当前解析结果
SmilChr01G001791| 统一新 Gene ID | SmilChr01G001791 |
|---|---|
| 名称 | SmilChr01G001791 |
| 描述 | phenylalanine--tRNA ligase beta subunit, cytoplasmic |
| 染色体 / SeqID | Chr01 / NC_080387.1 |
| 坐标与链方向 | 32771175 - 32776784 / + |
| NCBI Gene ID | LOC131020301 |
|---|---|
| GeneID | 131020301 |
| locus_tag | - |
| NCBI Name | LOC131020301 |
| 原始查询 | LOC131020301 |
| 来源 | GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog |
|---|---|
| 描述 | phenylalanine--tRNA ligase beta subunit, cytoplasmic |
| 产物 | phenylalanine--tRNA ligase beta subunit%2C cytoplasmic |
| Arabidopsis | AT1G72550 (AT1G72550)tRNA synthetase beta subunit family protein [Source:NCBI gene (formerly Entrezgene);Acc:843587] identity=71.3; qcov=1.01; evalue=0; confidence=high |
| Rice | Os05g0558900 (PAL11)- identity=66.8; qcov=1.02; evalue=0; confidence=high |
| GO | GO:0000287 (magnesium ion binding); GO:0003723 (RNA binding); GO:0004826 (phenylalanine-tRNA ligase activity); GO:0005524 (ATP binding); GO:0005737 (cytoplasm); GO:0006432 (phenylalanyl-tRNA aminoacylation); GO:0009328 (phenylalanine-tRNA ligase complex) |
| KEGG | smil00970 (Aminoacyl-tRNA biosynthesis - Salvia miltiorrhiza (redroot sage)) |
| Pfam | PF03483 (B3_4); PF03484 (B5); PF17759 (tRNA_synthFbeta); PF18262 (PhetRS_B1) |
| InterPro | - |
| eggNOG | - |
| NR | GeneID:131020301 |
| Rank | 定位位置 | 中文 | Score | Protein ID |
|---|---|---|---|---|
| 1 | Cytoplasm | 细胞质 | 1.00000 | XP_057805015.1 |
| 2 | Cytosol | 细胞质基质 | 1.00000 | XP_057805015.1 |
| 3 | Nucleoplasm | 核质 | 0.20000 | XP_057805015.1 |
| 4 | Mitochondrion matrix | 线粒体基质 | 0.20000 | XP_057805015.1 |
| 5 | Nucleus | 细胞核 | 0.00000 | XP_057805015.1 |
下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。
| 位置 | REF | ALT | 类型 | 影响 | 携带材料数 | 材料 |
|---|---|---|---|---|---|---|
Chr1:32771288 |
G |
A |
EMS_canonical_SNV | CDS_variant | 5 | A10,B27,B40,B8,C58 |
Chr1:32771304 |
AGC |
A |
2bp_deletion | frameshift_variant | 1 | B114 |
Chr1:32771304 |
AGC |
A |
2bp_deletion | CDS_variant | 1 | B114 |
Chr1:32771408 |
CT |
C |
1bp_deletion | frameshift_variant | 44 | A15,A21,A23,A25,B109,B114,B121,B122,B126,B127,B137,B17,B19,B28,B35,B46,B47,B54,B56,B57,B58,B69,B70,B71,B73,B80,B84,B96,B98,C2,C21,C22,C29,C31,C48,C50,C65,C7,C71,C76,C82,C83,D3,D4 |
Chr1:32771411 |
A |
AT |
1bp_insertion | frameshift_variant | 81 | A15,A21,A23,A25,A3,B102,B106,B109,B113,B114,B119,B121,B122,B123,B126,B127,B137,B16,B17,B19,B27,B28,B3,B35,B40,B44,B45,B46,B47,B54,B56,B57,B58,B69,B70,B71,B73,B78,B8,B80,B83,B84,B85,B86,B96,B97,B98,C1,C12,C15,C2,C21,C22,C24,C29,C31,C32,C35,C38,C44,C45,C48,C50,C53,C54,C58,C59,C61,C65,C68,C7,C71,C72,C73,C76,C79,C82,C83,C87,D3,D4 |
Chr1:32771620 |
G |
A |
EMS_canonical_SNV | CDS_variant | 2 | B7,C45 |
Chr1:32772167 |
G |
A |
EMS_canonical_SNV | CDS_variant | 1 | C75 |
Chr1:32772869 |
C |
T |
EMS_canonical_SNV | CDS_variant | 1 | C83 |
Chr1:32773322 |
G |
A |
EMS_canonical_SNV | CDS_variant | 5 | A10,B27,B40,B8,C58 |
Chr1:32773805 |
G |
A |
EMS_canonical_SNV | CDS_variant | 5 | A10,B27,B40,B8,C58 |
Chr1:32773849 |
TA |
T |
1bp_deletion | frameshift_variant | 1 | B68 |
Chr1:32773849 |
TA |
T |
1bp_deletion | CDS_variant | 1 | B68 |
结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。
XP_057805015.1PF18262
PhetRS_B1 1-98 aa
PF03483
B3_4 146-295 aa
PF03484
B5 317-387 aa
PF17759
tRNA_synthFbeta 390-596 aa
XP_057805023.1PF18262
PhetRS_B1 1-90 aa
PF03483
B3_4 138-287 aa
PF03484
B5 309-379 aa
PF17759
tRNA_synthFbeta 382-588 aa
| Protein ID | 来源 | 结构域编号 | 结构域名称 | 类型 | 起始 aa | 终止 aa | E-value | Score | 说明 |
|---|---|---|---|---|---|---|---|---|---|
XP_057805015.1 |
Pfam | PF18262 |
PhetRS_B1 | Domain | 1 | 98 | 1.2e-27 | 96.7 | Phe-tRNA synthetase beta subunit B1 domain |
XP_057805015.1 |
Pfam | PF03483 |
B3_4 | Domain | 146 | 295 | 1.8e-14 | 54.4 | B3/4 domain |
XP_057805015.1 |
Pfam | PF03484 |
B5 | Domain | 317 | 387 | 2.8e-22 | 79.3 | tRNA synthetase B5 domain |
XP_057805015.1 |
Pfam | PF17759 |
tRNA_synthFbeta | Domain | 390 | 596 | 1.6e-47 | 162.5 | Phenylalanyl tRNA synthetase beta chain CLM domain |
XP_057805023.1 |
Pfam | PF18262 |
PhetRS_B1 | Domain | 1 | 90 | 1.5e-32 | 112.4 | Phe-tRNA synthetase beta subunit B1 domain |
XP_057805023.1 |
Pfam | PF03483 |
B3_4 | Domain | 138 | 287 | 1.8e-14 | 54.4 | B3/4 domain |
XP_057805023.1 |
Pfam | PF03484 |
B5 | Domain | 309 | 379 | 7.1e-23 | 81.3 | tRNA synthetase B5 domain |
XP_057805023.1 |
Pfam | PF17759 |
tRNA_synthFbeta | Domain | 382 | 588 | 1.5e-47 | 162.5 | Phenylalanyl tRNA synthetase beta chain CLM domain |
每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。
2 records
下载 cDNA FASTA2 records
下载 CDS FASTA2 records
下载 Protein FASTAupstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。