Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr04G003738`

染色体: Chr04 坐标: 53699217-53702767 链方向: - 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr04G003738`
名称	SmilChr04G003738
描述	malate dehydrogenase, cytoplasmic-like
染色体 / SeqID	Chr04 / NC_080390.1
坐标与链方向	53699217 - 53702767 / `-`

ID 对照

NCBI Gene ID	`LOC131020616`
GeneID	`131020616`
locus_tag	`-`
NCBI Name	`LOC131020616`
原始查询	`LOC131020616`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	malate dehydrogenase, cytoplasmic-like
产物	malate dehydrogenase%2C cytoplasmic-like
Arabidopsis	`AT1G04410` (c-NAD-MDH1) Lactate/malate dehydrogenase family protein [Source:NCBI gene (formerly Entrezgene);Acc:839527] identity=78.3; qcov=0.917; evalue=0; confidence=high
Rice	`Os10g0478200` (OscMDH) - identity=78; qcov=0.917; evalue=0; confidence=high
GO	GO:0006099 (tricarboxylic acid cycle); GO:0006107 (oxaloacetate metabolic process); GO:0006108 (malate metabolic process); GO:0006734 (GO:0006734); GO:0030060 (L-malate dehydrogenase (NAD+) activity)
KEGG	smil00020 (Citrate cycle (TCA cycle) - Salvia miltiorrhiza (redroot sage)); smil00270 (Cysteine and methionine metabolism - Salvia miltiorrhiza (redroot sage)); smil00566 (Sulfoquinovose metabolism - Salvia miltiorrhiza (redroot sage)); smil00620 (Pyruvate metabolism - Salvia miltiorrhiza (redroot sage)); smil00630 (Glyoxylate and dicarboxylate metabolism - Salvia miltiorrhiza (redroot sage)); smil00710 (Carbon fixation by Calvin cycle - Salvia miltiorrhiza (redroot sage)); smil01100 (Metabolic pathways - Salvia miltiorrhiza (redroot sage)); smil01110 (Biosynthesis of secondary metabolites - Salvia miltiorrhiza (redroot sage)); smil01200 (Carbon metabolism - Salvia miltiorrhiza (redroot sage))
Pfam	PF00056 (Ldh_1_N); PF02866 (Ldh_1_C)
InterPro	-
eggNOG	-
NR	GeneID:131020616

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Cytosol	细胞质基质	0.80000	`XP_057805523.1`
2	Cytoplasm	细胞质	0.40000	`XP_057805523.1`
3	Mitochondrion	线粒体	0.20000	`XP_057805523.1`
4	Mitochondrion membrane	线粒体膜	0.20000	`XP_057805523.1`
5	Lipid droplet	脂滴	0.20000	`XP_057805523.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr4:53699777`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B44
`Chr4:53699874`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B28
`Chr4:53699899`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B140
`Chr4:53701584`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C23
`Chr4:53701729`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B93,C8
`Chr4:53701759`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B41,B75
`Chr4:53701962`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B35,D11
`Chr4:53702623`	`AT`	`A`	1bp_deletion	frameshift_variant	6	B44,B48,B60,B98,C16,C60

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057805523.1

362 aa

181

362

Ldh_1_N

Ldh_1_C

PF00056 Ldh_1_N 40-183 aa PF02866 Ldh_1_C 187-355 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057805523.1`	Pfam	`PF00056`	Ldh_1_N	Domain	40	183	8.4e-31	107.6	lactate/malate dehydrogenase, NAD binding domain
`XP_057805523.1`	Pfam	`PF02866`	Ldh_1_C	Domain	187	355	4.6e-45	154.5	lactate/malate dehydrogenase, alpha/beta C-terminal domain

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

1 records

下载 cDNA FASTA

XM_057949540.1
ATCTCAAAAAAAATATATTCCAAGCCCACCATCCAGTTTTTGGGTCCTTAAATTTCCTATTTATATACTGTTGGAGAATTGCTGCAAAATTGAAAAAAGTGTATTTTTATTTATCCTATTTCTAATTCGAGAGGAAATTAAAAATGGAACAAATTTTGTCATTAGAAACCCCTCTTGTTGTTATGGGCTGTGTGCTCTTGTCTTGGAAAATTGTGAGACACATTTGGAGTTTTTTGGATGTTGGGAGAGAACCTCTCACT...

CDS

1 records

下载 CDS FASTA

LOC131020616
ATGGAACAAATTTTGTCATTAGAAACCCCTCTTGTTGTTATGGGCTGTGTGCTCTTGTCTTGGAAAATTGTGAGACACATTTGGAGTTTTTTGGATGTTGGGAGAGAACCTCTCACTGTTCTTGTTACTGGCGCTGCAGGTCAGATAGGTTATGCTCTAGTTCCGATGATAGCAAGAGGAGAAATGCTAGGTCCTCAACAACCTGTGATCATCCACATGCTCGACATCAACGCCGAGTCTCTCAACGCCGTCAAAATGGA...

Protein

1 records

下载 Protein FASTA

XP_057805523.1
MEQILSLETPLVVMGCVLLSWKIVRHIWSFLDVGREPLTVLVTGAAGQIGYALVPMIARGEMLGPQQPVIIHMLDINAESLNAVKMELIDSALPLLRGVVATTNVAEACSGVNVAVLVGGSPRKEGMERKDVMSRNVSIYKAHASALEQHAAPDCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRAVAQIAERLEVEVGDVKNVIVWGNHSSSQYPDATHAAVKTIAGERSVRELVADDKWLRGELVERVQQ...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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JBrowse

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