Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr05G000003`

染色体: Chr05 坐标: 3422-9018 链方向: - 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr05G000003`
名称	SmilChr05G000003
描述	thiamine biosynthetic bifunctional enzyme TH1, chloroplastic
染色体 / SeqID	Chr05 / NC_080391.1
坐标与链方向	3422 - 9018 / `-`

ID 对照

NCBI Gene ID	`LOC131025448`
GeneID	`131025448`
locus_tag	`-`
NCBI Name	`LOC131025448`
原始查询	`LOC131025448`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	thiamine biosynthetic bifunctional enzyme TH1, chloroplastic
产物	thiamine biosynthetic bifunctional enzyme TH1%2C chloroplastic
Arabidopsis	`AT1G22940` (TH1) thiamine biosynthesis protein [Source:NCBI gene (formerly Entrezgene);Acc:838901] identity=69.2; qcov=0.901; evalue=0; confidence=high
Rice	`Os12g0192500` - identity=69.5; qcov=0.896; evalue=0; confidence=high
GO	GO:0004789 (thiamine-phosphate diphosphorylase activity); GO:0008902 (hydroxymethylpyrimidine kinase activity); GO:0008972 (phosphomethylpyrimidine kinase activity); GO:0009228 (thiamine biosynthetic process); GO:0009507 (chloroplast)
KEGG	smil00730 (Thiamine metabolism - Salvia miltiorrhiza (redroot sage)); smil01100 (Metabolic pathways - Salvia miltiorrhiza (redroot sage)); smil01240 (Biosynthesis of cofactors - Salvia miltiorrhiza (redroot sage))
Pfam	PF00294 (PfkB); PF02581 (TMP-TENI); PF08543 (Phos_pyr_kin)
InterPro	-
eggNOG	-
NR	GeneID:131025448

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Chloroplast	叶绿体	1.00000	`XP_057811220.1`
2	Chloroplast stroma	叶绿体基质	0.60000	`XP_057811220.1`
3	Mitochondrion	线粒体	0.40000	`XP_057811220.1`
4	Nucleus	细胞核	0.00000	`XP_057811220.1`
5	Chromosome	染色体	0.00000	`XP_057811220.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr5:3715`	`TC`	`T`	1bp_deletion	frameshift_variant	1	B122
`Chr5:3715`	`TC`	`T`	1bp_deletion	CDS_variant	1	B122
`Chr5:3718`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B122
`Chr5:3903`	`C`	`T`	EMS_canonical_SNV	CDS_variant	4	A1,B90,B92,C84
`Chr5:3936`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B75,B9
`Chr5:4013`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B104
`Chr5:4062`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	C37,D5
`Chr5:4090`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	A2
`Chr5:4846`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	C2,C64,C66
`Chr5:8638`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B103
`Chr5:8671`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	A11,B50

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057811220.1

548 aa

274

548

Phos_pyr_kin

PfkB

TMP-TENI

PF08543 Phos_pyr_kin 66-310 aa PF00294 PfkB 157-290 aa PF02581 TMP-TENI 340-525 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057811220.1`	Pfam	`PF08543`	Phos_pyr_kin	Family	66	310	3.7e-88	295.8	Phosphomethylpyrimidine kinase
`XP_057811220.1`	Pfam	`PF00294`	PfkB	Domain	157	290	3.6e-07	30.5	pfkB family carbohydrate kinase
`XP_057811220.1`	Pfam	`PF02581`	TMP-TENI	Domain	340	525	5.9e-63	212.2	Thiamine monophosphate synthase

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

1 records

下载 cDNA FASTA

XM_057955237.1
TTTAAAATCTTTGCCACCATCAAAACACACATAGTGGAAAAAGCTTCACGCACTACACAGTATATGCTTTCCCGCAAACGTCCATCCAAACCCTTCGCCACCCCCAAATGATTTGGCCAAATCCACCAAAATATAATTGAAAGTCCAAATTTGAATCATTTGATACACGTGTCGCAGCATACGGCGTTCATCGATGATGATAGATATTCACCTGCACGCCAATTCCCAACTCCATCTTCTCAACTTCACTCATTCTCATC...

CDS

1 records

下载 CDS FASTA

LOC131025448
ATGATGATAGATATTCACCTGCACGCCAATTCCCAACTCCATCTTCTCAACTTCACTCATTCTCATCACCGTAATTTCCCGCATTTTGTCTCTAGAAGGTTCCGTACAACTGGGGTTCCTCATCTGCGAGCTATGCAGCAACAACAAGAGCCGGCCAATGTACGAGTCCCGCACGTGCTCACCGTCGCTGGCTCCGATTCCGGCGCCGGCGCTGGAATCCAAGCTGACTTGAAGGCTTGCGCTGCCCGCGGTGTTTACTG...

Protein

1 records

下载 Protein FASTA

XP_057811220.1
MMIDIHLHANSQLHLLNFTHSHHRNFPHFVSRRFRTTGVPHLRAMQQQQEPANVRVPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQNTAGVQGVNVIPEDFVGKQLRSVLSDMHPDVVKTGMLPSPGIVKVLCQSLKEFQVRALVVDPVMVSTSGDVLAGPSVLDTLREELLPLADIVTPNLREASALLGGVALETVADMHFAAKKIYDLGCRNVLVKGGDLPNCSDAVDVLFDGKQLFEFRAARIRTS...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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JBrowse

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