Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr08G000614`

染色体: Chr08 坐标: 4563620-4583476 链方向: - 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr08G000614`
名称	SmilChr08G000614
描述	DNA polymerase epsilon catalytic subunit A
染色体 / SeqID	Chr08 / NC_080394.1
坐标与链方向	4563620 - 4583476 / `-`

ID 对照

NCBI Gene ID	`LOC131000190`
GeneID	`131000190`
locus_tag	`-`
NCBI Name	`LOC131000190`
原始查询	`LOC131000190`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	DNA polymerase epsilon catalytic subunit A
产物	DNA polymerase epsilon catalytic subunit A
Arabidopsis	`AT1G08260` (TIL1) DNA polymerase epsilon catalytic subunit [Source:NCBI gene (formerly Entrezgene);Acc:837346] identity=69.5; qcov=1; evalue=0; confidence=high
Rice	`Os02g0511900` - identity=81.5; qcov=0.287; evalue=0; confidence=weak
GO	GO:0000166 (nucleotide binding); GO:0000278 (mitotic cell cycle); GO:0003677 (DNA binding); GO:0003887 (DNA-directed DNA polymerase activity); GO:0005634 (nucleus); GO:0006260 (DNA replication); GO:0006272 (leading strand elongation); GO:0006287 (base-excision repair, gap-filling); GO:0006297 (nucleotide-excision repair, DNA gap filling); GO:0008270 (zinc ion binding); GO:0008310 (single-stranded DNA 3'-5' DNA exonuclease activity); GO:0008622 (epsilon DNA polymerase complex); GO:0045004 (DNA replication proofreading)
KEGG	smil03030 (DNA replication - Salvia miltiorrhiza (redroot sage)); smil03410 (Base excision repair - Salvia miltiorrhiza (redroot sage)); smil03420 (Nucleotide excision repair - Salvia miltiorrhiza (redroot sage))
Pfam	PF00136 (DNA_pol_B); PF03104 (DNA_pol_B_exo1); PF08490 (DUF1744); PF22634 (POL2_thumb); PF22912 (zf-DPOE); PF23250 (zf_DPOE_2)
InterPro	-
eggNOG	-
NR	GeneID:131000190

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Cytoplasm	细胞质	1.00000	`XP_057781956.1`
2	Nucleus	细胞核	0.80000	`XP_057781956.1`
3	Cytosol	细胞质基质	0.40000	`XP_057781956.1`
4	Chromosome	染色体	0.00000	`XP_057781956.1`
5	Centromere	着丝粒	0.00000	`XP_057781956.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr8:4564043`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B85
`Chr8:4564076`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	D11
`Chr8:4564125`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B10
`Chr8:4564172`	`G`	`A`	EMS_canonical_SNV	CDS_variant	3	B101,B56,D7
`Chr8:4564193`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B14
`Chr8:4565402`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	D11
`Chr8:4566224`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B131
`Chr8:4567265`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B10,C80,D11
`Chr8:4567872`	`C`	`T`	EMS_canonical_SNV	CDS_variant	5	A6,B115,B135,B91,C71
`Chr8:4567908`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	D11
`Chr8:4568039`	`G`	`GC`	1bp_insertion	frameshift_variant	3	B10,C80,D11
`Chr8:4568039`	`G`	`GC`	1bp_insertion	CDS_variant	3	B10,C80,D11

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057781956.1

2217 aa

1108

2217

DNA_pol_B_exo1

DNA_pol_B

POL2_thumb

DUF1744

zf_DPOE_2

zf-DPOE

PF03104 DNA_pol_B_exo1 196-402 aa PF00136 DNA_pol_B 757-1073 aa PF22634 POL2_thumb 948-1123 aa PF08490 DUF1744 1525-1893 aa PF23250 zf_DPOE_2 2078-2144 aa PF22912 zf-DPOE 2150-2206 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057781956.1`	Pfam	`PF03104`	DNA_pol_B_exo1	Family	196	402	1.7e-55	188.8	DNA polymerase family B, exonuclease domain
`XP_057781956.1`	Pfam	`PF00136`	DNA_pol_B	Family	757	1073	6.5e-19	68.8	DNA polymerase family B
`XP_057781956.1`	Pfam	`PF22634`	POL2_thumb	Domain	948	1123	4.8e-77	258.6	DNA polymerase epsilon catalytic subunit A, thumb domain
`XP_057781956.1`	Pfam	`PF08490`	DUF1744	Domain	1525	1893	1.8e-120	403.0	Domain of unknown function (DUF1744)
`XP_057781956.1`	Pfam	`PF23250`	zf_DPOE_2	Domain	2078	2144	3.8e-19	68.8	Zinc finger domain of DNA polymerase-epsilon
`XP_057781956.1`	Pfam	`PF22912`	zf-DPOE	Domain	2150	2206	5.8e-15	55.9	Zinc finger domain of DNA polymerase-epsilon

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

1 records

下载 cDNA FASTA

XM_057925973.1
GATCCTCTGCCCTCACAGTCTCCCCGCTTCTCTCTCCCTCTCTTCACTCTCTTGTCCTGCTTTCAACTTTCTCTTTCTACTAATCCATCTCCATATTCTACTTCGTAGTTCTAACATTTTATTTTTCACACTTTTATTTCACTTGATTAATTTTCATTTTGAGATATACGAGTTTCCTGTAAAGATGATGAATGGCAGCGACAACAGGAGGAAAGATCGGTGGGAATCTCGTATTTCAAAGAAGCAGCAGCTGATTCTAA...

CDS

1 records

下载 CDS FASTA

LOC131000190
ATGATGAATGGCAGCGACAACAGGAGGAAAGATCGGTGGGAATCTCGTATTTCAAAGAAGCAGCAGCTGATTCTAAATGCAGAGGAGCAGCTTGAATCCAAGCTCGGATTCGATCTCTTTACCGAAGGCGATAAGCGCCTGGGCTGGTTACTTACTTTGGCCTCTTCGTCTTGGGAGGACCGGGAAACTAGTAAGGTGTACAGTTGCGTCGATTTGTATTTTGTTTGCCAGGATGGTTCAATGTTTAAGGCGAAGTATAA...

Protein

1 records

下载 Protein FASTA

XP_057781956.1
MMNGSDNRRKDRWESRISKKQQLILNAEEQLESKLGFDLFTEGDKRLGWLLTLASSSWEDRETSKVYSCVDLYFVCQDGSMFKAKYKFRPYFYAATKDKMETDVEAYLRRRYEGQIADIEIVEKEDLDLKNHLSGLHKCYLKISFDTVQQLMQVKKDLVHVVERNKAKLDAEDAYESILAGKSKQRLQDFIDCINDLREFDVPYHVRFAIDKDIRCGQWYDVSVSSAGILVERRTDLLQRAEVHVCAFDIETTKLPLKFP...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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JBrowse

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