Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr03G002834`

染色体: Chr03 坐标: 44856633-44860514 链方向: - 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr03G002834`
名称	SmilChr03G002834
描述	ent-kaurene oxidase
染色体 / SeqID	Chr03 / NC_080389.1
坐标与链方向	44856633 - 44860514 / `-`

ID 对照

NCBI Gene ID	`LOC131015983`
GeneID	`131015983`
locus_tag	`-`
NCBI Name	`LOC131015983`
原始查询	`LOC131015983`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	ent-kaurene oxidase
产物	ent-kaurene oxidase
Arabidopsis	`AT5G25900` (GA3) GA requiring 3 [Source:NCBI gene (formerly Entrezgene);Acc:832659] identity=64.5; qcov=0.742; evalue=1.65e-144; confidence=high
Rice	`Os06g0569900` (OsKO1) - identity=55.8; qcov=0.742; evalue=3.38e-131; confidence=high
GO	GO:0005506 (iron ion binding); GO:0005783 (endoplasmic reticulum); GO:0009686 (gibberellin biosynthetic process); GO:0009707 (chloroplast outer membrane); GO:0010241 (ent-kaurene oxidation to kaurenoic acid); GO:0020037 (heme binding); GO:0052615 (ent-kaurene oxidase activity)
KEGG	smil00904 (Diterpenoid biosynthesis - Salvia miltiorrhiza (redroot sage)); smil01100 (Metabolic pathways - Salvia miltiorrhiza (redroot sage)); smil01110 (Biosynthesis of secondary metabolites - Salvia miltiorrhiza (redroot sage))
Pfam	PF00067 (p450)
InterPro	-
eggNOG	-
NR	GeneID:131015983

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Endoplasmic reticulum	内质网	1.00000	`XP_057800496.1`
2	Endoplasmic reticulum membrane	内质网膜	1.00000	`XP_057800496.1`
3	Nucleus	细胞核	0.00000	`XP_057800496.1`
4	Chromosome	染色体	0.00000	`XP_057800496.1`
5	Centromere	着丝粒	0.00000	`XP_057800496.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr3:44856919`	`C`	`T`	EMS_canonical_SNV	CDS_variant	4	B123,B16,B35,C22
`Chr3:44856987`	`GTC`	`G`	2bp_deletion	frameshift_variant	25	A18,A23,B107,B139,B21,B28,B3,B46,B5,B53,B54,B62,B66,C18,C2,C23,C28,C59,C65,C74,C79,C81,C87,D2,D3
`Chr3:44857087`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B88,C22
`Chr3:44857088`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B88,C22
`Chr3:44857100`	`TC`	`T`	1bp_deletion	frameshift_variant	1	C22
`Chr3:44857100`	`TC`	`T`	1bp_deletion	CDS_variant	1	C22
`Chr3:44857116`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B6,C22
`Chr3:44857152`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B6
`Chr3:44857161`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B124,C22
`Chr3:44857171`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B124,B16,C22
`Chr3:44857184`	`G`	`A`	EMS_canonical_SNV	CDS_variant	3	B124,B8,B88
`Chr3:44857194`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B6,B8,C67

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057800496.1

519 aa

260

519

p450

PF00067 p450 48-492 aa

XP_057800497.1

418 aa

209

418

p450

PF00067 p450 48-113 aa PF00067 p450 140-391 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057800496.1`	Pfam	`PF00067`	p450	Domain	48	492	8.1e-71	239.9	Cytochrome P450
`XP_057800497.1`	Pfam	`PF00067`	p450	Domain	48	113	6.8e-65	220.3	Cytochrome P450
`XP_057800497.1`	Pfam	`PF00067`	p450	Domain	140	391	6.8e-65	220.3	Cytochrome P450

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

2 records

下载 cDNA FASTA

XM_057944513.1
AAGGATAAGGAACCACATGCTATATATCTATCTCCATCACAGAACCCACTTCAATTTCTTTATACTCCACCAAACATCTCCGTCATTCTCCACCAATCCACCTAACGTACTCATTATACCACAAAGCTTTCCACCCTATACCACTTTAAATTAACCATGATTAAAATGGCTGTCTTGTCTCCCAACTCCTCTCTCAATTTCAATTCAAATCCTAATTGTATTTGAAGGTTACTCTTTCACTACCATTTCAACCTCAAACA...

XM_057944514.1
AACCCACTTCAATTTCTTTATACTCCACCAAACATCTCCGTCATTCTCCACCAATCCACCTAACGTACTCATTATACCACAAAGCTTTCCACCCTATACCACTTTAAATTAACCATGATTAAAATGGCTGTCTTGTCTCCCAACTCCTCTCTCAATTTCAATTCAAATCCTAATTGTATTTGAAGGTTACTCTTTCACTACCATTTCAACCTCAAACAAGAAAAAGAAACCATGTGCAGAGCCTTTCAATCAGAGGTTTA...

CDS

2 records

下载 CDS FASTA

LOC131015983
ATGGATACACTGCTGAGTCTTCAAGCTGTGCCGGCAGCCGCCGCTATCGGCGGACCTGTGGTGGCTATAGGTGGAATCACTTTGTTTTTCATCAGAGAATATGTCAAGGATCAAAGGAAAAAATCTTCCAGCTTCCCTCCACCGCCTAAGGTACCAGGCTTACCAGTAATTGGAAATTTGTTGCAATTAAAAGAGAAGAAGCCCCACAAGACTTTCACCAAATGGTCTGAGAAATATGGTCCAATATATTCTATAAGGAC...

Protein

2 records

下载 Protein FASTA

XP_057800496.1
MDTLLSLQAVPAAAAIGGPVVAIGGITLFFIREYVKDQRKKSSSFPPPPKVPGLPVIGNLLQLKEKKPHKTFTKWSEKYGPIYSIRTGANTMIVLNTNDVAKEAMVTKYSSISTRKLSKALTILTSDKSIVAMSDYNEFYKAAKRHLLTSTLGPTAQKRHRVHRNLMINNICDQFLAHAKMYPSEAVNFRKIFQSELFGLSMKQAIGEDVESIYVEDLDTTLSRQEMFKILVVDPMEGAIDVDWRDFFPYLKWIPNQHFE...

XP_057800497.1
MDTLLSLQAVPAAAAIGGPVVAIGGITLFFIREYVKDQRKKSSSFPPPPKVPGLPVIGNLLQLKEKKPHKTFTKWSEKYGPIYSIRTGANTMIVLNTNDVAKEAIGEDVESIYVEDLDTTLSRQEMFKILVVDPMEGAIDVDWRDFFPYLKWIPNQHFENKIQQMHFHREAVMKALIEQQKKRIASGKAINCYLDHLLSEAADTLSEQQILMLLWEAIIEASDTTLVTTEWAMYELSKDPKRQNYLLSEIQNACGFDQLN...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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