Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr05G003049`

染色体: Chr05 坐标: 38269333-38271495 链方向: + 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr05G003049`
名称	SmilChr05G003049
描述	probable serine/threonine-protein kinase PBL23
染色体 / SeqID	Chr05 / NC_080391.1
坐标与链方向	38269333 - 38271495 / `+`

ID 对照

NCBI Gene ID	`LOC131024335`
GeneID	`131024335`
locus_tag	`-`
NCBI Name	`LOC131024335`
原始查询	`LOC131024335`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	probable serine/threonine-protein kinase PBL23
产物	probable serine/threonine-protein kinase PBL23
Arabidopsis	`AT3G20530` (AT3G20530) Protein kinase superfamily protein [Source:NCBI gene (formerly Entrezgene);Acc:821599] identity=79.4; qcov=0.979; evalue=6.12e-139; confidence=high
Rice	`Os02g0513000` (OsRLCK72) - identity=72.7; qcov=1; evalue=3.25e-129; confidence=high
GO	GO:0004672 (protein kinase activity); GO:0005524 (ATP binding); GO:0006468 (protein phosphorylation)
KEGG	-
Pfam	PF00069 (Pkinase); PF07714 (PK_Tyr_Ser-Thr)
InterPro	-
eggNOG	-
NR	GeneID:131024335

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Cell membrane	细胞膜	1.00000	`XP_057809829.1`
2	Cytosol	细胞质基质	0.60000	`XP_057809830.1`
3	Nucleus	细胞核	0.00000	`XP_057809829.1`
4	Chromosome	染色体	0.00000	`XP_057809829.1`
5	Centromere	着丝粒	0.00000	`XP_057809829.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr5:38269657`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B69,B9,C5
`Chr5:38269680`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B40
`Chr5:38270094`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B121,C4
`Chr5:38270199`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B108,B122
`Chr5:38270310`	`CA`	`C`	1bp_deletion	frameshift_variant	1	B112
`Chr5:38270310`	`CA`	`C`	1bp_deletion	CDS_variant	1	B112
`Chr5:38270426`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B73
`Chr5:38270871`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	A10,B42
`Chr5:38270947`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	A4,C31,C36

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057809829.1

361 aa

180

361

Pkinase

PK_Tyr_Ser-Thr

PF00069 Pkinase 78-348 aa PF07714 PK_Tyr_Ser-Thr 78-349 aa

XP_057809830.1

238 aa

119

238

Pkinase

PK_Tyr_Ser-Thr

PF00069 Pkinase 2-225 aa PF07714 PK_Tyr_Ser-Thr 2-226 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057809829.1`	Pfam	`PF00069`	Pkinase	Domain	78	348	6e-45	154.4	Protein kinase domain
`XP_057809829.1`	Pfam	`PF07714`	PK_Tyr_Ser-Thr	Domain	78	349	3.6e-45	155.0	Protein tyrosine and serine/threonine kinase
`XP_057809830.1`	Pfam	`PF00069`	Pkinase	Domain	2	225	6.8e-37	128.0	Protein kinase domain
`XP_057809830.1`	Pfam	`PF07714`	PK_Tyr_Ser-Thr	Domain	2	226	5.6e-34	118.3	Protein tyrosine and serine/threonine kinase

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

2 records

下载 cDNA FASTA

XM_057953846.1
TAACCACACGCTAATTATAGGAAAATTGACGGAGTCAAAATATAGTGTATTTGAAAACAACTGGCTATGAGGGCAGAAGAAAAGTTAAACGACGCCATTGAATCTTCTTCAGCTTCTTGAGAGACCGGAAAGCGCAGCTGGTTTATCGAGAGCTGAAGAAATTCAATTCATTATGCGAATAGGAAAAGCAGATTGATCGCAAAATATAAAGGGGGGGATTGAAATGGTGGGGTTGAGGTGATCTCGAGGAAGGATGTTCT...

XM_057953847.1
GTGGGGTTGAGGTGATCTCGAGGAAGGATGTTCTGTTTTTCTTGTTGCATGCCACAAGACAACGTCAACCAGGAGCCCTCCACTAAACCATACCAAGACACAAAACCCCTTGCCTCTATTGCTAATCTATCCTTGAAAACTGATTCGCAGATAGCACCAGGCAAAGGTATGTAGCTGAAGAAATAGAAAAACTTGGAAAGAACAATATCTCTGCCGATATCTTCACTTTCCACGACCTGTCCGTCGCCACAAACAACTTC...

CDS

2 records

下载 CDS FASTA

LOC131024335
ATGTTCTGTTTTTCTTGTTGCATGCCACAAGACAACGTCAACCAGGAGCCCTCCACTAAACCATACCAAGACACAAAACCCCTTGCCTCTATTGCTAATCTATCCTTGAAAACTGATAGCACCAGGCAAAGGTATGTAGCTGAAGAAATAGAAAAACTTGGAAAGAACAATATCTCTGCCGATATCTTCACTTTCCACGACCTGTCCGTCGCCACAAACAACTTCGACGCCGATAATTTGGTAGGCGAAGGCGGCTTTGG...

LOC131024335
ATGATGCTCAGTCTTCTTCACCATCAGAACCTTGTCAATCTCGTGGGATACTGCTCCGAGGGCGACCAAAGAATCCTTGTTTACGAGTACATGATCAATGGCTCCTTGGAGGATCACCTGTTAAATACGAGTCCCGAGAAAAAGGGGCTTACGTGGGATGTGCGGATGAAAATCGCACATGGAGCAGCAAGAGGGCTAGAGTACTTGCACGAAACAGCGAGTCCTCCGGTGATCTACCGAGACTTCAAGTCCTCCAACAT...

Protein

2 records

下载 Protein FASTA

XP_057809829.1
MFCFSCCMPQDNVNQEPSTKPYQDTKPLASIANLSLKTDSTRQRYVAEEIEKLGKNNISADIFTFHDLSVATNNFDADNLVGEGGFGRVYKGRINIQGKDEDVAVKQLDRNGFQGNREFLVEVMMLSLLHHQNLVNLVGYCSEGDQRILVYEYMINGSLEDHLLNTSPEKKGLTWDVRMKIAHGAARGLEYLHETASPPVIYRDFKSSNILLDANFNPKLSDFGLAKIGPVGERPHVSTRVMGTYGYCAPEYASTGQLTT...

XP_057809830.1
MMLSLLHHQNLVNLVGYCSEGDQRILVYEYMINGSLEDHLLNTSPEKKGLTWDVRMKIAHGAARGLEYLHETASPPVIYRDFKSSNILLDANFNPKLSDFGLAKIGPVGERPHVSTRVMGTYGYCAPEYASTGQLTTKSDVYSFGVVFLEMITGRRVIDNARPSQEHNLIEWAQPLFKDKKKFHTMADPLLEGNYPEKSLYQALAIAAMCLQEDAASRPLISDVVTALEFLSPQPGDP

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

下载 upstream 下载 downstream 下载 upstream + gene + downstream

JBrowse

跳转到基因组浏览器