Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr02G005218`

染色体: Chr02 坐标: 73387761-73394913 链方向: + 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr02G005218`
名称	SmilChr02G005218
描述	glutamate--cysteine ligase, chloroplastic-like
染色体 / SeqID	Chr02 / NC_080388.1
坐标与链方向	73387761 - 73394913 / `+`

ID 对照

NCBI Gene ID	`LOC131009216`
GeneID	`131009216`
locus_tag	`-`
NCBI Name	`LOC131009216`
原始查询	`SmilChr02G005218`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	glutamate--cysteine ligase, chloroplastic-like
产物	glutamate--cysteine ligase%2C chloroplastic-like
Arabidopsis	`AT4G23100` (GSH1) glutamate-cysteine ligase [Source:NCBI gene (formerly Entrezgene);Acc:828409] identity=76.1; qcov=1; evalue=0; confidence=high
Rice	`Os05g0129000` (OsECS) - identity=79.6; qcov=0.867; evalue=0; confidence=high
GO	GO:0004357 (glutamate-cysteine ligase activity); GO:0006750 (glutathione biosynthetic process); GO:0042398 (modified amino acid biosynthetic process)
KEGG	smil00270 (Cysteine and methionine metabolism - Salvia miltiorrhiza (redroot sage)); smil00340 (Histidine metabolism - Salvia miltiorrhiza (redroot sage)); smil00480 (Glutathione metabolism - Salvia miltiorrhiza (redroot sage)); smil01100 (Metabolic pathways - Salvia miltiorrhiza (redroot sage)); smil01110 (Biosynthesis of secondary metabolites - Salvia miltiorrhiza (redroot sage)); smil01240 (Biosynthesis of cofactors - Salvia miltiorrhiza (redroot sage))
Pfam	PF04107 (GCS2)
InterPro	-
eggNOG	-
NR	GeneID:131009216

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Chloroplast	叶绿体	1.00000	`XP_057792454.1`
2	Chloroplast stroma	叶绿体基质	1.00000	`XP_057792454.1`
3	Nucleus	细胞核	0.00000	`XP_057792454.1`
4	Chromosome	染色体	0.00000	`XP_057792454.1`
5	Centromere	着丝粒	0.00000	`XP_057792454.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr2:73388808`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B107
`Chr2:73388873`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C72
`Chr2:73388874`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C53
`Chr2:73388951`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B16
`Chr2:73388961`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B118,B26
`Chr2:73389382`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	A1,A10,C53
`Chr2:73390390`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C53
`Chr2:73390423`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C53
`Chr2:73390435`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C53
`Chr2:73390438`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C53
`Chr2:73391129`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C53
`Chr2:73391167`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B107,B6,D1

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057792454.1

526 aa

263

526

GCS2

PF04107 GCS2 113-522 aa

XP_057792455.1

526 aa

263

526

GCS2

PF04107 GCS2 113-522 aa

XP_057792456.1

526 aa

263

526

GCS2

PF04107 GCS2 113-522 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057792454.1`	Pfam	`PF04107`	GCS2	Family	113	522	3.2e-108	363.1	Glutamate-cysteine ligase family 2(GCS2)
`XP_057792455.1`	Pfam	`PF04107`	GCS2	Family	113	522	3.2e-108	363.1	Glutamate-cysteine ligase family 2(GCS2)
`XP_057792456.1`	Pfam	`PF04107`	GCS2	Family	113	522	3.2e-108	363.1	Glutamate-cysteine ligase family 2(GCS2)

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

3 records

下载 cDNA FASTA

XM_057936471.1
ATGGAATGGACCACAGCAAAAAGAGTGGACTCTGCCGCACATCATAGAACCCCTACGCCATTCTTTTCCTCTTTTGGGTTTCTGCTCACTAAATTCTACTGTTCAATCACGCCATTTCCATTTCGTGCTTCTGATTCTGTTTATCTCAAGCCGATTAAAGCTGCCAGCCATGGCATTGATGTCCCATCCTGGTTCATCTCATAGCATTCAGTCAGAGGTTATACAGTGTAAAACTAGGCACAATGGAGTTAATAGCTTTG...

XM_057936472.1
ATGGAATGGACCACAGCAAAAAGAGTGGACTCTGCCGCACATCATAGAACCCCTACGCCATTCTTTTCCTCTTTTGGGTTTCTGCTCACTAAATTCTACTGTTCAATCACGCCATTTCCATTTCGTGCTTCTGATTCTGTTTATCTCAAGCCGATTAAAGCTGCCAGCCATGGCATTGATGTCCCATCCTGGTTCATCTCATAGCATTCAGTCAGAGGTTATACAGTGTAAAACTAGGCACAATGGAGTTAATAGCTTTG...

CDS

3 records

下载 CDS FASTA

LOC131009216
ATGGCATTGATGTCCCATCCTGGTTCATCTCATAGCATTCAGTCAGAGGTTATACAGTGTAAAACTAGGCACAATGGAGTTAATAGCTTTGCCAACACAGTTGACTCATCCAGGCCAAAGGAAACCTTCTTAGGTCTGCGATCCCCTTCGTGGAGTTCCCTGAAGGTGAAACCAAGCTCGAGTTCTGTTGGCACTGGAGTGGGAAGGAGGCGAGGGCGCCAGGAAACTGTTGCCGCAAGTCCTCCTACAGACGATGCTGT...

Protein

3 records

下载 Protein FASTA

XP_057792454.1
MALMSHPGSSHSIQSEVIQCKTRHNGVNSFANTVDSSRPKETFLGLRSPSWSSLKVKPSSSSVGTGVGRRRGRQETVAASPPTDDAVISTEPLTKEDLVGYLASGCKPKENWRIGTEHEKFGFEIGTLSPMKYEQIAELLNVISERFDWDKIMEGENIIGLKQDKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPIMPKGRYVIMKNYMPKVGSLGLDMMFRTCTVQVN...

XP_057792455.1
MALMSHPGSSHSIQSEVIQCKTRHNGVNSFANTVDSSRPKETFLGLRSPSWSSLKVKPSSSSVGTGVGRRRGRQETVAASPPTDDAVISTEPLTKEDLVGYLASGCKPKENWRIGTEHEKFGFEIGTLSPMKYEQIAELLNVISERFDWDKIMEGENIIGLKQDKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPIMPKGRYVIMKNYMPKVGSLGLDMMFRTCTVQVN...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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JBrowse

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