Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr02G004922`

染色体: Chr02 坐标: 71241643-71243128 链方向: - 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr02G004922`
名称	SmilChr02G004922
描述	malate dehydrogenase, chloroplastic
染色体 / SeqID	Chr02 / NC_080388.1
坐标与链方向	71241643 - 71243128 / `-`

ID 对照

NCBI Gene ID	`LOC131008965`
GeneID	`131008965`
locus_tag	`-`
NCBI Name	`LOC131008965`
原始查询	`SmilChr02G004922`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	malate dehydrogenase, chloroplastic
产物	malate dehydrogenase%2C chloroplastic
Arabidopsis	`AT3G47520` (MDH) malate dehydrogenase [Source:NCBI gene (formerly Entrezgene);Acc:823906] identity=77.2; qcov=0.917; evalue=0; confidence=high
Rice	`Os08g0434300` (MDH) - identity=78.6; qcov=0.92; evalue=0; confidence=high
GO	GO:0005737 (cytoplasm); GO:0005739 (mitochondrion); GO:0006099 (tricarboxylic acid cycle); GO:0006108 (malate metabolic process); GO:0030060 (L-malate dehydrogenase (NAD+) activity)
KEGG	smil00020 (Citrate cycle (TCA cycle) - Salvia miltiorrhiza (redroot sage)); smil00270 (Cysteine and methionine metabolism - Salvia miltiorrhiza (redroot sage)); smil00566 (Sulfoquinovose metabolism - Salvia miltiorrhiza (redroot sage)); smil00620 (Pyruvate metabolism - Salvia miltiorrhiza (redroot sage)); smil00630 (Glyoxylate and dicarboxylate metabolism - Salvia miltiorrhiza (redroot sage)); smil00710 (Carbon fixation by Calvin cycle - Salvia miltiorrhiza (redroot sage)); smil01100 (Metabolic pathways - Salvia miltiorrhiza (redroot sage)); smil01110 (Biosynthesis of secondary metabolites - Salvia miltiorrhiza (redroot sage)); smil01200 (Carbon metabolism - Salvia miltiorrhiza (redroot sage))
Pfam	PF00056 (Ldh_1_N); PF02866 (Ldh_1_C)
InterPro	-
eggNOG	-
NR	GeneID:131008965

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Chloroplast	叶绿体	1.00000	`XP_057792099.1`
2	Chloroplast stroma	叶绿体基质	1.00000	`XP_057792099.1`
3	Nucleus	细胞核	0.00000	`XP_057792099.1`
4	Chromosome	染色体	0.00000	`XP_057792099.1`
5	Centromere	着丝粒	0.00000	`XP_057792099.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr2:71242076`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B29,C82
`Chr2:71242085`	`G`	`A`	EMS_canonical_SNV	CDS_variant	5	B118,B35,C70,C72,D10
`Chr2:71242134`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B26
`Chr2:71242187`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C35
`Chr2:71242211`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B104
`Chr2:71242273`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C28
`Chr2:71242370`	`C`	`T`	EMS_canonical_SNV	CDS_variant	5	B104,B90,B92,C21,C73
`Chr2:71242525`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B72
`Chr2:71242647`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C28
`Chr2:71242649`	`G`	`A`	EMS_canonical_SNV	CDS_variant	3	B24,B33,C81
`Chr2:71242758`	`TA`	`T`	1bp_deletion	frameshift_variant	2	B132,D4
`Chr2:71242758`	`TA`	`T`	1bp_deletion	CDS_variant	2	B132,D4

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057792099.1

411 aa

206

411

Ldh_1_N

Ldh_1_C

PF00056 Ldh_1_N 94-236 aa PF02866 Ldh_1_C 238-404 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057792099.1`	Pfam	`PF00056`	Ldh_1_N	Domain	94	236	3.8e-48	163.8	lactate/malate dehydrogenase, NAD binding domain
`XP_057792099.1`	Pfam	`PF02866`	Ldh_1_C	Domain	238	404	8.2e-44	150.4	lactate/malate dehydrogenase, alpha/beta C-terminal domain

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

1 records

下载 cDNA FASTA

XM_057936116.1
ATGGCCGCAACATCAGCAACCAGTTTTTCTGTCGGATCGACTGCATCTGCTGGGCCCAAAGTTGGCTTCACGCAGTCAAAGGCCTTTAGCGTGAAGTTGAACCCAAAGAAGTACCTAACGAGCTTCAGTGGCCTCAAGGCAGCAAACTCCGTGAGCTGTGAATCACAATCGTCGTTCTTAGGCAGTGAAAGCACTCTAGCTCTTAAGCAGGGTTATAATTCGAAGGCCCGGAAATGCGGTGAGAGGTCGTACTGTCATGT...

CDS

1 records

下载 CDS FASTA

LOC131008965
ATGGCCGCAACATCAGCAACCAGTTTTTCTGTCGGATCGACTGCATCTGCTGGGCCCAAAGTTGGCTTCACGCAGTCAAAGGCCTTTAGCGTGAAGTTGAACCCAAAGAAGTACCTAACGAGCTTCAGTGGCCTCAAGGCAGCAAACTCCGTGAGCTGTGAATCACAATCGTCGTTCTTAGGCAGTGAAAGCACTCTAGCTCTTAAGCAGGGTTATAATTCGAAGGCCCGGAAATGCGGTGAGAGGTCGTACTGTCATGT...

Protein

1 records

下载 Protein FASTA

XP_057792099.1
MAATSATSFSVGSTASAGPKVGFTQSKAFSVKLNPKKYLTSFSGLKAANSVSCESQSSFLGSESTLALKQGYNSKARKCGERSYCHVQPQASYKVAILGASGGIGQPLALLIKMSPLVSALNLYDIANVKGVAADLSHCNTPSQVSDFTGESELAACLRDADVVVIPAGVPRKPGMTRDDLFNINANIVKNLVGAVADNCPDAFIHIISNPVNSTVPIAAEVLKQKGVYDPKKLFGVTTLDVVRANTFVAQKKNLKLIDV...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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