当前解析结果
SmilChr01G001707| 统一新 Gene ID | SmilChr01G001707 |
|---|---|
| 名称 | SmilChr01G001707 |
| 描述 | protein argonaute MEL1 |
| 染色体 / SeqID | Chr01 / NC_080387.1 |
| 坐标与链方向 | 31496041 - 31502433 / - |
| NCBI Gene ID | LOC131020228 |
|---|---|
| GeneID | 131020228 |
| locus_tag | - |
| NCBI Name | LOC131020228 |
| 原始查询 | SmilChr01G001707 |
| 来源 | GFF basic annotation; NCBI GO GAF; Pfam-A/HMMER; Arabidopsis/Rice homolog |
|---|---|
| 描述 | protein argonaute MEL1 |
| 产物 | protein argonaute MEL1 |
| Arabidopsis | AT2G27880 (AGO5)Argonaute family protein [Source:NCBI gene (formerly Entrezgene);Acc:817334] identity=59.2; qcov=0.971; evalue=0; confidence=high |
| Rice | Os03g0800200 (MEL1)- identity=60.5; qcov=0.931; evalue=0; confidence=high |
| GO | GO:0003723 (RNA binding); GO:0005515 (protein binding); GO:0005737 (cytoplasm); GO:0035194 (regulatory ncRNA-mediated post-transcriptional gene silencing) |
| KEGG | - |
| Pfam | PF02170 (PAZ); PF02171 (Piwi); PF08699 (ArgoL1); PF16486 (ArgoN); PF16487 (ArgoMid); PF16488 (ArgoL2) |
| InterPro | - |
| eggNOG | - |
| NR | GeneID:131020228 |
| Rank | 定位位置 | 中文 | Score | Protein ID |
|---|---|---|---|---|
| 1 | Cytoplasm | 细胞质 | 1.00000 | XP_057804913.1 |
| 2 | Cytoplasmic granule | 细胞质颗粒 | 1.00000 | XP_057804913.1 |
| 3 | Nucleus | 细胞核 | 0.60000 | XP_057804913.1 |
| 4 | Cytoplasmic ribonucleoprotein granule | Cytoplasmic ribonucleoprotein granule | 0.60000 | XP_057804913.1 |
| 5 | Cytosol | 细胞质基质 | 0.60000 | XP_057804913.1 |
下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。
| 位置 | REF | ALT | 类型 | 影响 | 携带材料数 | 材料 |
|---|---|---|---|---|---|---|
Chr1:31496342 |
C |
T |
EMS_canonical_SNV | CDS_variant | 3 | B107,B54,B63 |
Chr1:31496408 |
G |
A |
EMS_canonical_SNV | CDS_variant | 1 | A23 |
Chr1:31496723 |
TA |
T |
1bp_deletion | frameshift_variant | 1 | A4 |
Chr1:31496723 |
TA |
T |
1bp_deletion | CDS_variant | 1 | A4 |
Chr1:31497637 |
GGA |
G |
2bp_deletion | frameshift_variant | 16 | A10,A18,A21,B12,B2,B28,B32,B79,B82,C18,C37,C73,C86,C87,D4,D5 |
Chr1:31497702 |
G |
A |
EMS_canonical_SNV | CDS_variant | 1 | C76 |
Chr1:31498003 |
G |
A |
EMS_canonical_SNV | stop_gained | 13 | A21,B12,B2,B28,B32,B79,C18,C37,C73,C86,C87,D4,D5 |
Chr1:31498494 |
G |
A |
EMS_canonical_SNV | CDS_variant | 1 | B55 |
Chr1:31498696 |
C |
T |
EMS_canonical_SNV | CDS_variant | 2 | B60,C17 |
Chr1:31499014 |
TTC |
T |
2bp_deletion | frameshift_variant | 14 | B111,B117,B137,B20,B21,B31,B40,B45,C4,C5,C51,C54,C57,C72 |
Chr1:31499168 |
C |
T |
EMS_canonical_SNV | CDS_variant | 5 | A15,A3,B114,B117,C5 |
Chr1:31499173 |
CG |
C |
1bp_deletion | frameshift_variant | 4 | A15,A3,B114,C5 |
结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。
XP_057804913.1PF16486
ArgoN 136-268 aa
PF08699
ArgoL1 279-328 aa
PF02170
PAZ 334-458 aa
PF16488
ArgoL2 469-515 aa
PF16487
ArgoMid 525-600 aa
PF02171
Piwi 621-942 aa
| Protein ID | 来源 | 结构域编号 | 结构域名称 | 类型 | 起始 aa | 终止 aa | E-value | Score | 说明 |
|---|---|---|---|---|---|---|---|---|---|
XP_057804913.1 |
Pfam | PF16486 |
ArgoN | Domain | 136 | 268 | 4.1e-33 | 115.5 | N-terminal domain of argonaute |
XP_057804913.1 |
Pfam | PF08699 |
ArgoL1 | Domain | 279 | 328 | 1.5e-17 | 63.7 | Argonaute linker 1 domain |
XP_057804913.1 |
Pfam | PF02170 |
PAZ | Domain | 334 | 458 | 3.4e-29 | 102.0 | PAZ domain |
XP_057804913.1 |
Pfam | PF16488 |
ArgoL2 | Family | 469 | 515 | 6.3e-15 | 55.8 | Argonaute linker 2 domain |
XP_057804913.1 |
Pfam | PF16487 |
ArgoMid | Domain | 525 | 600 | 1.9e-08 | 35.1 | Mid domain of argonaute |
XP_057804913.1 |
Pfam | PF02171 |
Piwi | Domain | 621 | 942 | 1.6e-110 | 369.8 | Piwi domain |
每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。
1 records
下载 cDNA FASTA1 records
下载 CDS FASTA1 records
下载 Protein FASTAupstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。