Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr03G003514`

染色体: Chr03 坐标: 53741003-53748268 链方向: + 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr03G003514`
名称	SmilChr03G003514
描述	vacuolar protein sorting-associated protein 36
染色体 / SeqID	Chr03 / NC_080389.1
坐标与链方向	53741003 - 53748268 / `+`

ID 对照

NCBI Gene ID	`LOC131014404`
GeneID	`131014404`
locus_tag	`-`
NCBI Name	`LOC131014404`
原始查询	`LOC131014404`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	vacuolar protein sorting-associated protein 36
产物	vacuolar protein sorting-associated protein 36
Arabidopsis	`AT5G04920` (AT5G04920) EAP30/Vps36 family protein [Source:NCBI gene (formerly Entrezgene);Acc:830374] identity=64; qcov=1.01; evalue=0; confidence=high
Rice	`Os01g0813500` - identity=55.5; qcov=1.02; evalue=1.12e-157; confidence=high
GO	GO:0000814 (ESCRT II complex); GO:0031902 (late endosome membrane); GO:0032266 (phosphatidylinositol-3-phosphate binding); GO:0043130 (ubiquitin binding); GO:0043328 (protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway)
KEGG	smil04144 (Endocytosis - Salvia miltiorrhiza (redroot sage))
Pfam	PF04157 (EAP30); PF11605 (Vps36_ESCRT-II)
InterPro	-
eggNOG	-
NR	GeneID:131014404

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Golgi apparatus	高尔基体	1.00000	`XP_057798343.1`
2	Cytoplasm	细胞质	0.80000	`XP_057798343.1`
3	Trans-golgi network	Trans-golgi network	0.80000	`XP_057798343.1`
4	Cytosol	细胞质基质	0.20000	`XP_057798343.1`
5	Cytoplasmic vesicle	细胞质囊泡	0.20000	`XP_057798344.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr3:53741235`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B115,B20,B68
`Chr3:53741449`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B69
`Chr3:53744362`	`GA`	`G`	1bp_deletion	frameshift_variant	1	A6
`Chr3:53744362`	`GA`	`G`	1bp_deletion	CDS_variant	1	A6
`Chr3:53744364`	`GC`	`G`	1bp_deletion	frameshift_variant	1	A6
`Chr3:53744364`	`GC`	`G`	1bp_deletion	CDS_variant	1	A6
`Chr3:53744405`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B38,B39
`Chr3:53746051`	`TG`	`T`	1bp_deletion	frameshift_variant	1	B12
`Chr3:53746051`	`TG`	`T`	1bp_deletion	CDS_variant	1	B12
`Chr3:53747670`	`C`	`CA`	1bp_insertion	frameshift_variant	19	A2,B11,B129,B131,B133,B17,B19,B31,B59,B65,B95,B99,C36,C39,C43,C51,C65,C75,D5
`Chr3:53747676`	`C`	`CA`	1bp_insertion	frameshift_variant	11	B104,B125,B127,B130,B138,B23,B45,B53,B57,B67,C46

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057798343.1

441 aa

220

441

Vps36_ESCRT-II

EAP30

PF11605 Vps36_ESCRT-II 10-102 aa PF04157 EAP30 172-395 aa

XP_057798344.1

435 aa

218

435

Vps36_ESCRT-II

EAP30

PF11605 Vps36_ESCRT-II 10-102 aa PF04157 EAP30 172-395 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057798343.1`	Pfam	`PF11605`	Vps36_ESCRT-II	Domain	10	102	6.5e-12	46.2	Vacuolar protein sorting protein 36 Vps36
`XP_057798343.1`	Pfam	`PF04157`	EAP30	Family	172	395	2.5e-57	194.7	EAP30/Vps36 family
`XP_057798344.1`	Pfam	`PF11605`	Vps36_ESCRT-II	Domain	10	102	6.4e-12	46.2	Vacuolar protein sorting protein 36 Vps36
`XP_057798344.1`	Pfam	`PF04157`	EAP30	Family	172	395	2.4e-57	194.7	EAP30/Vps36 family

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

2 records

下载 cDNA FASTA

XM_057942360.1
GCAAATAATCCTTTAGTTTACAGTTGACAATCAATCTCTCTTAGCTGTTTTCCCAGTTTCTTCAGTGAATATATACGATGGCCGGAGCCTTCCTTCCGTCGGCGGAGTTGACGGCCAGCGGCCGCCCCGTCTTGAAAGCCGATGAAGTAGAGTGCTCCCTCCTCTCCGCCGTTGACCTCCTCTCCGATGACACGCCCACCTTCCCTCACCTCAAGTCCGGCGTCGTCATCCTCACCACCCTCCGCCTCCTCTGGATCCCG...

XM_057942361.1
CAAATAATCCTTTAGTTTACAGTTGACAATCAATCTCTCTTAGCTGTTTTCCCAGTTTCTTCAGTGAATATATACGATGGCCGGAGCCTTCCTTCCGTCGGCGGAGTTGACGGCCAGCGGCCGCCCCGTCTTGAAAGCCGATGAAGTAGAGTGCTCCCTCCTCTCCGCCGTTGACCTCCTCTCCGATGACACGCCCACCTTCCCTCACCTCAAGTCCGGCGTCGTCATCCTCACCACCCTCCGCCTCCTCTGGATCCCGC...

CDS

2 records

下载 CDS FASTA

LOC131014404
ATGGCCGGAGCCTTCCTTCCGTCGGCGGAGTTGACGGCCAGCGGCCGCCCCGTCTTGAAAGCCGATGAAGTAGAGTGCTCCCTCCTCTCCGCCGTTGACCTCCTCTCCGATGACACGCCCACCTTCCCTCACCTCAAGTCCGGCGTCGTCATCCTCACCACCCTCCGCCTCCTCTGGATCCCGCCGGAAAACACTTCCTACGCAAGCACTTGTTGCTTCATCCCTCTTTCCGCAGTCCAAAACATACTCTCCGCCAAGAA...

Protein

2 records

下载 Protein FASTA

XP_057798343.1
MAGAFLPSAELTASGRPVLKADEVECSLLSAVDLLSDDTPTFPHLKSGVVILTTLRLLWIPPENTSYASTCCFIPLSAVQNILSAKKSIKSMFQSPRIRFQVAATPEGGVSEKGGSRSLVITLVVRGKTDPDSFLGKFWDAWRGRAWEASGSALREGEGSSSGSLAVRMPVVGLAGILRKEQEMWESTDKSLQDAFQDLNALMSKAKEMVMLAEKMRLKLLSGSNNQTSGAEDEEMGTKEMQDWLLSVGIVSPVTKESAG...

XP_057798344.1
MAGAFLPSAELTASGRPVLKADEVECSLLSAVDLLSDDTPTFPHLKSGVVILTTLRLLWIPPENTSYASTCCFIPLSAVQNILSAKKSIKSMFQSPRIRFQVAATPEGGVSEKGGSRSLVITLVVRGKTDPDSFLGKFWDAWRGRAWEASGSALREGEGSSSGSLAVRMPVVGLAGILRKEQEMWESTDKSLQDAFQDLNALMSKAKEMVMLAEKMRLKLLSGSNNQTSGAEDEEMGTKEMQDWLLSVGIVSPVTKESAG...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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