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中文 EN

Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。
示例

当前解析结果

SmilChr02G002287

染色体: Chr02 坐标: 40408486-40415281 链方向: + 类型: protein_coding

基本信息

统一新 Gene IDSmilChr02G002287
名称SmilChr02G002287
描述DNA-directed RNA polymerase II subunit RPB2
染色体 / SeqIDChr02 / NC_080388.1
坐标与链方向40408486 - 40415281 / +

ID 对照

NCBI Gene IDLOC131013392
GeneID131013392
locus_tag-
NCBI NameLOC131013392
原始查询SmilChr02G002287

查看完整 ID 对照表

功能注释

来源GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述DNA-directed RNA polymerase II subunit RPB2
产物DNA-directed RNA polymerase II subunit RPB2
ArabidopsisAT4G21710 (NRPB2)
DNA-directed RNA polymerase family protein [Source:NCBI gene (formerly Entrezgene);Acc:828259]
identity=94; qcov=0.994; evalue=0; confidence=high
RiceOs03g0403100
-
identity=37.7; qcov=0.99; evalue=0; confidence=medium
GOGO:0003677 (DNA binding); GO:0003899 (DNA-directed RNA polymerase activity); GO:0005665 (RNA polymerase II, core complex); GO:0006351 (DNA-templated transcription); GO:0032549 (ribonucleoside binding)
KEGGsmil03020 (RNA polymerase - Salvia miltiorrhiza (redroot sage)); smil03420 (Nucleotide excision repair - Salvia miltiorrhiza (redroot sage))
PfamPF00562 (RNA_pol_Rpb2_6); PF04560 (RNA_pol_Rpb2_7); PF04561 (RNA_pol_Rpb2_2); PF04563 (RNA_pol_Rpb2_1); PF04565 (RNA_pol_Rpb2_3); PF04566 (RNA_pol_Rpb2_4); PF04567 (RNA_pol_Rpb2_5)
InterPro-
eggNOG-
NRGeneID:131013392

打开注释模块

亚细胞定位 Top 5

Rank定位位置中文ScoreProtein ID
1 Nucleus 细胞核 1.00000 XP_057797441.1
2 Nucleoplasm 核质 0.80000 XP_057797441.1
3 Nucleolus 核仁 0.80000 XP_057797442.1
4 Nucleolar fibrillar center Nucleolar fibrillar center 0.60000 XP_057797441.1
5 Chromosome 染色体 0.40000 XP_057797441.1

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置 REF ALT 类型 影响 携带材料数 材料
Chr2:40408686 C T EMS_canonical_SNV stop_gained 55 A13,A14,A18,A19,A24,A4,A6,A8,B100,B106,B11,B118,B12,B121,B122,B126,B130,B138,B16,B2,B31,B34,B41,B42,B43,B46,B47,B5,B50,B55,B60,B61,B66,B7,B72,B75,B86,C1,C10,C17,C26,C34,C35,C36,C37,C38,C40,C42,C51,C6,C7,C70,C74,C85,C86
Chr2:40408774 AT A 1bp_deletion frameshift_variant 4 B11,B31,B72,C35
Chr2:40408774 AT A 1bp_deletion CDS_variant 4 B11,B31,B72,C35
Chr2:40408785 G A EMS_canonical_SNV CDS_variant 1 B61
Chr2:40408796 G A EMS_canonical_SNV stop_gained 23 A4,B11,B12,B121,B122,B130,B2,B31,B34,B41,B42,B50,B60,B61,B66,B72,C35,C37,C40,C47,C6,C85,C87
Chr2:40408800 G A EMS_canonical_SNV CDS_variant 2 B122,B2
Chr2:40408807 G A EMS_canonical_SNV CDS_variant 1 A4
Chr2:40408826 G A EMS_canonical_SNV CDS_variant 1 B2
Chr2:40408838 G A EMS_canonical_SNV CDS_variant 1 B41
Chr2:40408847 C T EMS_canonical_SNV CDS_variant 2 B61,C40
Chr2:40408903 C T EMS_canonical_SNV CDS_variant 1 B2
Chr2:40408940 C T EMS_canonical_SNV CDS_variant 2 B122,B2

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057797441.1
1210 aa
N
1
605
1210
RNA_pol_Rpb2_1
RNA_pol_Rpb2_2
RNA_pol_Rpb2_3
RNA_pol_Rpb2_4
RNA_pol_Rpb2_5
RNA_pol_Rpb2_6
RNA_pol_Rpb2_7
C
PF04563 RNA_pol_Rpb2_1 56-465 aa PF04561 RNA_pol_Rpb2_2 226-417 aa PF04565 RNA_pol_Rpb2_3 490-554 aa PF04566 RNA_pol_Rpb2_4 589-650 aa PF04567 RNA_pol_Rpb2_5 675-726 aa PF00562 RNA_pol_Rpb2_6 733-1103 aa PF04560 RNA_pol_Rpb2_7 1105-1196 aa
XP_057797442.1
1094 aa
N
1
547
1094
RNA_pol_Rpb2_1
RNA_pol_Rpb2_2
RNA_pol_Rpb2_3
RNA_pol_Rpb2_4
RNA_pol_Rpb2_5
RNA_pol_Rpb2_6
RNA_pol_Rpb2_7
C
PF04563 RNA_pol_Rpb2_1 1-349 aa PF04561 RNA_pol_Rpb2_2 110-301 aa PF04565 RNA_pol_Rpb2_3 374-438 aa PF04566 RNA_pol_Rpb2_4 473-534 aa PF04567 RNA_pol_Rpb2_5 559-610 aa PF00562 RNA_pol_Rpb2_6 617-987 aa PF04560 RNA_pol_Rpb2_7 989-1080 aa
Protein ID 来源 结构域编号 结构域名称 类型 起始 aa 终止 aa E-value Score 说明
XP_057797441.1 Pfam PF04563 RNA_pol_Rpb2_1 Family 56 465 2.7e-79 266.1 RNA polymerase beta subunit
XP_057797441.1 Pfam PF04561 RNA_pol_Rpb2_2 Domain 226 417 2.5e-55 187.8 RNA polymerase Rpb2, domain 2
XP_057797441.1 Pfam PF04565 RNA_pol_Rpb2_3 Domain 490 554 4.7e-25 88.2 RNA polymerase Rpb2, domain 3
XP_057797441.1 Pfam PF04566 RNA_pol_Rpb2_4 Domain 589 650 1.2e-23 83.7 RNA polymerase Rpb2, domain 4
XP_057797441.1 Pfam PF04567 RNA_pol_Rpb2_5 Domain 675 726 5.4e-18 66.3 RNA polymerase Rpb2, domain 5
XP_057797441.1 Pfam PF00562 RNA_pol_Rpb2_6 Domain 733 1103 1.1e-142 476.3 RNA polymerase Rpb2, domain 6
XP_057797441.1 Pfam PF04560 RNA_pol_Rpb2_7 Domain 1105 1196 1e-35 122.7 RNA polymerase Rpb2, domain 7
XP_057797442.1 Pfam PF04563 RNA_pol_Rpb2_1 Family 1 349 6.5e-58 196.3 RNA polymerase beta subunit
XP_057797442.1 Pfam PF04561 RNA_pol_Rpb2_2 Domain 110 301 2.3e-55 187.9 RNA polymerase Rpb2, domain 2
XP_057797442.1 Pfam PF04565 RNA_pol_Rpb2_3 Domain 374 438 4e-25 88.5 RNA polymerase Rpb2, domain 3
XP_057797442.1 Pfam PF04566 RNA_pol_Rpb2_4 Domain 473 534 1e-23 83.9 RNA polymerase Rpb2, domain 4
XP_057797442.1 Pfam PF04567 RNA_pol_Rpb2_5 Domain 559 610 4.9e-18 66.5 RNA polymerase Rpb2, domain 5
XP_057797442.1 Pfam PF00562 RNA_pol_Rpb2_6 Domain 617 987 9e-143 476.6 RNA polymerase Rpb2, domain 6
XP_057797442.1 Pfam PF04560 RNA_pol_Rpb2_7 Domain 989 1080 8e-36 123.1 RNA polymerase Rpb2, domain 7

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

2 records

下载 cDNA FASTA
XM_057941458.1
AAAAAACAGTTCACCGCCCTATGAGTTTCGACCTATAAAAGGGCCGCCTCTCACAAATCTCGTTTATTTGCTTCCGCTCCAGTTCTCCCCCAAATCGGAGGGGTTTTCAGCTTGACTTCAATTGGACTTCAAAATCCGAAAATTCGAAGATTAATCGGTGAAAAACACTCGGAAAAATGTATATGGATGATGAGTACGAGCAGGGCGACAACGGCGAGGAGCAAGGGTACAACATTGGCGAGGAGTACGAAGACGGCGAG...
XM_057941459.1
GAAAATGGTTGTTTTAGTGAATGGGAGTGGTTCCGTGGTTAGGGTTTATCTGGGTTTTGTTTAGTGGTTTATGGTAATGCGGTTTGCAAAATGTAACTGCTGAGGTGAAGCTAGATGGAAGCAAGAGGTCTAAGGCAGAATTTCTAAGTTATTTGTGTTGGTTGTTTGATTGCGAGAACTCTATGGCGATTCGGGTGCCTTTAGCTGAAACTGCTAGACCTGAATTAAGAAGACTATATACAAGATCAGTTTCGGTCAGA...

CDS

2 records

下载 CDS FASTA
LOC131013392
ATGTATATGGATGATGAGTACGAGCAGGGCGACAACGGCGAGGAGCAAGGGTACAACATTGGCGAGGAGTACGAAGACGGCGAGGGCGACGGCGACGGCGATGGTGAGGAGGATATCACGCAGGAGGACGCCTGGGCGGTGATCAGCGCTTACTTTGAGGAGAAGGGTCTGGTGCGGCAGCAGCTCGACTCCTTCGACGAGTTCATCCAGAACACGATGCAGGAGATTGTCGACGAGTCGGCCGACATCGAGATCCGCCC...
LOC131013392
ATGATGACAGAATCTGATGGAGAAACAGCTACTTTGTTTCCCAAGGCTGCAAGGCTAAGGAATTTGACATATTCAGCTCCATTGTATGTTGATGTTACCAAGAAAGTCATAAAGAAAGGTCATGATGGCGAGGAAGTTACCGAGACTCAGGATTTCACAAAAGTCTTTATTGGAAAGGTTCCTATAATGCTGAGGTCAAGTTATTGCACACTATATCAGAATTCTGAGAAAGATTTGACAGAGCTTGGAGAGTGCCCATA...

Protein

2 records

下载 Protein FASTA
XP_057797441.1
MYMDDEYEQGDNGEEQGYNIGEEYEDGEGDGDGDGEEDITQEDAWAVISAYFEEKGLVRQQLDSFDEFIQNTMQEIVDESADIEIRPESQHNPGHQPDFAETIYKISFGQIYLSKPMMTESDGETATLFPKAARLRNLTYSAPLYVDVTKKVIKKGHDGEEVTETQDFTKVFIGKVPIMLRSSYCTLYQNSEKDLTELGECPYDQGGYFIINGSEKVLIAQEKMSTNHVYVFKKRQPNKYAYVAEVRSMAETQNRPPSTM...
XP_057797442.1
MMTESDGETATLFPKAARLRNLTYSAPLYVDVTKKVIKKGHDGEEVTETQDFTKVFIGKVPIMLRSSYCTLYQNSEKDLTELGECPYDQGGYFIINGSEKVLIAQEKMSTNHVYVFKKRQPNKYAYVAEVRSMAETQNRPPSTMFVRMLSRTSAKGGSSGQYIRATLPYIRTEIPIIIVFRALGFVADKDILEHICYNFQDTQMMELLRPSLEEAFVIQNQQVALDYIGKRGATVGVTKEKRIKYAKEILQKEMLPHVGV...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

下载 upstream 下载 downstream 下载 upstream + gene + downstream

JBrowse

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