Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr02G005266`

染色体: Chr02 坐标: 73745541-73756597 链方向: + 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr02G005266`
名称	SmilChr02G005266
描述	DNA-directed RNA polymerase II subunit RPB2-like
染色体 / SeqID	Chr02 / NC_080388.1
坐标与链方向	73745541 - 73756597 / `+`

ID 对照

NCBI Gene ID	`LOC131009192`
GeneID	`131009192`
locus_tag	`-`
NCBI Name	`LOC131009192`
原始查询	`LOC131009192`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	DNA-directed RNA polymerase II subunit RPB2-like
产物	DNA-directed RNA polymerase II subunit RPB2-like
Arabidopsis	`AT4G21710` (NRPB2) DNA-directed RNA polymerase family protein [Source:NCBI gene (formerly Entrezgene);Acc:828259] identity=88.6; qcov=0.979; evalue=0; confidence=high
Rice	`Os03g0403100` - identity=36; qcov=0.977; evalue=0; confidence=medium
GO	GO:0003677 (DNA binding); GO:0003899 (DNA-directed RNA polymerase activity); GO:0005665 (RNA polymerase II, core complex); GO:0006351 (DNA-templated transcription); GO:0032549 (ribonucleoside binding)
KEGG	smil03020 (RNA polymerase - Salvia miltiorrhiza (redroot sage)); smil03420 (Nucleotide excision repair - Salvia miltiorrhiza (redroot sage))
Pfam	PF00562 (RNA_pol_Rpb2_6); PF04560 (RNA_pol_Rpb2_7); PF04561 (RNA_pol_Rpb2_2); PF04563 (RNA_pol_Rpb2_1); PF04565 (RNA_pol_Rpb2_3); PF04566 (RNA_pol_Rpb2_4); PF04567 (RNA_pol_Rpb2_5)
InterPro	-
eggNOG	-
NR	GeneID:131009192

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Nucleus	细胞核	1.00000	`XP_057792427.1`
2	Nucleolus	核仁	0.40000	`XP_057792427.1`
3	Nucleolar fibrillar center	Nucleolar fibrillar center	0.40000	`XP_057792427.1`
4	Nucleoplasm	核质	0.40000	`XP_057792427.1`
5	Chromosome	染色体	0.20000	`XP_057792427.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr2:73745855`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C38
`Chr2:73745872`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B136
`Chr2:73745884`	`G`	`GA`	1bp_insertion	frameshift_variant	4	C2,C32,C68,C74
`Chr2:73745884`	`G`	`GA`	1bp_insertion	CDS_variant	4	C2,C32,C68,C74
`Chr2:73745907`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B104,C38
`Chr2:73745994`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B104
`Chr2:73747023`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B132,C47,C57
`Chr2:73747083`	`G`	`A`	EMS_canonical_SNV	CDS_variant	5	A21,B24,B46,B75,C15
`Chr2:73747096`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	D10
`Chr2:73747303`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B46,B75
`Chr2:73747732`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B129,D6
`Chr2:73747801`	`G`	`A`	EMS_canonical_SNV	CDS_variant	3	B75,B81,C17

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057792427.1

1196 aa

598

1196

RNA_pol_Rpb2_1

RNA_pol_Rpb2_2

RNA_pol_Rpb2_3

RNA_pol_Rpb2_4

RNA_pol_Rpb2_5

RNA_pol_Rpb2_6

RNA_pol_Rpb2_7

PF04563 RNA_pol_Rpb2_1 42-451 aa PF04561 RNA_pol_Rpb2_2 212-403 aa PF04565 RNA_pol_Rpb2_3 476-540 aa PF04566 RNA_pol_Rpb2_4 576-636 aa PF04567 RNA_pol_Rpb2_5 661-712 aa PF00562 RNA_pol_Rpb2_6 719-1089 aa PF04560 RNA_pol_Rpb2_7 1091-1182 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057792427.1`	Pfam	`PF04563`	RNA_pol_Rpb2_1	Family	42	451	2.9e-77	259.5	RNA polymerase beta subunit
`XP_057792427.1`	Pfam	`PF04561`	RNA_pol_Rpb2_2	Domain	212	403	2e-52	178.3	RNA polymerase Rpb2, domain 2
`XP_057792427.1`	Pfam	`PF04565`	RNA_pol_Rpb2_3	Domain	476	540	2.5e-25	89.1	RNA polymerase Rpb2, domain 3
`XP_057792427.1`	Pfam	`PF04566`	RNA_pol_Rpb2_4	Domain	576	636	2.9e-23	82.4	RNA polymerase Rpb2, domain 4
`XP_057792427.1`	Pfam	`PF04567`	RNA_pol_Rpb2_5	Domain	661	712	3.4e-19	70.2	RNA polymerase Rpb2, domain 5
`XP_057792427.1`	Pfam	`PF00562`	RNA_pol_Rpb2_6	Domain	719	1089	1.7e-141	472.4	RNA polymerase Rpb2, domain 6
`XP_057792427.1`	Pfam	`PF04560`	RNA_pol_Rpb2_7	Domain	1091	1182	1.5e-35	122.2	RNA polymerase Rpb2, domain 7

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

1 records

下载 cDNA FASTA

XM_057936444.1
ATGGAAGCTAAGAACTCAAAATTACGTAGTCTTACGCAGCAGCGCCTTCAACGCTCAACCAACTCCAAACACATGGAAAATCCAAGCCAACTTTCCGGCATCTCTGCCTCAATTCTGTTACGATTTCAGTTTTCAACTCACTCAAATTTGAAATCCGAACCTAAATTTGAAGCTCTCGCGAGAGTAATTTTTCTGTTGAGGGAAAAAACAAATATAGTATCAGAGAACTTAATTTCCGATCGAGAATCATCGTAGGTTTA...

CDS

1 records

下载 CDS FASTA

LOC131009192
ATGTACGGCGGCGGCGGAGGCGGCGAGGAGGAGGCGTACGCGGCGGCGGAGGTGGACGACGGAGCGGACGAGATATCGCAGGAGGACACGTGGACGGTGATCAGCTCGTATTTTGAGGAGAAGGGTTTGGTGAGGCAGCAATTGGACTCGTTCGACGAGTTCATACAGAACACCATGCAGGAGATTGTCGACGAGTCCGCACACATCGAGATCCGACCCGAATCGCAGCACAATCCCGGTCGGAGCTCTGAATTCGCCGA...

Protein

1 records

下载 Protein FASTA

XP_057792427.1
MYGGGGGGEEEAYAAAEVDDGADEISQEDTWTVISSYFEEKGLVRQQLDSFDEFIQNTMQEIVDESAHIEIRPESQHNPGRSSEFAETIYRISFGQIYLSKPMMTESDGETNTLYPKAARLRNLTYSSPLYVDVTKRVIRKGYDCEEVIETQEFTKIFIGKVPIMLRSSYCSLYNLSEKDLMEMGECPYDQGGYFIINGSEKVLIAQEKMSTNHVYVFKKRQPNKYAYVAEVRSIAEAQNKAPSGMFVRMLSRPGAKGGS...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

下载 upstream 下载 downstream 下载 upstream + gene + downstream

JBrowse

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