Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr02G004430`

染色体: Chr02 坐标: 66646396-66652263 链方向: - 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr02G004430`
名称	SmilChr02G004430
描述	NADP-dependent malic enzyme-like
染色体 / SeqID	Chr02 / NC_080388.1
坐标与链方向	66646396 - 66652263 / `-`

ID 对照

NCBI Gene ID	`LOC131013181`
GeneID	`131013181`
locus_tag	`-`
NCBI Name	`LOC131013181`
原始查询	`SmilChr02G004430`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	NADP-dependent malic enzyme-like
产物	NADP-dependent malic enzyme-like
Arabidopsis	`AT1G79750` (NADP-ME4) NADP-malic enzyme 4 [Source:NCBI gene (formerly Entrezgene);Acc:844314] identity=76.3; qcov=1; evalue=0; confidence=high
Rice	`Os01g0723400` (NADP-ME2) - identity=79.5; qcov=0.92; evalue=0; confidence=high
GO	GO:0004471 (malate dehydrogenase (decarboxylating) (NAD+) activity); GO:0004473 (malate dehydrogenase (decarboxylating) (NADP+) activity); GO:0006090 (pyruvate metabolic process); GO:0006108 (malate metabolic process); GO:0009507 (chloroplast); GO:0051287 (NAD binding)
KEGG	smil00620 (Pyruvate metabolism - Salvia miltiorrhiza (redroot sage)); smil00710 (Carbon fixation by Calvin cycle - Salvia miltiorrhiza (redroot sage)); smil01100 (Metabolic pathways - Salvia miltiorrhiza (redroot sage)); smil01200 (Carbon metabolism - Salvia miltiorrhiza (redroot sage))
Pfam	PF00390 (malic); PF03949 (Malic_M)
InterPro	-
eggNOG	-
NR	GeneID:131013181

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Chloroplast	叶绿体	1.00000	`XP_057797217.1`
2	Chloroplast stroma	叶绿体基质	1.00000	`XP_057797217.1`
3	Nucleus	细胞核	0.00000	`XP_057797217.1`
4	Chromosome	染色体	0.00000	`XP_057797217.1`
5	Centromere	着丝粒	0.00000	`XP_057797217.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr2:66646621`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B129
`Chr2:66646644`	`G`	`A`	EMS_canonical_SNV	CDS_variant	3	B115,C45,C83
`Chr2:66646683`	`C`	`T`	EMS_canonical_SNV	CDS_variant	5	A19,B111,B118,B15,B45
`Chr2:66646840`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B84
`Chr2:66647879`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B36,C10
`Chr2:66647989`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B104
`Chr2:66648387`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C5
`Chr2:66648859`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B122,C30
`Chr2:66649403`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B131,B95,C14
`Chr2:66649661`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C2
`Chr2:66649663`	`C`	`T`	EMS_canonical_SNV	CDS_variant	5	A21,A24,B53,B71,C45
`Chr2:66650117`	`G`	`A`	EMS_canonical_SNV	CDS_variant	4	A21,B53,B71,C45

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057797217.1

653 aa

326

653

malic

Malic_M

PF00390 malic 178-358 aa PF03949 Malic_M 369-622 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057797217.1`	Pfam	`PF00390`	malic	Domain	178	358	1.4e-78	263.6	Malic enzyme, N-terminal domain
`XP_057797217.1`	Pfam	`PF03949`	Malic_M	Domain	369	622	9.7e-97	324.0	Malic enzyme, NAD binding domain

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

1 records

下载 cDNA FASTA

XM_057941234.1
AAGAAACAGTGAAGAAAGAGAAGTAGTCATAAAGCTTGCGCTGCTTCCCTGGTATATAGCTAACTCGGAGGAGAGAGAAAGGCAGAGCGAGAGAGATAGAGAGTGGGAGGGAGATCGCGACGCGAGCCAGGCGATCGCATAAAACGGAGCTACTCTCACCACACTCTCTTTCTCTTTCCTCTCATTTTCTCCTTCTCTGCACTCATCACACCTACAGACCAAAAACACTCGCCTCAAATCTTCCGCCACCATGTTCTCAT...

CDS

1 records

下载 CDS FASTA

LOC131013181
ATGTTCTCATTGAATGGAAGCAGTTTTCTGAATAATTCTCTCTCGGGGGTGTCGAAGAGCTTGACGCAATCTTCACAGAGGAAGATATCAGCTCCAAGTGTGGTGGTGGCTTCTGTGAGCTCAAATGGCAGGCCTGCAGACCGAAACGTCAGCGTTTTGATGGAGAATACTCTGAAGGAGATGAGAGAGAATGCTCCCTCCTCCTCATCACCATCGCAGTCGCAGGTGACGGAGATTGAACCAGCATCCACCGTCGGCGG...

Protein

1 records

下载 Protein FASTA

XP_057797217.1
MFSLNGSSFLNNSLSGVSKSLTQSSQRKISAPSVVVASVSSNGRPADRNVSVLMENTLKEMRENAPSSSSPSQSQVTEIEPASTVGGGVEDVYGEDTATEDQYITPWTVTVASGYPLLRDPHYNKGLAFTLKERDAHFLRGLLPPVVVSQELQVKKMMANMRQYQVPLQRYMAMMDLQERNERLFYKLLIENVEELLPVVYTPTVGEACQKYGSIFRQPQGLFISLKEKGRILEVLRNWPEKKIQVIVVTDGERILGLGD...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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JBrowse

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