Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr02G004617`

染色体: Chr02 坐标: 68679256-68684499 链方向: - 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr02G004617`
名称	SmilChr02G004617
描述	E3 ubiquitin-protein ligase ORTHRUS 2-like
染色体 / SeqID	Chr02 / NC_080388.1
坐标与链方向	68679256 - 68684499 / `-`

ID 对照

NCBI Gene ID	`LOC131008577`
GeneID	`131008577`
locus_tag	`-`
NCBI Name	`LOC131008577`
原始查询	`SmilChr02G004617`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	E3 ubiquitin-protein ligase ORTHRUS 2-like
产物	E3 ubiquitin-protein ligase ORTHRUS 2-like
Arabidopsis	`AT1G57820` (VIM1) Zinc finger (C3HC4-type RING finger) family protein [Source:NCBI gene (formerly Entrezgene);Acc:842157] identity=57.3; qcov=0.958; evalue=0; confidence=high
Rice	`Os05g0102600` (OsRFPHC-11) - identity=53.2; qcov=1.01; evalue=0; confidence=high
GO	GO:0016567 (protein ubiquitination); GO:0044027 (negative regulation of gene expression via chromosomal CpG island methylation); GO:0046872 (metal ion binding); GO:0061630 (ubiquitin protein ligase activity)
KEGG	-
Pfam	PF00097 (zf-C3HC4); PF02182 (SAD_SRA); PF13445 (zf-RING_UBOX); PF13639 (zf-RING_2); PF13920 (zf-C3HC4_3); PF13923 (zf-C3HC4_2)
InterPro	-
eggNOG	-
NR	GeneID:131008577

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Nucleus	细胞核	1.00000	`XP_057791489.1`
2	Chromosome	染色体	0.00000	`XP_057791489.1`
3	Centromere	着丝粒	0.00000	`XP_057791489.1`
4	Chromatin	染色质	0.00000	`XP_057791489.1`
5	Telomere	端粒	0.00000	`XP_057791489.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr2:68679642`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C37
`Chr2:68679681`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	D4
`Chr2:68679784`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B2
`Chr2:68679808`	`T`	`TTC`	2bp_insertion	frameshift_variant	5	A23,B121,B57,C10,C60
`Chr2:68679808`	`T`	`TTC`	2bp_insertion	CDS_variant	5	A23,B121,B57,C10,C60
`Chr2:68679810`	`TAG`	`T`	2bp_deletion	frameshift_variant	5	A23,B121,B57,C10,C60
`Chr2:68679810`	`TAG`	`T`	2bp_deletion	CDS_variant	5	A23,B121,B57,C10,C60
`Chr2:68679862`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B33,B75,C42
`Chr2:68680068`	`CCT`	`C`	2bp_deletion	frameshift_variant	3	B47,C56,C65
`Chr2:68680068`	`CCT`	`C`	2bp_deletion	CDS_variant	3	B47,C56,C65
`Chr2:68680536`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B97
`Chr2:68680947`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B93,C2

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057791489.1

668 aa

334

668

zf-C3HC4_3

zf-C3HC4_2

zf-RING_2

zf-C3HC4

zf-RING_UBOX

SAD_SRA

zf-RING_UBOX

zf-C3HC4

PF13920 zf-C3HC4_3 128-172 aa PF13923 zf-C3HC4_2 129-168 aa PF13639 zf-RING_2 129-169 aa PF00097 zf-C3HC4 130-168 aa PF13445 zf-RING_UBOX 130-168 aa PF02182 SAD_SRA 262-409 aa PF13445 zf-RING_UBOX 502-534 aa PF00097 zf-C3HC4 502-542 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057791489.1`	Pfam	`PF13920`	zf-C3HC4_3	Domain	128	172	3.6e-08	33.8	Zinc finger, C3HC4 type (RING finger)
`XP_057791489.1`	Pfam	`PF13923`	zf-C3HC4_2	Domain	129	168	1e-09	38.7	Zinc finger, C3HC4 type (RING finger)
`XP_057791489.1`	Pfam	`PF13639`	zf-RING_2	Domain	129	169	7.4e-09	36.5	Ring finger domain
`XP_057791489.1`	Pfam	`PF00097`	zf-C3HC4	Domain	130	168	6e-11	42.7	Zinc finger, C3HC4 type (RING finger)
`XP_057791489.1`	Pfam	`PF13445`	zf-RING_UBOX	Domain	130	168	4.1e-10	40.2	RING-type zinc-finger
`XP_057791489.1`	Pfam	`PF02182`	SAD_SRA	Domain	262	409	5.5e-49	166.4	SAD/SRA domain
`XP_057791489.1`	Pfam	`PF13445`	zf-RING_UBOX	Domain	502	534	4.1e-10	40.2	RING-type zinc-finger
`XP_057791489.1`	Pfam	`PF00097`	zf-C3HC4	Domain	502	542	6e-11	42.7	Zinc finger, C3HC4 type (RING finger)

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

1 records

下载 cDNA FASTA

XM_057935506.1
AATTTATTCTCTTTCTCGCTTTAAGACAGTTGTTTCACTCTCTCTCTCCAGGCAAGCAACAACTATCGCGGCAGTCTGTAAAATGGCGCACCAACTCCCTCTCGATAGCGACGGCGTCTGCATGCTCTGTAAAGAGAAGCCGTCGGAGGACGCCACCCTCTGCTGCGGAACGTGTGCCACGCCATGGCACGTGGCCTGCCTTTCTGGGATAAAAAATTCCGCCCTAGGGTTCGAGTGCCCCGACTGCTCCGGCGATGGGC...

CDS

1 records

下载 CDS FASTA

LOC131008577
ATGGCGCACCAACTCCCTCTCGATAGCGACGGCGTCTGCATGCTCTGTAAAGAGAAGCCGTCGGAGGACGCCACCCTCTGCTGCGGAACGTGTGCCACGCCATGGCACGTGGCCTGCCTTTCTGGGATAAAAAATTCCGCCCTAGGGTTCGAGTGCCCCGACTGCTCCGGCGATGGGCTCGGCGGAGCTCCGGCGCCCACTGACTTGGACAAGAAAGATCTGTTTTCCAAAATCCGGGCGGTCGAAGCCGATGATTCTTT...

Protein

1 records

下载 Protein FASTA

XP_057791489.1
MAHQLPLDSDGVCMLCKEKPSEDATLCCGTCATPWHVACLSGIKNSALGFECPDCSGDGLGGAPAPTDLDKKDLFSKIRAVEADDSLTDVEKARKRQQLMCGKIESGEASGVKEENSCDILAVIGESFKCSYCMELPERPVTTPCGHNFCLKCFEKWIKQGKRTCIKCRSNIPNRMASRPHINSTLVAAIRMAKLSRTITSGGPQTVYRYLHDQDRPDTAFTTERAQRAGMANAASGRIFVTLPKDHFGPITAENDPERN...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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