFYYYAYYVFANMWWVIIPGAIAARCWKKICAACQVQEQRKSKAR

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

下载 upstream 下载 downstream 下载 upstream + gene + downstream

JBrowse

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