Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr03G002649`

染色体: Chr03 坐标: 42141327-42153624 链方向: + 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr03G002649`
名称	SmilChr03G002649
描述	NAD-dependent malic enzyme 62 kDa isoform, mitochondrial
染色体 / SeqID	Chr03 / NC_080389.1
坐标与链方向	42141327 - 42153624 / `+`

ID 对照

NCBI Gene ID	`LOC131015839`
GeneID	`131015839`
locus_tag	`-`
NCBI Name	`LOC131015839`
原始查询	`SmilChr03G002649`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	NAD-dependent malic enzyme 62 kDa isoform, mitochondrial
产物	NAD-dependent malic enzyme 62 kDa isoform%2C mitochondrial
Arabidopsis	`AT2G13560` (NAD-ME1) NAD-dependent malic enzyme 1 [Source:NCBI gene (formerly Entrezgene);Acc:815842] identity=83.1; qcov=0.954; evalue=0; confidence=high
Rice	`Os07g0496200` (OJ1457_D07.117) - identity=79.4; qcov=0.987; evalue=0; confidence=high
GO	GO:0004471 (malate dehydrogenase (decarboxylating) (NAD+) activity); GO:0005739 (mitochondrion); GO:0006090 (pyruvate metabolic process); GO:0006108 (malate metabolic process); GO:0051287 (NAD binding)
KEGG	smil00620 (Pyruvate metabolism - Salvia miltiorrhiza (redroot sage)); smil00710 (Carbon fixation by Calvin cycle - Salvia miltiorrhiza (redroot sage)); smil01100 (Metabolic pathways - Salvia miltiorrhiza (redroot sage)); smil01200 (Carbon metabolism - Salvia miltiorrhiza (redroot sage))
Pfam	PF00390 (malic); PF03949 (Malic_M)
InterPro	-
eggNOG	-
NR	GeneID:131015839

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Mitochondrion	线粒体	1.00000	`XP_057800259.1`
2	Mitochondrion matrix	线粒体基质	0.20000	`XP_057800259.1`
3	Nucleus	细胞核	0.00000	`XP_057800259.1`
4	Chromosome	染色体	0.00000	`XP_057800259.1`
5	Centromere	着丝粒	0.00000	`XP_057800259.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr3:42142456`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	D11
`Chr3:42143712`	`AG`	`A`	1bp_deletion	frameshift_variant	1	C19
`Chr3:42143712`	`AG`	`A`	1bp_deletion	CDS_variant	1	C19
`Chr3:42145086`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B32
`Chr3:42146282`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B58,C31
`Chr3:42148004`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	A15,B11,B89
`Chr3:42149380`	`G`	`A`	EMS_canonical_SNV	CDS_variant	4	B14,B31,C71,D5
`Chr3:42149755`	`TG`	`T`	1bp_deletion	frameshift_variant	1	B39
`Chr3:42149755`	`TG`	`T`	1bp_deletion	CDS_variant	1	B39
`Chr3:42150808`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	D11
`Chr3:42152355`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	A22

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057800259.1

628 aa

314

628

malic

Malic_M

PF00390 malic 125-305 aa PF03949 Malic_M 315-583 aa

XP_057800260.1

628 aa

314

628

malic

Malic_M

PF00390 malic 125-305 aa PF03949 Malic_M 315-583 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057800259.1`	Pfam	`PF00390`	malic	Domain	125	305	3.1e-77	259.2	Malic enzyme, N-terminal domain
`XP_057800259.1`	Pfam	`PF03949`	Malic_M	Domain	315	583	1.1e-88	297.6	Malic enzyme, NAD binding domain
`XP_057800260.1`	Pfam	`PF00390`	malic	Domain	125	305	3.1e-77	259.2	Malic enzyme, N-terminal domain
`XP_057800260.1`	Pfam	`PF03949`	Malic_M	Domain	315	583	1.1e-88	297.6	Malic enzyme, NAD binding domain

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

2 records

下载 cDNA FASTA

XM_057944276.1
ACCTCTACTACCAGAATGAGGTACAACCATTCATGAACAATGTACGACAATGCTTACTCTGGCATTAGCAAAACTGATTTTGTCATGGGCCTTACTGTTCAAAGTTCCCGTCGTATTCAAGCTGTCTATGGAGATATAAATTCCTTCACTACTGTAGCTATACACTCAAGTGGATTGGATTTTTCTTCCTTCTGCTGTTTCTCTGCGTAAATTATGGGGAATTTCTCTCGCCAGATGACGCTTTCGTCAGCGCTGTTGAA...

XM_057944277.1
ACCTCTACTACCAGAATGAGGTACAACCATTCATGAACAATGTACGACAATGCTTACTCTGGCATTAGCAAAACTGATTTTGTCATGGGCCTTACTGTTCAAAGTTCCCGTCGTATTCAAGCTGTCTATGGAGATATAAATTCCTTCACTACTGTAGCTATACACTCAAGTGGATTGGATTTTTCTTCCTTCTGCTGTTTCTCTGCGTAAATTATGGGGAATTTCTCTCGCCAGATGACGCTTTCGTCAGCGCTGTTGAA...

CDS

2 records

下载 CDS FASTA

LOC131015839
ATGGGGAATTTCTCTCGCCAGATGACGCTTTCGTCAGCGCTGTTGAAGCGCTTACAATCGCGGCGGGGAGCTCTCAACGCCGTGAATTCGGGCGGCGCGAGATCTTTTACGACGACCGAGGGCCACCGTCCGACCATAATTCACAAGCGCAGCTTGGATATTCTCCACGATCCGTGGTTCAACAAGGGTACTGCATTTTCAATGACAGAACGCGATCGCCTTGACCTCCGTGGGTTACTGCCTCCAAATGTCATGAGTCC...

Protein

2 records

下载 Protein FASTA

XP_057800259.1
MGNFSRQMTLSSALLKRLQSRRGALNAVNSGGARSFTTTEGHRPTIIHKRSLDILHDPWFNKGTAFSMTERDRLDLRGLLPPNVMSPEQQIERFMADLKRLEQSARDGPSDPYSLAKWRILNRLHDRNETMYYKVLIDNIEEYAPIVYTPTVGLVCQKYSGLFRRPRGMYFSAADRGEMMSMVYNWPAEQVDMIVVTDGSRILGLGDLGVQGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNENLLKDPLYLGLQEH...

XP_057800260.1
MGNFSRQMTLSSALLKRLQSRRGALNAVNSGGARSFTTTEGHRPTIIHKRSLDILHDPWFNKGTAFSMTERDRLDLRGLLPPNVMSPEQQIERFMADLKRLEQSARDGPSDPYSLAKWRILNRLHDRNETMYYKVLIDNIEEYAPIVYTPTVGLVCQKYSGLFRRPRGMYFSAADRGEMMSMVYNWPAEQVDMIVVTDGSRILGLGDLGVQGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNENLLKDPLYLGLQEH...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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