Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr02G003645`

染色体: Chr02 坐标: 57804805-57823021 链方向: - 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr02G003645`
名称	SmilChr02G003645
描述	isoamylase 3, chloroplastic
染色体 / SeqID	Chr02 / NC_080388.1
坐标与链方向	57804805 - 57823021 / `-`

ID 对照

NCBI Gene ID	`LOC131010602`
GeneID	`131010602`
locus_tag	`-`
NCBI Name	`LOC131010602`
原始查询	`SmilChr02G003645`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	isoamylase 3, chloroplastic
产物	isoamylase 3%2C chloroplastic
Arabidopsis	`AT4G09020` (ISA3) isoamylase 3 [Source:NCBI gene (formerly Entrezgene);Acc:826481] identity=79.4; qcov=0.992; evalue=0; confidence=high
Rice	`Os08g0520900` (OsPHS8) - identity=46.5; qcov=1.05; evalue=0; confidence=high
GO	GO:0004553 (hydrolase activity, hydrolyzing O-glycosyl compounds); GO:0005975 (carbohydrate metabolic process)
KEGG	smil00500 (Starch and sucrose metabolism - Salvia miltiorrhiza (redroot sage)); smil01100 (Metabolic pathways - Salvia miltiorrhiza (redroot sage)); smil01110 (Biosynthesis of secondary metabolites - Salvia miltiorrhiza (redroot sage))
Pfam	PF00128 (Alpha-amylase); PF02922 (CBM_48); PF21156 (ISOA1-3_C); PF21331 (Isoamylase_C)
InterPro	-
eggNOG	-
NR	GeneID:131010602

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Chloroplast	叶绿体	1.00000	`XP_057794181.1`
2	Cytosol	细胞质基质	1.00000	`XP_057794183.1`
3	Chloroplast stroma	叶绿体基质	1.00000	`XP_057794181.1`
4	Cytoplasm	细胞质	0.80000	`XP_057794183.1`
5	Nucleus	细胞核	0.00000	`XP_057794181.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr2:57805068`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C71
`Chr2:57805104`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B103,B104
`Chr2:57805115`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C71
`Chr2:57806351`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C46
`Chr2:57807623`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B55
`Chr2:57807688`	`G`	`A`	EMS_canonical_SNV	CDS_variant	5	B120,B18,B22,B98,C41
`Chr2:57809677`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B103,B104
`Chr2:57811766`	`C`	`T`	EMS_canonical_SNV	CDS_variant	5	B120,B18,B22,B98,C41
`Chr2:57811770`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	D10
`Chr2:57812444`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C8
`Chr2:57812458`	`G`	`A`	EMS_canonical_SNV	CDS_variant	3	A9,C75,D1
`Chr2:57814976`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C71

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057794181.1

770 aa

385

770

CBM_48

Alpha-amylase

ISOA1-3_C

Isoamylase_C

PF02922 CBM_48 96-194 aa PF00128 Alpha-amylase 264-440 aa PF21156 ISOA1-3_C 682-748 aa PF21331 Isoamylase_C 683-765 aa

XP_057794183.1

616 aa

308

616

Alpha-amylase

ISOA1-3_C

Isoamylase_C

PF00128 Alpha-amylase 110-286 aa PF21156 ISOA1-3_C 528-594 aa PF21331 Isoamylase_C 529-611 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057794181.1`	Pfam	`PF02922`	CBM_48	Domain	96	194	1.9e-12	48.1	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
`XP_057794181.1`	Pfam	`PF00128`	Alpha-amylase	Domain	264	440	2.1e-18	67.4	Alpha amylase, catalytic domain
`XP_057794181.1`	Pfam	`PF21156`	ISOA1-3_C	Domain	682	748	4.4e-14	53.3	Isoamylase 1-3, C-terminal
`XP_057794181.1`	Pfam	`PF21331`	Isoamylase_C	Domain	683	765	2.6e-07	31.8	Isoamylase, C-terminal
`XP_057794183.1`	Pfam	`PF00128`	Alpha-amylase	Domain	110	286	8.4e-19	68.8	Alpha amylase, catalytic domain
`XP_057794183.1`	Pfam	`PF21156`	ISOA1-3_C	Domain	528	594	2.9e-14	53.9	Isoamylase 1-3, C-terminal
`XP_057794183.1`	Pfam	`PF21331`	Isoamylase_C	Domain	529	611	2e-07	32.2	Isoamylase, C-terminal

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

3 records

下载 cDNA FASTA

XR_009096622.1
TCACGTAGGAAATAAGAAAATGAAAATATCTTAAGAGTAAAAACAAAAAAGGAGTTGATAGAAGAGACGTGCCATAAAGATTACAGAGGCCCCAAATCCATACCGTTCGTTCACGTGGCAGACATGAAACGTCTCCTCTTCTACGCAGCTCTAACTTCCATATACACTCACATGCAATTTCCCATTTCTCTCTCTCTCTCTCTCTTGCAAGCCGTGAAATCGGAATTCGTGATTCGTTGATGATAATGATCAGATTACGC...

XM_057938198.1
TCACGTAGGAAATAAGAAAATGAAAATATCTTAAGAGTAAAAACAAAAAAGGAGTTGATAGAAGAGACGTGCCATAAAGATTACAGAGGCCCCAAATCCATACCGTTCGTTCACGTGGCAGACATGAAACGTCTCCTCTTCTACGCAGCTCTAACTTCCATATACACTCACATGCAATTTCCCATTTCTCTCTCTCTCTCTCTCTTGCAAGCCGTGAAATCGGAATTCGTGATTCGTTGATGATAATGATCAGATTACGC...

CDS

2 records

下载 CDS FASTA

LOC131010602
ATGACGCTTCAGCTTTCCACTACTCTTTGGAGCAACGGCAATTCCGCTCTCGCCTCCCGCCCAACTTCCGTCCGTTGCAGACCGGAATCAAGTGATAGAGCGTCGCACTTGAAAGCTTGCCAGCGGGGAGTTTTCAGTGAGGTCAGACAAGATTTCGGTGGAAGTCGCCGAATGGGTTCATCTGTGCACGCAACTCGAGCTGGTGAGCAGAGTGTGGCGATAGAGGAACAAGCACTCCAGAAAACAGAGGGTCCCTTGCT...

LOC131010602
ATGCAAAAAAAAGGTTTGCCACGTAGCAATGTCTTATATGGTTACCGTGTTGATGGCCCTCGAGATTGGCGTGAAGGACATCGCTTTGATTACAACAATGTTTTACTTGATCCATATGCAAAATTGGTTGAAGGACGCCGAGTCTTCGGTGATGCTAGCAATAAGATGTCTAAATTTTTTGGGACATATGATTTTGATAGCTTGCCTTTTGACTGGGGAGAGAACTATGAGCTTCCAAATATACATGAGAAAGATCTTGT...

Protein

2 records

下载 Protein FASTA

XP_057794181.1
MTLQLSTTLWSNGNSALASRPTSVRCRPESSDRASHLKACQRGVFSEVRQDFGGSRRMGSSVHATRAGEQSVAIEEQALQKTEGPLLKVFPGQSHPLGVSEVETGTNFAIFSENATTVTLCLILQERGMHNKLEEGMVELSLDSQVNKTGDIWHICVEGLPRSNVLYGYRVDGPRDWREGHRFDYNNVLLDPYAKLVEGRRVFGDASNKMSKFFGTYDFDSLPFDWGENYELPNIHEKDLVLYEMNVRAFTADESSGLDL...

XP_057794183.1
MQKKGLPRSNVLYGYRVDGPRDWREGHRFDYNNVLLDPYAKLVEGRRVFGDASNKMSKFFGTYDFDSLPFDWGENYELPNIHEKDLVLYEMNVRAFTADESSGLDLHVRGSYLGMIEKIPHLLELGVNAVELLPVFEFDEMELQRRPNAREHLINTWGYSTVNFFAPMSRYASNGGGPINASQEFKQMVKAFHDAGIEVILDVVYNHTNEADDAHPYTTSFRGIDNKVYYMMDFQQNAQLLNFSGCGNTLNCNHPVVMEL...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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