当前解析结果
SmilChr01G000222| 统一新 Gene ID | SmilChr01G000222 |
|---|---|
| 名称 | SmilChr01G000222 |
| 描述 | isoamylase 2, chloroplastic |
| 染色体 / SeqID | Chr01 / NC_080387.1 |
| 坐标与链方向 | 3924057 - 3927317 / - |
| NCBI Gene ID | LOC131007322 |
|---|---|
| GeneID | 131007322 |
| locus_tag | - |
| NCBI Name | LOC131007322 |
| 原始查询 | SmilChr01G000222 |
| 来源 | GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog |
|---|---|
| 描述 | isoamylase 2, chloroplastic |
| 产物 | isoamylase 2%2C chloroplastic |
| Arabidopsis | AT1G03310 (DBE1)debranching enzyme 1 [Source:NCBI gene (formerly Entrezgene);Acc:839531] identity=53.7; qcov=0.939; evalue=0; confidence=high |
| Rice | Os05g0393700 (OsISA2)- identity=50; qcov=0.836; evalue=0; confidence=high |
| GO | GO:0019156 (isoamylase activity); GO:0019252 (starch biosynthetic process) |
| KEGG | smil00500 (Starch and sucrose metabolism - Salvia miltiorrhiza (redroot sage)); smil01100 (Metabolic pathways - Salvia miltiorrhiza (redroot sage)); smil01110 (Biosynthesis of secondary metabolites - Salvia miltiorrhiza (redroot sage)) |
| Pfam | PF00128 (Alpha-amylase); PF02922 (CBM_48); PF21156 (ISOA1-3_C) |
| InterPro | - |
| eggNOG | - |
| NR | GeneID:131007322 |
| Rank | 定位位置 | 中文 | Score | Protein ID |
|---|---|---|---|---|
| 1 | Chloroplast | 叶绿体 | 1.00000 | XP_057790443.1 |
| 2 | Chloroplast stroma | 叶绿体基质 | 1.00000 | XP_057790443.1 |
| 3 | Nucleus | 细胞核 | 0.00000 | XP_057790443.1 |
| 4 | Chromosome | 染色体 | 0.00000 | XP_057790443.1 |
| 5 | Centromere | 着丝粒 | 0.00000 | XP_057790443.1 |
下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。
| 位置 | REF | ALT | 类型 | 影响 | 携带材料数 | 材料 |
|---|---|---|---|---|---|---|
Chr1:3924473 |
G |
A |
EMS_canonical_SNV | CDS_variant | 1 | D11 |
Chr1:3924475 |
AG |
A |
1bp_deletion | frameshift_variant | 1 | B71 |
Chr1:3924475 |
AG |
A |
1bp_deletion | CDS_variant | 1 | B71 |
Chr1:3924514 |
G |
A |
EMS_canonical_SNV | CDS_variant | 2 | B138,B55 |
Chr1:3924662 |
C |
T |
EMS_canonical_SNV | CDS_variant | 1 | D1 |
Chr1:3924680 |
C |
T |
EMS_canonical_SNV | CDS_variant | 1 | B71 |
Chr1:3924772 |
G |
A |
EMS_canonical_SNV | CDS_variant | 1 | B104 |
Chr1:3924829 |
C |
T |
EMS_canonical_SNV | CDS_variant | 1 | D1 |
Chr1:3924857 |
G |
A |
EMS_canonical_SNV | CDS_variant | 2 | B49,B50 |
Chr1:3924872 |
C |
T |
EMS_canonical_SNV | CDS_variant | 3 | B41,C10,C74 |
Chr1:3924873 |
G |
A |
EMS_canonical_SNV | CDS_variant | 1 | D1 |
Chr1:3924932 |
C |
T |
EMS_canonical_SNV | CDS_variant | 2 | B21,C35 |
结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。
XP_057790443.1PF02922
CBM_48 210-297 aa
PF00128
Alpha-amylase 360-446 aa
PF21156
ISOA1-3_C 735-805 aa
| Protein ID | 来源 | 结构域编号 | 结构域名称 | 类型 | 起始 aa | 终止 aa | E-value | Score | 说明 |
|---|---|---|---|---|---|---|---|---|---|
XP_057790443.1 |
Pfam | PF02922 |
CBM_48 | Domain | 210 | 297 | 3.5e-11 | 44.0 | Carbohydrate-binding module 48 (Isoamylase N-terminal domain) |
XP_057790443.1 |
Pfam | PF00128 |
Alpha-amylase | Domain | 360 | 446 | 1.2e-10 | 41.9 | Alpha amylase, catalytic domain |
XP_057790443.1 |
Pfam | PF21156 |
ISOA1-3_C | Domain | 735 | 805 | 4e-10 | 40.6 | Isoamylase 1-3, C-terminal |
每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。
1 records
下载 cDNA FASTA1 records
下载 CDS FASTA1 records
下载 Protein FASTAupstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。