Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr02G004253`

染色体: Chr02 坐标: 64706455-64712404 链方向: + 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr02G004253`
名称	SmilChr02G004253
描述	probable alpha-amylase 2
染色体 / SeqID	Chr02 / NC_080388.1
坐标与链方向	64706455 - 64712404 / `+`

ID 对照

NCBI Gene ID	`LOC131013001`
GeneID	`131013001`
locus_tag	`-`
NCBI Name	`LOC131013001`
原始查询	`LOC131013001`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	probable alpha-amylase 2
产物	probable alpha-amylase 2
Arabidopsis	`AT1G76130` (AMY2) alpha-amylase-like 2 [Source:NCBI gene (formerly Entrezgene);Acc:843945] identity=80.2; qcov=0.989; evalue=0; confidence=high
Rice	`Os01g0715400` (OsSTA30) - identity=51.5; qcov=0.958; evalue=3.72e-121; confidence=high
GO	GO:0004556 (alpha-amylase activity); GO:0005509 (calcium ion binding); GO:0005975 (carbohydrate metabolic process)
KEGG	smil00500 (Starch and sucrose metabolism - Salvia miltiorrhiza (redroot sage)); smil01100 (Metabolic pathways - Salvia miltiorrhiza (redroot sage)); smil01110 (Biosynthesis of secondary metabolites - Salvia miltiorrhiza (redroot sage))
Pfam	PF00128 (Alpha-amylase); PF07821 (Alpha-amyl_C2)
InterPro	-
eggNOG	-
NR	GeneID:131013001

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Secreted	分泌到胞外	0.60000	`XP_057796984.1`
2	Cytosol	细胞质基质	0.40000	`XP_057796987.1`
3	Vacuole	液泡	0.20000	`XP_057796984.1`
4	Nucleus	细胞核	0.00000	`XP_057796984.1`
5	Chromosome	染色体	0.00000	`XP_057796984.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr2:64707599`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B113
`Chr2:64708942`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	C56,C65
`Chr2:64709769`	`AAT`	`A`	2bp_deletion	frameshift_variant	1	B16
`Chr2:64709769`	`AAT`	`A`	2bp_deletion	CDS_variant	1	B16
`Chr2:64710698`	`G`	`A`	EMS_canonical_SNV	stop_gained	12	A7,B133,B31,B68,B73,B91,C17,C25,C29,C42,C6,C60
`Chr2:64711105`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C54
`Chr2:64712073`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B109
`Chr2:64712074`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C38

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057796984.1

410 aa

205

410

Alpha-amylase

Alpha-amyl_C2

PF00128 Alpha-amylase 42-112 aa PF07821 Alpha-amyl_C2 354-409 aa

XP_057796985.1

410 aa

205

410

Alpha-amylase

Alpha-amyl_C2

PF00128 Alpha-amylase 42-112 aa PF07821 Alpha-amyl_C2 354-409 aa

XP_057796986.1

410 aa

205

410

Alpha-amylase

Alpha-amyl_C2

PF00128 Alpha-amylase 42-112 aa PF07821 Alpha-amyl_C2 354-409 aa

XP_057796987.1

353 aa

176

353

Alpha-amylase

PF00128 Alpha-amylase 42-112 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057796984.1`	Pfam	`PF00128`	Alpha-amylase	Domain	42	112	8.1e-10	39.2	Alpha amylase, catalytic domain
`XP_057796984.1`	Pfam	`PF07821`	Alpha-amyl_C2	Domain	354	409	2.8e-21	76.3	Alpha-amylase C-terminal beta-sheet domain
`XP_057796985.1`	Pfam	`PF00128`	Alpha-amylase	Domain	42	112	8.1e-10	39.2	Alpha amylase, catalytic domain
`XP_057796985.1`	Pfam	`PF07821`	Alpha-amyl_C2	Domain	354	409	2.8e-21	76.3	Alpha-amylase C-terminal beta-sheet domain
`XP_057796986.1`	Pfam	`PF00128`	Alpha-amylase	Domain	42	112	8.1e-10	39.2	Alpha amylase, catalytic domain
`XP_057796986.1`	Pfam	`PF07821`	Alpha-amyl_C2	Domain	354	409	2.8e-21	76.3	Alpha-amylase C-terminal beta-sheet domain
`XP_057796987.1`	Pfam	`PF00128`	Alpha-amylase	Domain	42	112	4.4e-10	40.1	Alpha amylase, catalytic domain

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

4 records

下载 cDNA FASTA

XM_057941002.1
CTCGATGCACCCCGCCTTACCGCGCATTCCATTTACCGTTGAAAACAGACAAATGTGAAGAGCGTCGAATCGAATGTCGTAAAAGTCGAACCCCTTTTCTGAAAGTTCCGATTCTCTGCTCCACTTCGCTCGGCGACGTTCAAATCTCAGTTGATTGAGTTTATTCCTTCTCATAATCCCACCTTTGTGGTCCCCCTTTCTCCTCATTCATGGCTGCTAACTGTACCAGGATTCCAATTCCTCTACTTTCATCTCTTCTC...

XM_057941003.1
CTCGATGCACCCCGCCTTACCGCGCATTCCATTTACCGTTGAAAACAGACAAATGTGAAGAGCGTCGAATCGAATGTCGTAAAAGTCGAACCCCTTTTCTGAAAGTTCCGATTCTCTGCTCCACTTCGCTCGGCGACGTTCAAATCTCAGTTGATTGAGTTTATTCCTTCTCATAATCCCACCTTTGTGGTCCCCCTTTCTCCTCATTCATGGCTGCTAACTGTACCAGGATTCCAATTCCTCTACTTTCATCTCTTCTC...

CDS

4 records

下载 CDS FASTA

LOC131013001
ATGGGAAATGAATTCGGCCGTAGCAATGGGCAAGATGATCCTGCTGCAGCATTACGCAATGGGAAAGAAATTATATTACAGGCCTTCAATTGGGAATCTCATAAACATGATTGGTGGAGGAACTTAGAGCAAAAGGTTCCTGATATAGCGAAATCTGGTTTTACATCAGCTTGGTTGCCCCCACCAACTCATTCATTTTCACCAGAAGGTTATCTTCCCCAGAACCTTTATTCTCTCAACTCTGTATATGGTTCTGAGAA...

Protein

4 records

下载 Protein FASTA

XP_057796984.1
MGNEFGRSNGQDDPAAALRNGKEIILQAFNWESHKHDWWRNLEQKVPDIAKSGFTSAWLPPPTHSFSPEGYLPQNLYSLNSVYGSENLLKALLNKMKTYKVRAMADIVINHRVGTTQGHGGMYNRYDGIPLSWDERAVTGCTGGKGNKSTGDNFHGVPNIDHTQEFVRKDIKDWLRWLRNTVGFQDFRFDFARGYSPKYVKEYIEGAKPIFCVGEHWDSCNYNGTYLEYNQDSHRQRIINWIDSTGQLSSAFDFTTKGIL...

XP_057796985.1
MGNEFGRSNGQDDPAAALRNGKEIILQAFNWESHKHDWWRNLEQKVPDIAKSGFTSAWLPPPTHSFSPEGYLPQNLYSLNSVYGSENLLKALLNKMKTYKVRAMADIVINHRVGTTQGHGGMYNRYDGIPLSWDERAVTGCTGGKGNKSTGDNFHGVPNIDHTQEFVRKDIKDWLRWLRNTVGFQDFRFDFARGYSPKYVKEYIEGAKPIFCVGEHWDSCNYNGTYLEYNQDSHRQRIINWIDSTGQLSSAFDFTTKGIL...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

下载 upstream 下载 downstream 下载 upstream + gene + downstream

JBrowse

跳转到基因组浏览器