Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr02G003133`

染色体: Chr02 坐标: 52353547-52356675 链方向: + 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr02G003133`
名称	SmilChr02G003133
描述	dihydrolipoyllysine-residue acetyltransferase component 5 of pyruvate dehydrogenase complex, chloroplastic-like
染色体 / SeqID	Chr02 / NC_080388.1
坐标与链方向	52353547 - 52356675 / `+`

ID 对照

NCBI Gene ID	`LOC131010136`
GeneID	`131010136`
locus_tag	`-`
NCBI Name	`LOC131010136`
原始查询	`SmilChr02G003133`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	dihydrolipoyllysine-residue acetyltransferase component 5 of pyruvate dehydrogenase complex, chloroplastic-like
产物	dihydrolipoyllysine-residue acetyltransferase component 5 of pyruvate dehydrogenase complex%2C chloroplastic-like
Arabidopsis	`AT1G34430` (EMB3003) 2-oxoacid dehydrogenases acyltransferase family protein [Source:NCBI gene (formerly Entrezgene);Acc:840346] identity=72.7; qcov=1.01; evalue=0; confidence=high
Rice	`Os12g0182200` - identity=72; qcov=0.974; evalue=0; confidence=high
GO	GO:0004742 (dihydrolipoyllysine-residue acetyltransferase activity); GO:0006086 (pyruvate decarboxylation to acetyl-CoA); GO:0009534 (chloroplast thylakoid); GO:0009941 (chloroplast envelope); GO:0045254 (pyruvate dehydrogenase complex)
KEGG	smil00010 (Glycolysis / Gluconeogenesis - Salvia miltiorrhiza (redroot sage)); smil00020 (Citrate cycle (TCA cycle) - Salvia miltiorrhiza (redroot sage)); smil00620 (Pyruvate metabolism - Salvia miltiorrhiza (redroot sage)); smil00785 (Lipoic acid metabolism - Salvia miltiorrhiza (redroot sage)); smil01100 (Metabolic pathways - Salvia miltiorrhiza (redroot sage)); smil01110 (Biosynthesis of secondary metabolites - Salvia miltiorrhiza (redroot sage)); smil01200 (Carbon metabolism - Salvia miltiorrhiza (redroot sage)); smil01210 (2-Oxocarboxylic acid metabolism - Salvia miltiorrhiza (redroot sage))
Pfam	PF00198 (2-oxoacid_dh); PF00364 (Biotin_lipoyl); PF02817 (E3_binding)
InterPro	-
eggNOG	-
NR	GeneID:131010136

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Chloroplast	叶绿体	1.00000	`XP_057793530.1`
2	Chloroplast stroma	叶绿体基质	0.60000	`XP_057793530.1`
3	Mitochondrion	线粒体	0.20000	`XP_057793530.1`
4	Nucleus	细胞核	0.00000	`XP_057793530.1`
5	Chromosome	染色体	0.00000	`XP_057793530.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr2:52353868`	`G`	`A`	EMS_canonical_SNV	CDS_variant	3	A21,B114,B4
`Chr2:52354043`	`G`	`A`	EMS_canonical_SNV	CDS_variant	4	B2,B42,C43,D9
`Chr2:52354139`	`C`	`T`	EMS_canonical_SNV	CDS_variant	5	B2,B43,C43,C71,D9
`Chr2:52354382`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	D10
`Chr2:52355426`	`GC`	`G`	1bp_deletion	frameshift_variant	1	B9
`Chr2:52355426`	`GC`	`G`	1bp_deletion	CDS_variant	1	B9
`Chr2:52355479`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	C41,C83,D3
`Chr2:52355506`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C83
`Chr2:52355545`	`TG`	`T`	1bp_deletion	frameshift_variant	1	C15
`Chr2:52355545`	`TG`	`T`	1bp_deletion	CDS_variant	1	C15
`Chr2:52355710`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B136
`Chr2:52356357`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B107

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057793530.1

470 aa

235

470

Biotin_lipoyl

E3_binding

2-oxoacid_dh

PF00364 Biotin_lipoyl 42-113 aa PF02817 E3_binding 186-221 aa PF00198 2-oxoacid_dh 251-469 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057793530.1`	Pfam	`PF00364`	Biotin_lipoyl	Domain	42	113	8e-16	58.4	Biotin-requiring enzyme
`XP_057793530.1`	Pfam	`PF02817`	E3_binding	Family	186	221	2.4e-15	57.2	e3 binding domain
`XP_057793530.1`	Pfam	`PF00198`	2-oxoacid_dh	Domain	251	469	1.4e-62	211.9	2-oxoacid dehydrogenases acyltransferase (catalytic domain)

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

1 records

下载 cDNA FASTA

XM_057937547.1
AGTTCGATATTAATATAAATGAACGAAAATAAAAAAAATATATAAAAAAAAATTGAATACAGTTCACACTCCAGTTCTCATCAGTATTTCTTGACAATGCCGGTAAAGTTGGTTGAGCCTAGGAAGCTCCGCCTCCTTCCTTCGATGCGTTCAAGGAAACCACTCTCTTTTTTTTAACAACAAATACTCTAACACCCATTTCATCAAACATTTTCTCTGCCTCCTCCCCCTCTCTCTCTCCCCAATTCTCTCGCGCTAAA...

CDS

1 records

下载 CDS FASTA

LOC131010136
ATGTCTCATTCCCACCTCCTCCACACTTCTTTCGTCCCCACCGCTCCTCCCTCCCTCCGCCGCCGTCTCGGCGGCGCAGCCTATCTCCCCCGAAAGCCCCATCTGATTCAATCCAAGATCCGGGAAATCTTCATGCCGGCACTCAGTTCCACGATGACGGAGGGGAAAATCGTTTCCTGGGTCAAATCGGAGGGGGATAAGCTGGCCAAGGGCGAATCAGTCGTGGTGGTCGAGTCCGACAAGGCAGATATGGATGTGGA...

Protein

1 records

下载 Protein FASTA

XP_057793530.1
MSHSHLLHTSFVPTAPPSLRRRLGGAAYLPRKPHLIQSKIREIFMPALSSTMTEGKIVSWVKSEGDKLAKGESVVVVESDKADMDVESFYDGYLAAIIVDEGLSAAVGSTIALLAETEEEIALAQSKKSSSPTSAPVSAASNDAPKVVDEMPSPVPSAAPAVASASSNAGAVGSAVHPASEGGKRVVASPYAKKLAKDLGVDLKGIVGSGPNGRVVAKDVEAALAAVNLGAATTTAAAAAAKPSSVELGSVVPFTTMQSA...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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