Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr01G006063`

染色体: Chr01 坐标: 76054006-76058197 链方向: + 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr01G006063`
名称	SmilChr01G006063
描述	leucine--tRNA ligase, cytoplasmic-like
染色体 / SeqID	Chr01 / NC_080387.1
坐标与链方向	76054006 - 76058197 / `+`

ID 对照

NCBI Gene ID	`LOC131006313`
GeneID	`131006313`
locus_tag	`-`
NCBI Name	`LOC131006313`
原始查询	`LOC131006313`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	leucine--tRNA ligase, cytoplasmic-like
产物	leucine--tRNA ligase%2C cytoplasmic-like
Arabidopsis	`AT1G09620` (AT1G09620) ATP binding/leucine-tRNA ligases/aminoacyl-tRNA ligase [Source:NCBI gene (formerly Entrezgene);Acc:837489] identity=70.4; qcov=0.938; evalue=0; confidence=high
Rice	`Os09g0503400` - identity=72.7; qcov=0.938; evalue=0; confidence=high
GO	GO:0002161 (aminoacyl-tRNA deacylase activity); GO:0004823 (leucine-tRNA ligase activity); GO:0005524 (ATP binding); GO:0006429 (leucyl-tRNA aminoacylation)
KEGG	smil00970 (Aminoacyl-tRNA biosynthesis - Salvia miltiorrhiza (redroot sage))
Pfam	PF00133 (tRNA-synt_1); PF01406 (tRNA-synt_1e); PF08264 (Anticodon_1); PF09334 (tRNA-synt_1g); PF24810 (RBD_LARS1)
InterPro	-
eggNOG	-
NR	GeneID:131006313

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Cytosol	细胞质基质	0.60000	`XP_057789498.1`
2	Mitochondrion	线粒体	0.60000	`XP_057789498.1`
3	Chloroplast	叶绿体	0.40000	`XP_057789498.1`
4	Nucleus	细胞核	0.00000	`XP_057789498.1`
5	Chromosome	染色体	0.00000	`XP_057789498.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr1:76054314`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B33,B79
`Chr1:76054822`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C82
`Chr1:76054823`	`G`	`A`	EMS_canonical_SNV	CDS_variant	5	A8,B8,B83,B91,C36
`Chr1:76055232`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B122
`Chr1:76055353`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	D11
`Chr1:76055455`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C68
`Chr1:76055505`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B86
`Chr1:76055636`	`C`	`T`	EMS_canonical_SNV	CDS_variant	5	A2,B48,B53,C51,D9
`Chr1:76056020`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B125,B90,D10
`Chr1:76056119`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B17,C5
`Chr1:76056219`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C84
`Chr1:76056358`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B46

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057789498.1

1154 aa

577

1154

tRNA-synt_1

tRNA-synt_1g

tRNA-synt_1

tRNA-synt_1g

tRNA-synt_1e

Anticodon_1

RBD_LARS1

PF00133 tRNA-synt_1 91-174 aa PF09334 tRNA-synt_1g 121-180 aa PF00133 tRNA-synt_1 268-827 aa PF09334 tRNA-synt_1g 736-833 aa PF01406 tRNA-synt_1e 742-817 aa PF08264 Anticodon_1 867-990 aa PF24810 RBD_LARS1 1016-1093 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057789498.1`	Pfam	`PF00133`	tRNA-synt_1	Domain	91	174	4.7e-29	102.1	tRNA synthetases class I (I, L, M and V)
`XP_057789498.1`	Pfam	`PF09334`	tRNA-synt_1g	Family	121	180	4.7e-16	59.3	tRNA synthetases class I (M)
`XP_057789498.1`	Pfam	`PF00133`	tRNA-synt_1	Domain	268	827	4.7e-29	102.1	tRNA synthetases class I (I, L, M and V)
`XP_057789498.1`	Pfam	`PF09334`	tRNA-synt_1g	Family	736	833	4.7e-16	59.3	tRNA synthetases class I (M)
`XP_057789498.1`	Pfam	`PF01406`	tRNA-synt_1e	Domain	742	817	1.4e-08	35.2	tRNA synthetases class I (C) catalytic domain
`XP_057789498.1`	Pfam	`PF08264`	Anticodon_1	Domain	867	990	5.1e-14	53.1	Anticodon-binding domain of tRNA ligase
`XP_057789498.1`	Pfam	`PF24810`	RBD_LARS1	Domain	1016	1093	5.1e-08	33.6	Leucine--tRNA ligase, RagD-binding domain

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

1 records

下载 cDNA FASTA

XM_057933515.1
ATTGTGAAAATATATGCCCAAATAAATAGCCTCTCAAAACCGGGTGGCCCGGAAATTAAACCCGACCGGGTCAGTAACTTTCACTGCGTAGCTAGTAGCTAGCTACTATTTACTGTAGCAATATATAGATACGCGGTAAAAATAATGTATGCATACACTCACCAAAACCCCATTTCCTTACTCTGCGTTTCGGCCTGCTGCTTTTCTTATCTCCGCCCTTCAACTGTGGCGTCTCGTTTCTTATTGAGACGATTTGCTCT...

CDS

1 records

下载 CDS FASTA

LOC131006313
ATGTATGCATACACTCACCAAAACCCCATTTCCTTACTCTGCGTTTCGGCCTGCTGCTTTTCTTATCTCCGCCCTTCAACTGTGGCGTCTCGTTTCTTATTGAGACGATTTGCTCTTCCTCGCTCCATATCCCCCTTTTCTCTTTATTTTCCAATTCCACACTTCATTCCTACCAAATCGAGTTTTTGTGTGCTAGCCGGAAAGGATATGACGGAAGAAACTGGAAAAAGCTTTGCTCGGAGGGACCGTCTATTGGAAAT...

Protein

1 records

下载 Protein FASTA

XP_057789498.1
MYAYTHQNPISLLCVSACCFSYLRPSTVASRFLLRRFALPRSISPFSLYFPIPHFIPTKSSFCVLAGKDMTEETGKSFARRDRLLEIESQVQKWWSEGDVFRADPKDSPPKTGEKFFGNFPFPYMNGHLHLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLTREIEKFGNPPVFPVLKEEEISVPEAKPEGESEGGQNQAGGKFKGKKSKTVAKTGVVKYQWEIMQSYGLTDEEIAKFTNPYHWLTF...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

下载 upstream 下载 downstream 下载 upstream + gene + downstream

JBrowse

跳转到基因组浏览器