Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr04G000809`

染色体: Chr04 坐标: 10124551-10128751 链方向: + 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr04G000809`
名称	SmilChr04G000809
描述	E3 ubiquitin-protein ligase APD2
染色体 / SeqID	Chr04 / NC_080390.1
坐标与链方向	10124551 - 10128751 / `+`

ID 对照

NCBI Gene ID	`LOC131022651`
GeneID	`131022651`
locus_tag	`-`
NCBI Name	`LOC131022651`
原始查询	`LOC131022651`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	E3 ubiquitin-protein ligase APD2
产物	E3 ubiquitin-protein ligase APD2
Arabidopsis	`AT5G01450` (APD2) RING/U-box superfamily protein [Source:NCBI gene (formerly Entrezgene);Acc:831860] identity=39; qcov=0.883; evalue=1.08e-94; confidence=medium
Rice	`Os07g0446100` (XBOS34) - identity=32.1; qcov=0.197; evalue=4.23e-08; confidence=weak
GO	-
KEGG	-
Pfam	PF13920 (zf-C3HC4_3); PF16040 (APD1-4_N); PF16041 (APD1-4_M)
InterPro	-
eggNOG	-
NR	GeneID:131022651

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Vacuole	液泡	0.80000	`XP_057808151.1`
2	Vacuole membrane	液泡膜	0.80000	`XP_057808151.1`
3	Endoplasmic reticulum	内质网	0.20000	`XP_057808151.1`
4	Golgi apparatus	高尔基体	0.20000	`XP_057808151.1`
5	Trans-golgi network	Trans-golgi network	0.20000	`XP_057808151.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr4:10124828`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	A3
`Chr4:10124886`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B75,D7,D8
`Chr4:10124891`	`G`	`A`	EMS_canonical_SNV	CDS_variant	3	B75,D7,D8
`Chr4:10124930`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	A3
`Chr4:10124952`	`TA`	`T`	1bp_deletion	frameshift_variant	2	B90,C87
`Chr4:10124952`	`TA`	`T`	1bp_deletion	CDS_variant	2	B90,C87
`Chr4:10124973`	`GT`	`G`	1bp_deletion	frameshift_variant	1	B62
`Chr4:10124973`	`GT`	`G`	1bp_deletion	CDS_variant	1	B62
`Chr4:10125441`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C50
`Chr4:10125642`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B44
`Chr4:10126928`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B24,B52
`Chr4:10127007`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C73

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057808151.1

427 aa

214

427

APD1-4_N

APD1-4_M

zf-C3HC4_3

PF16040 APD1-4_N 111-180 aa PF16041 APD1-4_M 205-311 aa PF13920 zf-C3HC4_3 374-420 aa

XP_057808152.1

427 aa

214

427

APD1-4_N

APD1-4_M

zf-C3HC4_3

PF16040 APD1-4_N 111-180 aa PF16041 APD1-4_M 205-311 aa PF13920 zf-C3HC4_3 374-420 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057808151.1`	Pfam	`PF16040`	APD1-4_N	Domain	111	180	7.7e-21	74.3	E3 ubiquitin-protein ligase APD1-4, N-terminal
`XP_057808151.1`	Pfam	`PF16041`	APD1-4_M	Domain	205	311	3.2e-27	95.6	E3 ubiquitin-protein ligase APD1-4, middle domain
`XP_057808151.1`	Pfam	`PF13920`	zf-C3HC4_3	Domain	374	420	4.7e-13	49.4	Zinc finger, C3HC4 type (RING finger)
`XP_057808152.1`	Pfam	`PF16040`	APD1-4_N	Domain	111	180	7.7e-21	74.3	E3 ubiquitin-protein ligase APD1-4, N-terminal
`XP_057808152.1`	Pfam	`PF16041`	APD1-4_M	Domain	205	311	3.2e-27	95.6	E3 ubiquitin-protein ligase APD1-4, middle domain
`XP_057808152.1`	Pfam	`PF13920`	zf-C3HC4_3	Domain	374	420	4.7e-13	49.4	Zinc finger, C3HC4 type (RING finger)

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

2 records

下载 cDNA FASTA

XM_057952169.1
GCGTCGTTTAACCACCATTCGCACGAAAACCCAACGACTTTTTGGTAAAAGAAAAAAATAAAAACAATAAAAAGAAATTCCAAAATCCAAAAACAGAGTCTCCATAAATCTCTCACGTACATCTTCAAAACCGAAGCAAAATCGCGATTTGATGGCGGAGGTGGAGCAGCCGGAGTCGCCGCCGCGGCCGTTTCCCACGAGGTCGCCGGAGTTTCCGCGTGATCAATTCCACGGAGATCGTTTCTCGCGCAATTACAACG...

XM_057952168.1
TTCCAAAATCCAAAAACAGAGTCTCCATAAATCTCTCACGTACATCTTCAAAACCGAAGCAAAATCGCGATTTGTAAGAGTTCTCCGCTCGCACGCGACCAAATTTTCAATTGTCGTCAAAAAAATCCCCACCCGTTTTCTTCAATTCGACAGGATGGCGGAGGTGGAGCAGCCGGAGTCGCCGCCGCGGCCGTTTCCCACGAGGTCGCCGGAGTTTCCGCGTGATCAATTCCACGGAGATCGTTTCTCGCGCAATTACA...

CDS

2 records

下载 CDS FASTA

LOC131022651
ATGGCGGAGGTGGAGCAGCCGGAGTCGCCGCCGCGGCCGTTTCCCACGAGGTCGCCGGAGTTTCCGCGTGATCAATTCCACGGAGATCGTTTCTCGCGCAATTACAACGGTGAATCGTCGTTGAACAATGGCGCCTCCACTGCGAATGCAGACGATCCCAAGTCGTGCTTTATAGCTTCGACCGCGTTCTCGTTTTTTGTAGCCATAACTATGGCATTTGGACTGTATTCTCCCGAGACTCTGCGTCTTGGACCTAATGC...

Protein

2 records

下载 Protein FASTA

XP_057808151.1
MAEVEQPESPPRPFPTRSPEFPRDQFHGDRFSRNYNGESSLNNGASTANADDPKSCFIASTAFSFFVAITMAFGLYSPETLRLGPNASILIQPNRLFVESIEVVEVGAAKGSMLFGFYKTPPLDATTTWQETHKISLPSSTHKEWVHYLNEGSQINISYSVSSWGLSSIGLVIAEGNAELAEWLKEPSYPDTTFSWNIIHGNGSIQKDILKSSMYYIGVGNLNPEIFTVHLNMSIKASLYNTAEPYYHCRPAEGKCTFPL...

XP_057808152.1
MAEVEQPESPPRPFPTRSPEFPRDQFHGDRFSRNYNGESSLNNGASTANADDPKSCFIASTAFSFFVAITMAFGLYSPETLRLGPNASILIQPNRLFVESIEVVEVGAAKGSMLFGFYKTPPLDATTTWQETHKISLPSSTHKEWVHYLNEGSQINISYSVSSWGLSSIGLVIAEGNAELAEWLKEPSYPDTTFSWNIIHGNGSIQKDILKSSMYYIGVGNLNPEIFTVHLNMSIKASLYNTAEPYYHCRPAEGKCTFPL...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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