Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr03G004730`

染色体: Chr03 坐标: 64465734-64468897 链方向: + 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr03G004730`
名称	SmilChr03G004730
描述	cysteine--tRNA ligase, chloroplastic/mitochondrial-like
染色体 / SeqID	Chr03 / NC_080389.1
坐标与链方向	64465734 - 64468897 / `+`

ID 对照

NCBI Gene ID	`LOC131015566`
GeneID	`131015566`
locus_tag	`-`
NCBI Name	`LOC131015566`
原始查询	`LOC131015566`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	cysteine--tRNA ligase, chloroplastic/mitochondrial-like
产物	cysteine--tRNA ligase%2C chloroplastic/mitochondrial-like
Arabidopsis	`AT2G31170` (SYCO ARATH) Cysteinyl-tRNA synthetase, class Ia family protein [Source:NCBI gene (formerly Entrezgene);Acc:817673] identity=70.8; qcov=0.996; evalue=0; confidence=high
Rice	`Os09g0556500` - identity=71.5; qcov=0.875; evalue=0; confidence=high
GO	GO:0004817 (cysteine-tRNA ligase activity); GO:0005524 (ATP binding); GO:0005737 (cytoplasm); GO:0006423 (cysteinyl-tRNA aminoacylation)
KEGG	smil00970 (Aminoacyl-tRNA biosynthesis - Salvia miltiorrhiza (redroot sage))
Pfam	PF01406 (tRNA-synt_1e); PF09334 (tRNA-synt_1g); PF23493 (CysS_C)
InterPro	-
eggNOG	-
NR	GeneID:131015566

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Chloroplast	叶绿体	1.00000	`XP_057799975.1`
2	Chloroplast stroma	叶绿体基质	0.20000	`XP_057799975.1`
3	Nucleus	细胞核	0.00000	`XP_057799975.1`
4	Chromosome	染色体	0.00000	`XP_057799975.1`
5	Centromere	着丝粒	0.00000	`XP_057799975.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr3:64465857`	`G`	`A`	EMS_canonical_SNV	stop_gained	6	B139,B62,B73,C34,C75,D9
`Chr3:64465888`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	D11
`Chr3:64465905`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B104
`Chr3:64465945`	`CA`	`C`	1bp_deletion	frameshift_variant	18	A10,A2,A7,B11,B113,B122,B131,B40,B48,B58,B67,B74,B94,B97,C2,C31,C46,C51
`Chr3:64466014`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B49,C75
`Chr3:64466680`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B114,C37
`Chr3:64466812`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C58
`Chr3:64466818`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B65,C8
`Chr3:64466884`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B114
`Chr3:64467142`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B127
`Chr3:64467154`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	D11
`Chr3:64467259`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B83,C59

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057799975.1

566 aa

283

566

tRNA-synt_1e

tRNA-synt_1g

CysS_C

PF01406 tRNA-synt_1e 86-382 aa PF09334 tRNA-synt_1g 107-174 aa PF23493 CysS_C 518-556 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057799975.1`	Pfam	`PF01406`	tRNA-synt_1e	Domain	86	382	1.9e-129	432.1	tRNA synthetases class I (C) catalytic domain
`XP_057799975.1`	Pfam	`PF09334`	tRNA-synt_1g	Family	107	174	9.6e-12	45.1	tRNA synthetases class I (M)
`XP_057799975.1`	Pfam	`PF23493`	CysS_C	Domain	518	556	3e-06	28.0	Cysteinyl-tRNA ligase anticodon binding domain

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

1 records

下载 cDNA FASTA

XM_057943992.1
GAAACTAACTAATTAACAAACGCTCAAAACTTCTCTCTCACACACTACACTACACAATTTTCTGAACCCAACTGAAAGTCTAAAAGAGAGAGAGAGAGAGAGCTATGGCGACGATACTGAGATGGTACAAACCCTTGTTTCCGCTCCGCCACCGCAAACCTCTGGCATTCGCGTCGAAATCTTTCCATTGGAAGAGCAAATTCCGCCTTCGCAGCACAATCAATTGCAGCTACAACAAGTATCAATCCTTGAATACCTGC...

CDS

1 records

下载 CDS FASTA

LOC131015566
ATGGCGACGATACTGAGATGGTACAAACCCTTGTTTCCGCTCCGCCACCGCAAACCTCTGGCATTCGCGTCGAAATCTTTCCATTGGAAGAGCAAATTCCGCCTTCGCAGCACAATCAATTGCAGCTACAACAAGTATCAATCCTTGAATACCTGCTGTGAGGAAAAAATCGATAACAAAAATCGCGATGGTAACTCGCGGGAAGCGAAGAAAGAAGTCTGGCTGTACAATACGATGAGCAAGCAACGAGAGCTGTTTAA...

Protein

1 records

下载 Protein FASTA

XP_057799975.1
MATILRWYKPLFPLRHRKPLAFASKSFHWKSKFRLRSTINCSYNKYQSLNTCCEEKIDNKNRDGNSREAKKEVWLYNTMSKQRELFKPKVEGRVGMYVCGVTAYDLSHIGHARVYVAFDVLYRYLKHLGYEVNYVRNFTDVDDKIIARANELGEDPISLSRRYCEEFHHDMACLNCLPPSVEPRVSDHIPQIIAMIRQILDNECAYAIDGDVYFSIDKFPEYGRLSGRKLEDNRAGQRVAVDSRKRNTADFALWKAAKEG...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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JBrowse

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