Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr08G002051`

染色体: Chr08 坐标: 18339316-18343976 链方向: - 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr08G002051`
名称	SmilChr08G002051
描述	calcium-dependent protein kinase 18-like
染色体 / SeqID	Chr08 / NC_080394.1
坐标与链方向	18339316 - 18343976 / `-`

ID 对照

NCBI Gene ID	`LOC131001562`
GeneID	`131001562`
locus_tag	`-`
NCBI Name	`LOC131001562`
原始查询	`LOC131001562`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	calcium-dependent protein kinase 18-like
产物	calcium-dependent protein kinase 18-like
Arabidopsis	`AT5G66210` (CPK28) calcium-dependent protein kinase 28 [Source:NCBI gene (formerly Entrezgene);Acc:836753] identity=74; qcov=0.99; evalue=0; confidence=high
Rice	`Os07g0409900` (CDPK18) - identity=84; qcov=0.826; evalue=0; confidence=high
GO	GO:0004683 (calcium/calmodulin-dependent protein kinase activity); GO:0005509 (calcium ion binding); GO:0005516 (calmodulin binding); GO:0005524 (ATP binding); GO:0005634 (nucleus); GO:0005737 (cytoplasm); GO:0009931 (calcium-dependent protein serine/threonine kinase activity); GO:0018105 (peptidyl-serine phosphorylation); GO:0035556 (intracellular signal transduction); GO:0046777 (protein autophosphorylation)
KEGG	smil04075 (Plant hormone signal transduction - Salvia miltiorrhiza (redroot sage)); smil04626 (Plant-pathogen interaction - Salvia miltiorrhiza (redroot sage))
Pfam	PF00069 (Pkinase); PF07714 (PK_Tyr_Ser-Thr); PF10591 (SPARC_Ca_bdg); PF13202 (EF-hand_5); PF13405 (EF-hand_6); PF13499 (EF-hand_7); PF13833 (EF-hand_8)
InterPro	-
eggNOG	-
NR	GeneID:131001562

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Cell membrane	细胞膜	1.00000	`XP_057784010.1`
2	Cytosol	细胞质基质	0.40000	`XP_057784010.1`
3	Nucleus	细胞核	0.00000	`XP_057784010.1`
4	Chromosome	染色体	0.00000	`XP_057784010.1`
5	Centromere	着丝粒	0.00000	`XP_057784010.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr8:18339767`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C39
`Chr8:18340103`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	A20,B71,C80
`Chr8:18340347`	`TG`	`T`	1bp_deletion	frameshift_variant	1	B27
`Chr8:18340347`	`TG`	`T`	1bp_deletion	CDS_variant	1	B27
`Chr8:18340469`	`G`	`A`	EMS_canonical_SNV	CDS_variant	3	B27,C20,C67
`Chr8:18341880`	`G`	`A`	EMS_canonical_SNV	CDS_variant	3	C39,C40,C59
`Chr8:18343318`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C10

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057784010.1

575 aa

288

575

Pkinase

PK_Tyr_Ser-Thr

EF-hand_7

EF-hand_6

EF-hand_5

EF-hand_8

SPARC_Ca_bdg

EF-hand_7

EF-hand_5

EF-hand_8

PF00069 Pkinase 111-371 aa PF07714 PK_Tyr_Ser-Thr 113-364 aa PF13499 EF-hand_7 417-479 aa PF13405 EF-hand_6 418-444 aa PF13202 EF-hand_5 419-441 aa PF13833 EF-hand_8 431-479 aa PF10591 SPARC_Ca_bdg 484-548 aa PF13499 EF-hand_7 499-552 aa PF13202 EF-hand_5 500-519 aa PF13202 EF-hand_5 531-552 aa PF13833 EF-hand_8 531-552 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057784010.1`	Pfam	`PF00069`	Pkinase	Domain	111	371	5e-71	239.9	Protein kinase domain
`XP_057784010.1`	Pfam	`PF07714`	PK_Tyr_Ser-Thr	Domain	113	364	1.2e-29	104.2	Protein tyrosine and serine/threonine kinase
`XP_057784010.1`	Pfam	`PF13499`	EF-hand_7	Domain	417	479	1.1e-24	87.2	EF-hand domain pair
`XP_057784010.1`	Pfam	`PF13405`	EF-hand_6	Domain	418	444	1.1e-21	75.1	EF-hand domain
`XP_057784010.1`	Pfam	`PF13202`	EF-hand_5	Domain	419	441	4.6e-25	86.6	EF hand
`XP_057784010.1`	Pfam	`PF13833`	EF-hand_8	Domain	431	479	3e-14	53.2	EF-hand domain pair
`XP_057784010.1`	Pfam	`PF10591`	SPARC_Ca_bdg	Domain	484	548	5.9e-05	24.1	Secreted protein acidic and rich in cysteine Ca binding region
`XP_057784010.1`	Pfam	`PF13499`	EF-hand_7	Domain	499	552	1.1e-24	87.2	EF-hand domain pair
`XP_057784010.1`	Pfam	`PF13202`	EF-hand_5	Domain	500	519	4.6e-25	86.6	EF hand
`XP_057784010.1`	Pfam	`PF13202`	EF-hand_5	Domain	531	552	4.6e-25	86.6	EF hand
`XP_057784010.1`	Pfam	`PF13833`	EF-hand_8	Domain	531	552	3e-14	53.2	EF-hand domain pair

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

1 records

下载 cDNA FASTA

XM_057928027.1
TAGACAATAATGAAGGTAAGAAAAGAGGGGGAGCTTTTGGCAACCCCCTCTTCCTCTCTCCTCTCTCTCTCTCTCCTGACCAAAAGTCTCTTTGACCATTGCCCACCACCTCCATCTCCTTCTTCCCTTCTTCCTCCTATCTGCTTTAATCTTTCCTCTTGTAATAAATAATACTCCATGTCTCATCATTTCAGATTATATTCCCTCCAATATATATTCCTCCCCTTTTGTTCAATTTATCCACCATGTCCATGTGTGTG...

CDS

1 records

下载 CDS FASTA

LOC131001562
ATGGGTAGCTGTTTTTCAACCACAAAAGTTAGTGGTTCCAATAGCAACACCCCTTCAACCACCACCACCACCGCCACTGCGGTCAACAACCGCCGCTCCTCCTCCGCCGCCGCAGCCAAGCCAGCCTCCGCAACCGCAAACGCTCCGGCAGCTGGGCCGAGGAAGCAGGAGAGCTCTCACAATAATCATCATCAGAAAGCAAGAGAGAATCACAAGAGTCAGCAGCAAAAGCCATCTCAGAATCTCAAGAATTCTTCCAA...

Protein

1 records

下载 Protein FASTA

XP_057784010.1
MGSCFSTTKVSGSNSNTPSTTTTTATAVNNRRSSSAAAAKPASATANAPAAGPRKQESSHNNHHQKARENHKSQQQKPSQNLKNSSKRPSGAIPCGKRTDFGYDKDFEKRYTIGKLLGHGQFGYTYVAIDKSNGDRVAVKKIDKSKMVLPIAVEDVKREVKILRALTGHENVVHFYNAFEDDSYVYIVMELCEGGELLDRILSKKDSRYTEKDAAIVVRQMLKVAAECHLHGFVHRDMKPENFLFKSPKEDSHLKATDFG...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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JBrowse

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