Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr01G001390`

染色体: Chr01 坐标: 27323002-27328058 链方向: + 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr01G001390`
名称	SmilChr01G001390
描述	calcium-dependent protein kinase 8-like
染色体 / SeqID	Chr01 / NC_080387.1
坐标与链方向	27323002 - 27328058 / `+`

ID 对照

NCBI Gene ID	`LOC131017744`
GeneID	`131017744`
locus_tag	`-`
NCBI Name	`LOC131017744`
原始查询	`LOC131017744`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	calcium-dependent protein kinase 8-like
产物	calcium-dependent protein kinase 8-like
Arabidopsis	`AT5G19450` (CDPK19) calcium-dependent protein kinase 19 [Source:NCBI gene (formerly Entrezgene);Acc:832065] identity=82.3; qcov=0.998; evalue=0; confidence=high
Rice	`Os07g0568600` (OsCDPK20) - identity=74.3; qcov=1.03; evalue=0; confidence=high
GO	GO:0004683 (calcium/calmodulin-dependent protein kinase activity); GO:0005509 (calcium ion binding); GO:0005516 (calmodulin binding); GO:0005524 (ATP binding); GO:0005634 (nucleus); GO:0005737 (cytoplasm); GO:0009931 (calcium-dependent protein serine/threonine kinase activity); GO:0018105 (peptidyl-serine phosphorylation); GO:0035556 (intracellular signal transduction); GO:0046777 (protein autophosphorylation)
KEGG	smil04075 (Plant hormone signal transduction - Salvia miltiorrhiza (redroot sage)); smil04626 (Plant-pathogen interaction - Salvia miltiorrhiza (redroot sage))
Pfam	PF00069 (Pkinase); PF07714 (PK_Tyr_Ser-Thr); PF13202 (EF-hand_5); PF13405 (EF-hand_6); PF13499 (EF-hand_7); PF13833 (EF-hand_8)
InterPro	-
eggNOG	-
NR	GeneID:131017744

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Cell membrane	细胞膜	1.00000	`XP_057802449.1`
2	Cytosol	细胞质基质	0.40000	`XP_057802449.1`
3	Cytoplasm	细胞质	0.20000	`XP_057802449.1`
4	Nucleus	细胞核	0.00000	`XP_057802449.1`
5	Chromosome	染色体	0.00000	`XP_057802449.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr1:27323758`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	C10,C79
`Chr1:27323818`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B117
`Chr1:27323863`	`G`	`A`	EMS_canonical_SNV	CDS_variant	3	C10,C79,D11
`Chr1:27323893`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	A23
`Chr1:27323948`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C64
`Chr1:27326439`	`TC`	`T`	1bp_deletion	frameshift_variant	1	C71
`Chr1:27326439`	`TC`	`T`	1bp_deletion	CDS_variant	1	C71
`Chr1:27327010`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	C10,C79
`Chr1:27327077`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B50,C40
`Chr1:27327653`	`G`	`GAT`	2bp_insertion	frameshift_variant	10	B121,B33,B47,B91,B94,C3,C49,C61,C7,D2

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057802449.1

533 aa

266

533

Pkinase

PK_Tyr_Ser-Thr

EF-hand_7

EF-hand_8

EF-hand_5

EF-hand_7

EF-hand_6

EF-hand_5

EF-hand_8

EF-hand_5

PF00069 Pkinase 57-315 aa PF07714 PK_Tyr_Ser-Thr 59-311 aa PF13499 EF-hand_7 363-422 aa PF13833 EF-hand_8 375-425 aa PF13202 EF-hand_5 402-421 aa PF13499 EF-hand_7 433-496 aa PF13405 EF-hand_6 435-460 aa PF13202 EF-hand_5 437-457 aa PF13833 EF-hand_8 446-496 aa PF13202 EF-hand_5 473-495 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057802449.1`	Pfam	`PF00069`	Pkinase	Domain	57	315	9.5e-80	268.5	Protein kinase domain
`XP_057802449.1`	Pfam	`PF07714`	PK_Tyr_Ser-Thr	Domain	59	311	2.2e-25	90.1	Protein tyrosine and serine/threonine kinase
`XP_057802449.1`	Pfam	`PF13499`	EF-hand_7	Domain	363	422	5.8e-30	104.2	EF-hand domain pair
`XP_057802449.1`	Pfam	`PF13833`	EF-hand_8	Domain	375	425	1.6e-12	47.7	EF-hand domain pair
`XP_057802449.1`	Pfam	`PF13202`	EF-hand_5	Domain	402	421	1.3e-15	56.7	EF hand
`XP_057802449.1`	Pfam	`PF13499`	EF-hand_7	Domain	433	496	5.8e-30	104.2	EF-hand domain pair
`XP_057802449.1`	Pfam	`PF13405`	EF-hand_6	Domain	435	460	1.6e-19	68.4	EF-hand domain
`XP_057802449.1`	Pfam	`PF13202`	EF-hand_5	Domain	437	457	1.3e-15	56.7	EF hand
`XP_057802449.1`	Pfam	`PF13833`	EF-hand_8	Domain	446	496	1.6e-12	47.7	EF-hand domain pair
`XP_057802449.1`	Pfam	`PF13202`	EF-hand_5	Domain	473	495	1.3e-15	56.7	EF hand

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

1 records

下载 cDNA FASTA

XM_057946466.1
TGGCTTTGCCTAACAGCCAAACACAACAAGTGGCATGCGGGTACCGGAAACCACGCATATATATTAATTCAAGAAAGCTTAGTGAGATAAAGAGAAGTCACAGAAGGGGGAACGGGGGAATTCAGGAGAGAGAAACTCAGAAAAGTCTCTGCACAAGAGCTTTTTGACTCTCTCTTTCTCTATTCCCATGGCGGACGATCTCCTTCATGTGTCAATCCTTTCACATTTCGTGTTTTTGGTTTTCTCGGTTGAGTTTTGAC...

CDS

1 records

下载 CDS FASTA

LOC131017744
ATGGGAAACTGCTGCGTGACTCCTTATAGTCCGTCTGAAAAGAAGAAGAAGAAACACAAGAATAAGCCGAATCCATTTTCCGCAGATTATGGTGTGAGTCGTGGATCTTGGGGAACAAACAAGCTCGTCGTGTTGAAGGATCCAGGAGGCCATAACATCAAGGAGAGATATGATCTCGGGCGTGAGCTCGGACGAGGCGAATTTGGGATTACCTATTTGTGTACTGATGTGGAGACAGGGGAGAAATACGCGTGCAAGTC...

Protein

1 records

下载 Protein FASTA

XP_057802449.1
MGNCCVTPYSPSEKKKKKHKNKPNPFSADYGVSRGSWGTNKLVVLKDPGGHNIKERYDLGRELGRGEFGITYLCTDVETGEKYACKSISKKKLRTAVDIEDVRREVEIMKHMPKHLNIVSLKDTYEDDNAVHIVMELCEGGELFDRIVARGHYTERAAAVVMKTIVEVVQVCHQHGVMHRDLKPENFLFANKKETSPVKAIDFGLSVFFRPGERFNEIVGSPYYMAPEVLKRNYGPEIDIWSAGIILYILLCGVPPFWAE...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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JBrowse

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