Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr01G002004`

染色体: Chr01 坐标: 35540745-35544427 链方向: - 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr01G002004`
名称	SmilChr01G002004
描述	E3 ubiquitin-protein ligase RHF2A-like
染色体 / SeqID	Chr01 / NC_080387.1
坐标与链方向	35540745 - 35544427 / `-`

ID 对照

NCBI Gene ID	`LOC131022655`
GeneID	`131022655`
locus_tag	`-`
NCBI Name	`LOC131022655`
原始查询	`LOC131022655`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	E3 ubiquitin-protein ligase RHF2A-like
产物	E3 ubiquitin-protein ligase RHF2A-like
Arabidopsis	`AT5G22000` (RHF2A) RING-H2 group F2A [Source:NCBI gene (formerly Entrezgene);Acc:832260] identity=59.2; qcov=0.883; evalue=2.2e-126; confidence=high
Rice	`Os11g0572500` (OsRFP) - identity=56.5; qcov=0.963; evalue=1.68e-134; confidence=high
GO	GO:0004842 (ubiquitin-protein transferase activity)
KEGG	-
Pfam	PF00097 (zf-C3HC4); PF13639 (zf-RING_2); PF13923 (zf-C3HC4_2); PF17123 (zf-RING_11)
InterPro	-
eggNOG	-
NR	GeneID:131022655

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Cytoplasm	细胞质	1.00000	`XP_057808159.1`
2	Cytosol	细胞质基质	1.00000	`XP_057808159.1`
3	Nucleoplasm	核质	0.20000	`XP_057808164.1`
4	Nucleus	细胞核	0.00000	`XP_057808159.1`
5	Chromosome	染色体	0.00000	`XP_057808159.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr1:35541289`	`G`	`A`	EMS_canonical_SNV	CDS_variant	5	A5,B108,B35,B76,D5
`Chr1:35541494`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C51
`Chr1:35541640`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	A20,B18,B50
`Chr1:35541641`	`G`	`A`	EMS_canonical_SNV	stop_gained	18	A22,B122,B23,B27,B31,B44,B67,B84,B88,B89,B91,C11,C31,C49,C60,C69,C71,D8
`Chr1:35541720`	`C`	`T`	EMS_canonical_SNV	CDS_variant	4	A10,B12,B121,B122
`Chr1:35541876`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C20
`Chr1:35542081`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C43
`Chr1:35542114`	`G`	`A`	EMS_canonical_SNV	CDS_variant	5	B106,B62,B8,C25,C59
`Chr1:35542120`	`C`	`T`	EMS_canonical_SNV	CDS_variant	5	B106,B62,B8,C25,C59
`Chr1:35542123`	`G`	`A`	EMS_canonical_SNV	CDS_variant	5	B106,B62,B8,C25,C59
`Chr1:35542389`	`G`	`A`	EMS_canonical_SNV	CDS_variant	5	B106,B52,C39,C44,C81

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057808159.1

409 aa

204

409

zf-RING_2

zf-RING_11

zf-C3HC4

zf-C3HC4_2

PF13639 zf-RING_2 28-70 aa PF17123 zf-RING_11 30-57 aa PF00097 zf-C3HC4 30-70 aa PF13923 zf-C3HC4_2 30-70 aa

XP_057808164.1

377 aa

188

377

zf-RING_2

zf-RING_11

zf-C3HC4

zf-C3HC4_2

PF13639 zf-RING_2 28-70 aa PF17123 zf-RING_11 30-57 aa PF00097 zf-C3HC4 30-70 aa PF13923 zf-C3HC4_2 30-70 aa

XP_057808170.1

377 aa

188

377

zf-RING_2

zf-RING_11

zf-C3HC4

zf-C3HC4_2

PF13639 zf-RING_2 28-70 aa PF17123 zf-RING_11 30-57 aa PF00097 zf-C3HC4 30-70 aa PF13923 zf-C3HC4_2 30-70 aa

XP_057808175.1

377 aa

188

377

zf-RING_2

zf-RING_11

zf-C3HC4

zf-C3HC4_2

PF13639 zf-RING_2 28-70 aa PF17123 zf-RING_11 30-57 aa PF00097 zf-C3HC4 30-70 aa PF13923 zf-C3HC4_2 30-70 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057808159.1`	Pfam	`PF13639`	zf-RING_2	Domain	28	70	1e-09	39.2	Ring finger domain
`XP_057808159.1`	Pfam	`PF17123`	zf-RING_11	Domain	30	57	5.4e-07	29.9	RING-like zinc finger
`XP_057808159.1`	Pfam	`PF00097`	zf-C3HC4	Domain	30	70	5.5e-06	26.9	Zinc finger, C3HC4 type (RING finger)
`XP_057808159.1`	Pfam	`PF13923`	zf-C3HC4_2	Domain	30	70	6.7e-07	29.7	Zinc finger, C3HC4 type (RING finger)
`XP_057808164.1`	Pfam	`PF13639`	zf-RING_2	Domain	28	70	9.4e-10	39.3	Ring finger domain
`XP_057808164.1`	Pfam	`PF17123`	zf-RING_11	Domain	30	57	4.9e-07	30.1	RING-like zinc finger
`XP_057808164.1`	Pfam	`PF00097`	zf-C3HC4	Domain	30	70	5.5e-06	26.9	Zinc finger, C3HC4 type (RING finger)
`XP_057808164.1`	Pfam	`PF13923`	zf-C3HC4_2	Domain	30	70	6.2e-07	29.8	Zinc finger, C3HC4 type (RING finger)
`XP_057808170.1`	Pfam	`PF13639`	zf-RING_2	Domain	28	70	9.4e-10	39.3	Ring finger domain
`XP_057808170.1`	Pfam	`PF17123`	zf-RING_11	Domain	30	57	4.9e-07	30.1	RING-like zinc finger
`XP_057808170.1`	Pfam	`PF00097`	zf-C3HC4	Domain	30	70	5.5e-06	26.9	Zinc finger, C3HC4 type (RING finger)
`XP_057808170.1`	Pfam	`PF13923`	zf-C3HC4_2	Domain	30	70	6.2e-07	29.8	Zinc finger, C3HC4 type (RING finger)
`XP_057808175.1`	Pfam	`PF13639`	zf-RING_2	Domain	28	70	9.4e-10	39.3	Ring finger domain
`XP_057808175.1`	Pfam	`PF17123`	zf-RING_11	Domain	30	57	4.9e-07	30.1	RING-like zinc finger
`XP_057808175.1`	Pfam	`PF00097`	zf-C3HC4	Domain	30	70	5.5e-06	26.9	Zinc finger, C3HC4 type (RING finger)
`XP_057808175.1`	Pfam	`PF13923`	zf-C3HC4_2	Domain	30	70	6.2e-07	29.8	Zinc finger, C3HC4 type (RING finger)

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

4 records

下载 cDNA FASTA

XM_057952187.1
ACCAGGCATTACTCCTTTCCTCCTCCAAAGATGTGTCTGTGTGTCAGTGTGTGTTGTGTGTTTTTTTGTGGGCTCAGTCATGTCATCAGACTCACCACCGCCAAAAAAGTAAAAGAAAGAGAAAAAAAGAAAGAGAGAGAGAGAGAAAACAAGAAAAAGGCTAGAGAAGAAATAATTATTCTCCAAAAACCAACAAAAAGAAAAGTCCAAAGAAATAGTAATTATTTTTTCCTCATTCTCATTTCTTTCCCCAAATTCAC...

XM_057952181.1
TGTGTCAGTGTGTGTTGTGTGTTTTTTTGTGGGCTCAGTCATGTCATCAGACTCACCACCGCCAAAAAAGTAAAAGAAAGAGAAAAAAAGAAAGAGAGAGAGAGAGAAAACAAGAAAAAGGCTAGAGAAGAAATAATTATTCTCCAAAAACCAACAAAAAGAAAAGTCCAAAGAAATAGTAATTATTTTTTCCTCATTCTCATTTCTTTCCCCAAATTCACTCATTCAGGATGGAAGAGGAAAATGTGTCCGAGAAAAGT...

CDS

4 records

下载 CDS FASTA

LOC131022655
ATGGAAGAGGAAAATGTGTCCGAGAAAAGTTTGACCTCCGCAGCAGCTTTTGTGGAAGGAGGAATTCAAGATGCATGTGATGATTCGTGTAGTATATGCCTCGAAGCTTTTTGTGACAGTGATCCTTCCACGGTAACTAGCTGCAAGCACGAGTTTCATCTTCAGTGCATTCTTGAATGGTGCCAGAGGAGCTCACAGTGCCCAATGTGCTGGCAGCCCATCGGCTTGAAGGATCCCAGTAGCCAGGAGCTGCTCGAAGG...

Protein

4 records

下载 Protein FASTA

XP_057808159.1
MEEENVSEKSLTSAAAFVEGGIQDACDDSCSICLEAFCDSDPSTVTSCKHEFHLQCILEWCQRSSQCPMCWQPIGLKDPSSQELLEGVEHERNIRMNPPRNTTIFHHPTLGDFELQHLPVTASESELEERIIQHLAAAAAMGRARHFARREGQRNRTSAQGRPQFLVFSTHPNGASAASPAAQREGGFPPAVMIAGPNSPFITVEDSEQLIPRISSARADQHMGTGSASSASATAASRPSSNRSPSQTSSGSQDRAGPSD...

XP_057808164.1
MEEENVSEKSLTSAAAFVEGGIQDACDDSCSICLEAFCDSDPSTVTSCKHEFHLQCILEWCQRSSQCPMCWQPIGLKDPSSQELLEGVEHERNIRMNPPRNTTIFHHPTLGDFELQHLPVTASESELEERIIQHLAAAAAMGRARHFARREGQRNRTSAQGRPQFLVFSTHPNGASAASPAAQREGGFPPAVMIAGPNSPFITVEDSEQLIPRISSARADQHMGTGSASSASATAASRPSSNRSPSQTSSGSQDRAGPSD...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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