Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr01G005383`

染色体: Chr01 坐标: 71070234-71076852 链方向: - 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr01G005383`
名称	SmilChr01G005383
描述	3beta,22alpha-dihydroxysteroid 3-dehydrogenase
染色体 / SeqID	Chr01 / NC_080387.1
坐标与链方向	71070234 - 71076852 / `-`

ID 对照

NCBI Gene ID	`LOC131005712`
GeneID	`131005712`
locus_tag	`-`
NCBI Name	`LOC131005712`
原始查询	`SmilChr01G005383`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	3beta,22alpha-dihydroxysteroid 3-dehydrogenase
产物	3beta%2C22alpha-dihydroxysteroid 3-dehydrogenase
Arabidopsis	`AT5G05690` (CPD) Cytochrome P450 superfamily protein [Source:NCBI gene (formerly Entrezgene);Acc:830453] identity=77.7; qcov=0.921; evalue=0; confidence=high
Rice	`Os12g0139300` (OsCPD2) - identity=60.2; qcov=0.973; evalue=0; confidence=high
GO	GO:0004497 (monooxygenase activity); GO:0005506 (iron ion binding); GO:0007275 (multicellular organism development); GO:0010268 (brassinosteroid homeostasis); GO:0016125 (sterol metabolic process); GO:0016132 (brassinosteroid biosynthetic process); GO:0016705 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen); GO:0020037 (heme binding)
KEGG	smil00905 (Brassinosteroid biosynthesis - Salvia miltiorrhiza (redroot sage)); smil01100 (Metabolic pathways - Salvia miltiorrhiza (redroot sage)); smil01110 (Biosynthesis of secondary metabolites - Salvia miltiorrhiza (redroot sage))
Pfam	PF00067 (p450)
InterPro	-
eggNOG	-
NR	GeneID:131005712

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Endoplasmic reticulum	内质网	1.00000	`XP_057788736.1`
2	Endoplasmic reticulum membrane	内质网膜	1.00000	`XP_057788736.1`
3	Nucleus	细胞核	0.00000	`XP_057788736.1`
4	Chromosome	染色体	0.00000	`XP_057788736.1`
5	Centromere	着丝粒	0.00000	`XP_057788736.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr1:71070492`	`G`	`A`	EMS_canonical_SNV	CDS_variant	4	B122,B140,B83,C59
`Chr1:71070667`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B104
`Chr1:71070691`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B130
`Chr1:71074902`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B98,C52,C8
`Chr1:71074926`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	A23
`Chr1:71074935`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B138
`Chr1:71074956`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	C40,C82
`Chr1:71075055`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B130
`Chr1:71076122`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B103
`Chr1:71076270`	`G`	`A`	EMS_canonical_SNV	CDS_variant	3	B87,C14,C2
`Chr1:71076333`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	D10
`Chr1:71076344`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C45

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057788736.1

479 aa

240

479

p450

PF00067 p450 203-451 aa

XP_057788737.1

478 aa

239

478

p450

PF00067 p450 203-450 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057788736.1`	Pfam	`PF00067`	p450	Domain	203	451	1.2e-62	213.0	Cytochrome P450
`XP_057788737.1`	Pfam	`PF00067`	p450	Domain	203	450	4.5e-64	217.6	Cytochrome P450

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

2 records

下载 cDNA FASTA

XM_057932754.1
TAGGATTTTACTATATTTACCGAATAATCATATTCGGATTTACTGGTATTGGGCCTAGAAATCACGTTGAGTAATTTCTTCGTAAACCAACATAAATTTTACTTGATATGAACAAGCAAGGGGTAAAATTCACGATTCAACATATGTTAGGCCGACAAAGAAAAATAAAATGATATTGGATATAATGATAATTGGTTGGTTTTAGAAGCATGAGATTTTGGGCATTGCTGGCTTAGATTTGAAATAATAAATAGTATAAT...

XM_057932753.1
TAGGATTTTACTATATTTACCGAATAATCATATTCGGATTTACTGGTATTGGGCCTAGAAATCACGTTGAGTAATTTCTTCGTAAACCAACATAAATTTTACTTGATATGAACAAGCAAGGGGTAAAATTCACGATTCAACATATGTTAGGCCGACAAAGAAAAATAAAATGATATTGGATATAATGATAATTGGTTGGTTTTAGAAGCATGAGATTTTGGGCATTGCTGGCTTAGATTTGAAATAATAAATAGTATAAT...

CDS

2 records

下载 CDS FASTA

LOC131005712
ATGTTCGTTTTCACTCTCATCCTCTCCCTCCTCTCCCTAACCATCTTCGTCCTCCTCCGCAGCCGCGCGCGGCGGCGGGGGCGGCTGCCGCCGGGGAATCTGGGGCTCCCCTTCATCGGGGAGACGCTCCAGCTGATCTCCGCCTACAAGACGGAGAATCCGGAGCCGTTCATCGACGACCGGGTGGCGCGATACGGGTCGGTGTTCACGACCCACCTGTTCGGCGAGCCGACCGTGTTCTCGGCCGACCCGGAGGCGAA...

Protein

2 records

下载 Protein FASTA

XP_057788736.1
MFVFTLILSLLSLTIFVLLRSRARRRGRLPPGNLGLPFIGETLQLISAYKTENPEPFIDDRVARYGSVFTTHLFGEPTVFSADPEANRLILQNEGRLFESSYPGSISNLLGRHSLLLMRGSLHKRMHSLTMSFANSAIIRDHLLLDIDRLVRLNMDAWTARVLLMEEAKKITFQLTVKQLMSFDPCEWTENLMKEYMLVIEGFFTIPLPIFSTTYRRAIQARGKVAEALSLVVRERRRETERGETSKNDMLAALLDEQGG...

XP_057788737.1
MFVFTLILSLLSLTIFVLLRSRARRRGRLPPGNLGLPFIGETLQLISAYKTENPEPFIDDRVARYGSVFTTHLFGEPTVFSADPEANRLILQNEGRLFESSYPGSISNLLGRHSLLLMRGSLHKRMHSLTMSFANSAIIRDHLLLDIDRLVRLNMDAWTARVLLMEEAKKITFQLTVKQLMSFDPCEWTENLMKEYMLVIEGFFTIPLPIFSTTYRRAIQARGKVAEALSLVVRERRRETERGETSKNDMLAALLDEQGG...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

下载 upstream 下载 downstream 下载 upstream + gene + downstream

JBrowse

跳转到基因组浏览器