Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr04G003428`

染色体: Chr04 坐标: 50865961-50869419 链方向: - 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr04G003428`
名称	SmilChr04G003428
描述	replication protein A 70 kDa DNA-binding subunit B
染色体 / SeqID	Chr04 / NC_080390.1
坐标与链方向	50865961 - 50869419 / `-`

ID 对照

NCBI Gene ID	`LOC131020320`
GeneID	`131020320`
locus_tag	`-`
NCBI Name	`LOC131020320`
原始查询	`LOC131020320`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	replication protein A 70 kDa DNA-binding subunit B
产物	replication protein A 70 kDa DNA-binding subunit B
Arabidopsis	`AT5G08020` (RPA70B) RPA70-kDa subunit B [Source:NCBI gene (formerly Entrezgene);Acc:830696] identity=70.1; qcov=0.989; evalue=0; confidence=high
Rice	`Os03g0214100` (OsRPA1) - identity=65.5; qcov=0.998; evalue=0; confidence=high
GO	GO:0000724 (double-strand break repair via homologous recombination); GO:0003684 (damaged DNA binding); GO:0005634 (nucleus); GO:0005662 (DNA replication factor A complex); GO:0006260 (DNA replication); GO:0006268 (GO:0006268); GO:0006289 (nucleotide-excision repair); GO:0007004 (telomere maintenance via telomerase); GO:0043047 (single-stranded telomeric DNA binding); GO:0051321 (meiotic cell cycle)
KEGG	smil03030 (DNA replication - Salvia miltiorrhiza (redroot sage)); smil03420 (Nucleotide excision repair - Salvia miltiorrhiza (redroot sage)); smil03430 (Mismatch repair - Salvia miltiorrhiza (redroot sage)); smil03440 (Homologous recombination - Salvia miltiorrhiza (redroot sage)); smil03460 (Fanconi anemia pathway - Salvia miltiorrhiza (redroot sage))
Pfam	PF01336 (tRNA_anti-codon); PF02721 (DUF223); PF04057 (Rep-A_N); PF08646 (Rep_fac-A_C); PF16900 (REPA_OB_2)
InterPro	-
eggNOG	-
NR	GeneID:131020320

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Nucleus	细胞核	1.00000	`XP_057805039.1`
2	Chromosome	染色体	1.00000	`XP_057805039.1`
3	Telomere	端粒	0.20000	`XP_057805039.1`
4	PML body	PML body	0.20000	`XP_057805039.1`
5	Nucleoplasm	核质	0.20000	`XP_057805039.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr4:50866693`	`C`	`T`	EMS_canonical_SNV	CDS_variant	4	A6,B76,C16,D5
`Chr4:50866980`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B115
`Chr4:50867571`	`G`	`A`	EMS_canonical_SNV	CDS_variant	4	B33,B4,C19,C71
`Chr4:50867814`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B121
`Chr4:50868162`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C70
`Chr4:50868169`	`G`	`A`	EMS_canonical_SNV	CDS_variant	4	A6,B76,C16,D5
`Chr4:50868395`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B21,D10
`Chr4:50869171`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C38
`Chr4:50869238`	`A`	`AT`	1bp_insertion	frameshift_variant	128	A13,A18,A19,A2,A20,A22,A23,A24,A25,A4,A6,A7,A8,B10,B100,B102,B103,B105,B107,B109,B11,B110,B111,B113,B115,B117,B119,B120,B122,B124,B125,B129,B135,B137,B138,B15,B18,B2,B22,B24,B25,B27,B28,B29,B30,B31,B33,B35,B36,B38,B39,B4,B40,B44,B45,B46,B47,B49,B5,B51,B6,B71,B72,B73,B75,B76,B8,B83,B84,B86,B89,B90,B92,B94,B97,B99,C12,C14,C15,C16,C19,C20,C21,C23,C25,C26,C27,C29,C31,C32,C35,C38,C4,C40,C47,C49,C5,C52,C54,C55,C57,C59,C60,C61,C63,C65,C67,C68,C71,C73,C74,C75,C77,C78,C8,C81,C82,C83,C86,C87,C9,D1,D10,D3,D5,D6,D7,D9

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057805039.1

537 aa

268

537

Rep-A_N

DUF223

tRNA_anti-codon

REPA_OB_2

Rep_fac-A_C

PF04057 Rep-A_N 5-103 aa PF02721 DUF223 181-300 aa PF01336 tRNA_anti-codon 189-274 aa PF16900 REPA_OB_2 308-402 aa PF08646 Rep_fac-A_C 466-535 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057805039.1`	Pfam	`PF04057`	Rep-A_N	Domain	5	103	5e-20	72.1	Replication factor-A protein 1, N-terminal domain
`XP_057805039.1`	Pfam	`PF02721`	DUF223	Domain	181	300	3.8e-11	43.9	Domain of unknown function DUF223
`XP_057805039.1`	Pfam	`PF01336`	tRNA_anti-codon	Domain	189	274	1.5e-10	41.6	OB-fold nucleic acid binding domain
`XP_057805039.1`	Pfam	`PF16900`	REPA_OB_2	Domain	308	402	1.1e-24	87.0	Replication protein A OB domain
`XP_057805039.1`	Pfam	`PF08646`	Rep_fac-A_C	Domain	466	535	1.9e-20	73.9	Replication factor-A C terminal domain

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

2 records

下载 cDNA FASTA

XR_009100674.1
TTCCCGCTTTACTTCCCAAAAATTTGCGCGCCCGTTTTCAAGATGTACAAATTCCTCATTATCAATCGCTTCTCCCACTTCAATCTTCACGTCCCTCTCTAGCTCTCCAACCAAAGCAACATCTGAGATCTGCTTCACCACCTACCTCATTTGACTGTGAAGTATAGTGAGAGAGCAGAAATGGCTAAAACTGTAAGCCCAGATGCTATATCGTTTATTCTAGCAAACCCATGCCCCGATTCTTCCACCGATTTGCCGGA...

XM_057949056.1
TTCCCGCTTTACTTCCCAAAAATTTGCGCGCCCGTTTTCAAGATGTACAAATTCCTCATTATCAATCGCTTCTCCCACTTCAATCTTCACGTCCCTCTCTAGCTCTCCAACCAAAGCAACATCTGAGATCTGCTTCACCACCTACCTCATTTGACTGTGAAGTATAGTGAGAGAGCAGAAATGGCTAAAACTGTAAGCCCAGATGCTATATCGTTTATTCTAGCAAACCCATGCCCCGATTCTTCCACCGATTTGCCGGA...

CDS

1 records

下载 CDS FASTA

LOC131020320
ATGGCTAAAACTGTAAGCCCAGATGCTATATCGTTTATTCTAGCAAACCCATGCCCCGATTCTTCCACCGATTTGCCGGAACTCATTGTACAAGTCCTCGATCTCAAGCCCACCGGCAACAAATACATGTTCATGGCCAGTGATGGCAAAATGAAGGTGAGGGCTCTGTTGCAGTCAACTCTAGCGTCTGAGGTGACATCTGGAAACATCCAGAATTTGGGGCTTCTTCGTATTCTTGACTACACTCTCAATGACATCGC...

Protein

1 records

下载 Protein FASTA

XP_057805039.1
MAKTVSPDAISFILANPCPDSSTDLPELIVQVLDLKPTGNKYMFMASDGKMKVRALLQSTLASEVTSGNIQNLGLLRILDYTLNDIATKNEKFLIVSKCNAVSPALESEYKTEVKVEGSGLSLKPHQAMDVDVKTESAGIVLKPKHEVMTKSAAQIVHDQHGNMAPAARMAMTRRVHPLVSLNPYQGNWTIKVRVTSKGNMRTYKNARGEGCVFNVELTDEDGTQIQATMFNDAARKFFDKFQMGKVYYISKGTLKVANK...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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