Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr01G002001`

染色体: Chr01 坐标: 35519156-35526979 链方向: + 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr01G002001`
名称	SmilChr01G002001
描述	replication factor C subunit 1
染色体 / SeqID	Chr01 / NC_080387.1
坐标与链方向	35519156 - 35526979 / `+`

ID 对照

NCBI Gene ID	`LOC131022622`
GeneID	`131022622`
locus_tag	`-`
NCBI Name	`LOC131022622`
原始查询	`LOC131022622`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	replication factor C subunit 1
产物	replication factor C subunit 1
Arabidopsis	`AT5G22010` (RFC1) replication factor C1 [Source:NCBI gene (formerly Entrezgene);Acc:832261] identity=69.2; qcov=0.805; evalue=0; confidence=high
Rice	`Os11g0572100` (RFC1) - identity=60.5; qcov=1.04; evalue=0; confidence=high
GO	GO:0003677 (DNA binding); GO:0003689 (DNA clamp loader activity); GO:0005524 (ATP binding); GO:0005634 (nucleus); GO:0005663 (DNA replication factor C complex); GO:0006260 (DNA replication); GO:0006281 (DNA repair); GO:0016887 (ATP hydrolysis activity)
KEGG	smil03030 (DNA replication - Salvia miltiorrhiza (redroot sage)); smil03410 (Base excision repair - Salvia miltiorrhiza (redroot sage)); smil03420 (Nucleotide excision repair - Salvia miltiorrhiza (redroot sage)); smil03430 (Mismatch repair - Salvia miltiorrhiza (redroot sage))
Pfam	PF00004 (AAA); PF00533 (BRCT); PF03215 (Rad17); PF08519 (RFC1); PF12738 (PTCB-BRCT); PF25361 (AAA_lid_RFC1)
InterPro	-
eggNOG	-
NR	GeneID:131022622

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Nucleus	细胞核	1.00000	`XP_057808112.1`
2	Chromosome	染色体	0.40000	`XP_057808112.1`
3	Nucleoplasm	核质	0.40000	`XP_057808112.1`
4	Nucleolus	核仁	0.20000	`XP_057808112.1`
5	Centromere	着丝粒	0.00000	`XP_057808112.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr1:35519932`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C10
`Chr1:35519988`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B99
`Chr1:35520595`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	D5
`Chr1:35521891`	`CG`	`C`	1bp_deletion	frameshift_variant	38	A19,B108,B11,B115,B117,B132,B134,B136,B139,B16,B25,B42,B43,B46,B52,B60,B65,B70,B77,B8,B81,B83,B87,B89,B9,B98,C1,C12,C14,C20,C46,C5,C50,C53,C59,C65,C79,D7
`Chr1:35522517`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C6
`Chr1:35522828`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B95
`Chr1:35523333`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C83
`Chr1:35523343`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B103,C3
`Chr1:35523749`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B103,C3
`Chr1:35525594`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B21
`Chr1:35525636`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B78
`Chr1:35525964`	`G`	`A`	EMS_canonical_SNV	CDS_variant	3	A1,B47,C43

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057808112.1

991 aa

496

991

BRCT

PTCB-BRCT

Rad17

AAA

AAA_lid_RFC1

RFC1

PF00533 BRCT 245-320 aa PF12738 PTCB-BRCT 260-311 aa PF03215 Rad17 387-471 aa PF00004 AAA 442-554 aa PF25361 AAA_lid_RFC1 578-634 aa PF08519 RFC1 715-878 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057808112.1`	Pfam	`PF00533`	BRCT	Domain	245	320	2.6e-18	66.7	BRCA1 C Terminus (BRCT) domain
`XP_057808112.1`	Pfam	`PF12738`	PTCB-BRCT	Domain	260	311	3.3e-06	27.7	twin BRCT domain
`XP_057808112.1`	Pfam	`PF03215`	Rad17	Domain	387	471	2e-05	25.4	Rad17 P-loop domain
`XP_057808112.1`	Pfam	`PF00004`	AAA	Domain	442	554	2.4e-12	48.1	ATPase family associated with various cellular activities (AAA)
`XP_057808112.1`	Pfam	`PF25361`	AAA_lid_RFC1	Domain	578	634	2.8e-11	43.4	RFC1-like, AAA+ ATPase lid domain
`XP_057808112.1`	Pfam	`PF08519`	RFC1	Domain	715	878	1.4e-41	142.9	Replication factor RFC1 C terminal domain

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

1 records

下载 cDNA FASTA

XM_057952129.1
TTAGCCAACGCACAGTTTGATTTCGCGGCTCTCTTGAATCTCTTTGGGAGTTTCTTCCACTGCAAAACCCCTCCGCCTCTCTAACGAAAACGCACCAAATTTTGCGCAAAGATATTCCTGGTTCGAGTGCTCGCGCTTTGCGGACGTCGGAAGTAATCGTCAGATGCATTTCCGTAATCGAATGGTGCTTTGAAGTGGAGACTTGGCAGCGTGAAGCCAGTTTCTAGATTGAAAGATGTCTCAAGATATAAGAAAGTGGT...

CDS

1 records

下载 CDS FASTA

LOC131022622
ATGTCTCAAGATATAAGAAAGTGGTTCATGAAGCAACATGGTAAAGACGCAGGAAATGGCAATACATCGAACCAAGCAAAGCCATCTACACCTCAGAAGCCTCCTGTTGTACAAGGAGAGAAAGAATCTTCCAGTAGGAGGAAGACAAGCAAATATTTTTCTAAGGATGCACAGAGTGCAAAAGATGAGATGGAGGTTGAGGAAGTTTCTGCAAAGAAGGCACAAAAGGGTAGTACAGGGTCACCCAATGTGAAGCCTCC...

Protein

1 records

下载 Protein FASTA

XP_057808112.1
MSQDIRKWFMKQHGKDAGNGNTSNQAKPSTPQKPPVVQGEKESSSRRKTSKYFSKDAQSAKDEMEVEEVSAKKAQKGSTGSPNVKPPSGKKTRKFEISDDDDDDDDFATPTSRKGSVGASPNKKLKSGSGKGVARKIVDDIDEDDDQTVKSNAKSAGSGRGRGAKGSAVTQKTVESMDVDKSDAEDMDDKDVKSVKPGGRGRGGRGAPASGRGRGGGGRGGGFMNFGERKDPPHKGEKEVPEGAPECLNGLTFVISGTLD...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

下载 upstream 下载 downstream 下载 upstream + gene + downstream

JBrowse

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