Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr01G001302`

染色体: Chr01 坐标: 25929596-25933565 链方向: - 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr01G001302`
名称	SmilChr01G001302
描述	isoleucine--tRNA ligase, cytoplasmic
染色体 / SeqID	Chr01 / NC_080387.1
坐标与链方向	25929596 - 25933565 / `-`

ID 对照

NCBI Gene ID	`LOC131016869`
GeneID	`131016869`
locus_tag	`-`
NCBI Name	`LOC131016869`
原始查询	`LOC131016869`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	isoleucine--tRNA ligase, cytoplasmic
产物	isoleucine--tRNA ligase%2C cytoplasmic
Arabidopsis	`AT4G10320` (AT4G10320) tRNA synthetase class I (I, L, M and V) family protein [Source:NCBI gene (formerly Entrezgene);Acc:826624] identity=75.5; qcov=1.01; evalue=0; confidence=high
Rice	`Os06g0645400` (P0017B12.25-1) - identity=76.3; qcov=0.675; evalue=0; confidence=high
GO	GO:0000049 (tRNA binding); GO:0002161 (aminoacyl-tRNA deacylase activity); GO:0004822 (isoleucine-tRNA ligase activity); GO:0005524 (ATP binding); GO:0006428 (isoleucyl-tRNA aminoacylation)
KEGG	smil00970 (Aminoacyl-tRNA biosynthesis - Salvia miltiorrhiza (redroot sage))
Pfam	PF00133 (tRNA-synt_1); PF08264 (Anticodon_1); PF09334 (tRNA-synt_1g); PF19302 (DUF5915)
InterPro	-
eggNOG	-
NR	GeneID:131016869

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Cytosol	细胞质基质	1.00000	`XP_057801635.1`
2	Cytoplasm	细胞质	0.80000	`XP_057801635.1`
3	Nucleus	细胞核	0.00000	`XP_057801635.1`
4	Chromosome	染色体	0.00000	`XP_057801635.1`
5	Centromere	着丝粒	0.00000	`XP_057801635.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr1:25929965`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B138
`Chr1:25929983`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B103
`Chr1:25930210`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C86
`Chr1:25930258`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B124
`Chr1:25930440`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	D11
`Chr1:25930570`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C79
`Chr1:25930760`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B72
`Chr1:25930814`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C9
`Chr1:25931310`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	C11,C69
`Chr1:25931552`	`TC`	`T`	1bp_deletion	frameshift_variant	1	B87
`Chr1:25931552`	`TC`	`T`	1bp_deletion	CDS_variant	1	B87
`Chr1:25932002`	`G`	`A`	EMS_canonical_SNV	CDS_variant	4	B109,B34,B53,B83

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057801635.1

1177 aa

588

1177

tRNA-synt_1

tRNA-synt_1g

Anticodon_1

DUF5915

PF00133 tRNA-synt_1 18-663 aa PF09334 tRNA-synt_1g 49-109 aa PF08264 Anticodon_1 721-870 aa PF19302 DUF5915 1003-1057 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057801635.1`	Pfam	`PF00133`	tRNA-synt_1	Domain	18	663	2.4e-161	537.8	tRNA synthetases class I (I, L, M and V)
`XP_057801635.1`	Pfam	`PF09334`	tRNA-synt_1g	Family	49	109	2.7e-15	56.8	tRNA synthetases class I (M)
`XP_057801635.1`	Pfam	`PF08264`	Anticodon_1	Domain	721	870	1.2e-28	100.6	Anticodon-binding domain of tRNA ligase
`XP_057801635.1`	Pfam	`PF19302`	DUF5915	Domain	1003	1057	1.1e-06	29.4	Domain of unknown function (DUF5915)

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

1 records

下载 cDNA FASTA

XM_057945652.1
TACCTACGTGGCGGCCGACCATTTTAGAGAAAGGGCGCTGCCAAAGTAACGCCATTTCAACGCAAAACACAAAATCCTCGCCACTCCAAAACCCTAGACACTGTTACCCGGCGGCGCAGCGGCGGCGATCAATCATCAATGGAGGACGTCTGCGAGGGAAAGGACTTCTCGTTCCCCTCCCAGGAAGAGAAGATCCTCTCATGGTGGGACGAAATCGACGCCTTCAAAACCCAACTCGACCGCACCAAGCATTTCCCGGA...

CDS

1 records

下载 CDS FASTA

LOC131016869
ATGGAGGACGTCTGCGAGGGAAAGGACTTCTCGTTCCCCTCCCAGGAAGAGAAGATCCTCTCATGGTGGGACGAAATCGACGCCTTCAAAACCCAACTCGACCGCACCAAGCATTTCCCGGAGTACATCTTCTATGACGGCCCGCCCTTCGCCACCGGCCTCCCCCACTACGGTCACATTCTCGCCGGAACTATCAAGGACATTGTTACTCGCTATCAGTCGATGACGGGGCATCACGTCACCCGAAGATTCGGCTGGGA...

Protein

1 records

下载 Protein FASTA

XP_057801635.1
MEDVCEGKDFSFPSQEEKILSWWDEIDAFKTQLDRTKHFPEYIFYDGPPFATGLPHYGHILAGTIKDIVTRYQSMTGHHVTRRFGWDCHGLPVEHEIDQKLGIKSREDVFKMGIANYNEECRGIVQRYVGEWEKTVVRMGRWIDFRNDYKTMDLNFMESVWWVFAQLFQKGLVYRGFKVMPYSTGCKTPLSNFEANSNYKEVPDPEIMVAFPVVDDADGASFVAWTTTPWTLPSNLALCVNAGFAYVKVRNKFNGKVFVV...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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JBrowse

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