当前解析结果
SmilChr02G000928| 统一新 Gene ID | SmilChr02G000928 |
|---|---|
| 名称 | SmilChr02G000928 |
| 描述 | malate synthase, glyoxysomal-like |
| 染色体 / SeqID | Chr02 / NC_080388.1 |
| 坐标与链方向 | 16145166 - 16147101 / + |
| NCBI Gene ID | LOC131011365 |
|---|---|
| GeneID | 131011365 |
| locus_tag | - |
| NCBI Name | LOC131011365 |
| 原始查询 | SmilChr02G000928 |
| 来源 | GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog |
|---|---|
| 描述 | malate synthase, glyoxysomal-like |
| 产物 | malate synthase%2C glyoxysomal-like |
| Arabidopsis | AT5G03860 (MLS)malate synthase [Source:NCBI gene (formerly Entrezgene);Acc:831690] identity=77.3; qcov=0.971; evalue=0; confidence=high |
| Rice | Os04g0486950 (MS)- identity=71.9; qcov=0.982; evalue=0; confidence=high |
| GO | GO:0004474 (malate synthase activity); GO:0005737 (cytoplasm); GO:0006097 (glyoxylate cycle) |
| KEGG | smil00620 (Pyruvate metabolism - Salvia miltiorrhiza (redroot sage)); smil00630 (Glyoxylate and dicarboxylate metabolism - Salvia miltiorrhiza (redroot sage)); smil01100 (Metabolic pathways - Salvia miltiorrhiza (redroot sage)); smil01110 (Biosynthesis of secondary metabolites - Salvia miltiorrhiza (redroot sage)); smil01200 (Carbon metabolism - Salvia miltiorrhiza (redroot sage)) |
| Pfam | PF01274 (MS_TIM-barrel); PF20656 (MS_N); PF20659 (MS_C) |
| InterPro | - |
| eggNOG | - |
| NR | GeneID:131011365 |
| Rank | 定位位置 | 中文 | Score | Protein ID |
|---|---|---|---|---|
| 1 | Cytosol | 细胞质基质 | 1.00000 | XP_057795149.1 |
| 2 | Cytoplasm | 细胞质 | 0.60000 | XP_057795149.1 |
| 3 | Nucleus | 细胞核 | 0.00000 | XP_057795149.1 |
| 4 | Chromosome | 染色体 | 0.00000 | XP_057795149.1 |
| 5 | Centromere | 着丝粒 | 0.00000 | XP_057795149.1 |
下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。
| 位置 | REF | ALT | 类型 | 影响 | 携带材料数 | 材料 |
|---|---|---|---|---|---|---|
Chr2:16145436 |
G |
A |
EMS_canonical_SNV | CDS_variant | 3 | B28,B40,C60 |
Chr2:16145445 |
C |
T |
EMS_canonical_SNV | CDS_variant | 1 | C37 |
Chr2:16145886 |
C |
CAA |
2bp_insertion | frameshift_variant | 3 | A21,B76,C45 |
Chr2:16145886 |
C |
CAA |
2bp_insertion | CDS_variant | 3 | A21,B76,C45 |
Chr2:16146083 |
G |
A |
EMS_canonical_SNV | CDS_variant | 1 | C74 |
Chr2:16146488 |
G |
A |
EMS_canonical_SNV | CDS_variant | 1 | D4 |
Chr2:16146520 |
G |
A |
EMS_canonical_SNV | CDS_variant | 1 | C18 |
Chr2:16146569 |
C |
T |
EMS_canonical_SNV | CDS_variant | 1 | D10 |
Chr2:16146676 |
G |
A |
EMS_canonical_SNV | CDS_variant | 4 | B131,B42,B43,C50 |
Chr2:16146692 |
G |
A |
EMS_canonical_SNV | CDS_variant | 2 | B11,B135 |
Chr2:16146800 |
G |
A |
EMS_canonical_SNV | CDS_variant | 4 | B1,B102,C47,C8 |
Chr2:16146872 |
G |
A |
EMS_canonical_SNV | CDS_variant | 1 | D11 |
结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。
XP_057795149.1PF20656
MS_N 14-75 aa
PF01274
MS_TIM-barrel 167-416 aa
PF20659
MS_C 423-545 aa
| Protein ID | 来源 | 结构域编号 | 结构域名称 | 类型 | 起始 aa | 终止 aa | E-value | Score | 说明 |
|---|---|---|---|---|---|---|---|---|---|
XP_057795149.1 |
Pfam | PF20656 |
MS_N | Domain | 14 | 75 | 6.7e-18 | 64.7 | Malate synthase, N-terminal domain |
XP_057795149.1 |
Pfam | PF01274 |
MS_TIM-barrel | Domain | 167 | 416 | 3.3e-113 | 377.5 | Malate synthase, TIM barrel domain |
XP_057795149.1 |
Pfam | PF20659 |
MS_C | Domain | 423 | 545 | 2e-41 | 141.8 | Malate synthase, C-terminal |
每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。
1 records
下载 cDNA FASTA1 records
下载 CDS FASTA1 records
下载 Protein FASTAupstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。