Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr04G002003`

染色体: Chr04 坐标: 32431662-32434851 链方向: - 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr04G002003`
名称	SmilChr04G002003
描述	E3 ubiquitin-protein ligase SIS3
染色体 / SeqID	Chr04 / NC_080390.1
坐标与链方向	32431662 - 32434851 / `-`

ID 对照

NCBI Gene ID	`LOC131019968`
GeneID	`131019968`
locus_tag	`-`
NCBI Name	`LOC131019968`
原始查询	`SmilChr04G002003`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	E3 ubiquitin-protein ligase SIS3
产物	E3 ubiquitin-protein ligase SIS3
Arabidopsis	`AT3G47990` (SIS3) SUGAR-INSENSITIVE 3 [Source:NCBI gene (formerly Entrezgene);Acc:823954] identity=75.1; qcov=0.956; evalue=0; confidence=high
Rice	`Os05g0145000` (PTB1) - identity=77.4; qcov=0.981; evalue=0; confidence=high
GO	GO:0004842 (ubiquitin-protein transferase activity); GO:0010182 (sugar mediated signaling pathway)
KEGG	-
Pfam	PF00097 (zf-C3HC4); PF13445 (zf-RING_UBOX); PF13639 (zf-RING_2); PF13923 (zf-C3HC4_2); PF17123 (zf-RING_11); PF26753 (SIS3_N)
InterPro	-
eggNOG	-
NR	GeneID:131019968

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Endosome	内体	1.00000	`XP_057804590.1`
2	Endosome membrane	内体膜	1.00000	`XP_057804590.1`
3	Lysosome membrane	溶酶体膜	0.60000	`XP_057804591.1`
4	Cytoplasmic vesicle	细胞质囊泡	0.40000	`XP_057804590.1`
5	Lysosome	溶酶体	0.40000	`XP_057804591.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr4:32431939`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B128,C18
`Chr4:32431940`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B44,C33,D11
`Chr4:32431958`	`C`	`T`	EMS_canonical_SNV	CDS_variant	5	B14,B15,B36,C33,C71
`Chr4:32432021`	`G`	`A`	EMS_canonical_SNV	CDS_variant	3	B126,B133,B94
`Chr4:32432144`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B41
`Chr4:32432206`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B130
`Chr4:32432389`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	C73,C9
`Chr4:32432401`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B94
`Chr4:32432481`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	C32,C68
`Chr4:32432520`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B108,C85
`Chr4:32433836`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	A20,C54,C86
`Chr4:32434513`	`C`	`T`	EMS_canonical_SNV	CDS_variant	4	B125,B90,B92,C87

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057804590.1

388 aa

194

388

SIS3_N

zf-RING_11

zf-C3HC4_2

zf-RING_2

zf-RING_UBOX

zf-C3HC4

PF26753 SIS3_N 11-161 aa PF17123 zf-RING_11 261-289 aa PF13923 zf-C3HC4_2 261-302 aa PF13639 zf-RING_2 261-303 aa PF13445 zf-RING_UBOX 262-300 aa PF00097 zf-C3HC4 262-302 aa

XP_057804591.1

361 aa

180

361

SIS3_N

zf-RING_11

zf-C3HC4_2

zf-RING_2

zf-RING_UBOX

zf-C3HC4

PF26753 SIS3_N 11-161 aa PF17123 zf-RING_11 234-262 aa PF13923 zf-C3HC4_2 234-275 aa PF13639 zf-RING_2 234-276 aa PF13445 zf-RING_UBOX 235-273 aa PF00097 zf-C3HC4 235-275 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057804590.1`	Pfam	`PF26753`	SIS3_N	Domain	11	161	2.2e-69	232.8	SIS3, N-terminal domain
`XP_057804590.1`	Pfam	`PF17123`	zf-RING_11	Domain	261	289	9.7e-11	41.9	RING-like zinc finger
`XP_057804590.1`	Pfam	`PF13923`	zf-C3HC4_2	Domain	261	302	7e-09	36.1	Zinc finger, C3HC4 type (RING finger)
`XP_057804590.1`	Pfam	`PF13639`	zf-RING_2	Domain	261	303	2.3e-14	54.1	Ring finger domain
`XP_057804590.1`	Pfam	`PF13445`	zf-RING_UBOX	Domain	262	300	5.4e-08	33.4	RING-type zinc-finger
`XP_057804590.1`	Pfam	`PF00097`	zf-C3HC4	Domain	262	302	5e-08	33.4	Zinc finger, C3HC4 type (RING finger)
`XP_057804591.1`	Pfam	`PF26753`	SIS3_N	Domain	11	161	1.9e-69	233.0	SIS3, N-terminal domain
`XP_057804591.1`	Pfam	`PF17123`	zf-RING_11	Domain	234	262	8.8e-11	42.0	RING-like zinc finger
`XP_057804591.1`	Pfam	`PF13923`	zf-C3HC4_2	Domain	234	275	6.3e-09	36.2	Zinc finger, C3HC4 type (RING finger)
`XP_057804591.1`	Pfam	`PF13639`	zf-RING_2	Domain	234	276	2.1e-14	54.2	Ring finger domain
`XP_057804591.1`	Pfam	`PF13445`	zf-RING_UBOX	Domain	235	273	4.9e-08	33.6	RING-type zinc-finger
`XP_057804591.1`	Pfam	`PF00097`	zf-C3HC4	Domain	235	275	4.5e-08	33.5	Zinc finger, C3HC4 type (RING finger)

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

2 records

下载 cDNA FASTA

XM_057948608.1
AAAATTATTTGAGGGAGAGAGAGAGGTGGCGTATAAAATATGGTTGATCCGACTAGCCTATTGCCCAATTTCAGAAAAAGCGAAAAAAAGAGCTAAATGCAACGACTGTTTCTCTGTTTTCCCTGCTCTCTGCAAATCAAAAAACTAGTAGTTTTAGAAATCTTTACTCTTTTCGGCCTCAAAATATAGTTTCTTGTTTATCGCTCTCTACACACACTCATTTCACACTTCGGCTTAAGCAATCTTTGTTTCAATTTTAA...

XM_057948607.1
GAGAGAGAGAGGTGGCGTATAAAATATGGTTGATCCGACTAGCCTATTGCCCAATTTCAGAAAAAGCGAAAAAAAGAGCTAAATGCAACGACTGTTTCTCTGTTTTCCCTGCTCTCTGCAAATCAAAAAACTAGTAGTTTTAGAAATCTTTACTCTTTTCGGCCTCAAAATATAGTTTCTTGTTTATCGCTCTCTACACACACTCATTTCACACTTCGGCTTAAGCAATCTTTGTTTCAATTTTAACTGGGCTCGGTTCA...

CDS

2 records

下载 CDS FASTA

LOC131019968
ATGGCGATAAAAGGGGTTGATTTCAAGTGGTATGATGGGTTCTTCTTGTCTATGCTTGCTACAAGTATAATTATTGTGGCAATCAATTGGAAGCGATACCATTCGTGTACACACCCGTTGCACATATGGATTGTGGTTGACTATACGACTGTGTTCATTTTCCGGCTCTTGATGTTTGTGGATAATGGGTTGGCTGCTGGAATGGGATTGGATCTCGGTTGGCAGCAGAGATATAAACGTTTTTATGGAAGGATTGTGGT...

Protein

2 records

下载 Protein FASTA

XP_057804590.1
MAIKGVDFKWYDGFFLSMLATSIIIVAINWKRYHSCTHPLHIWIVVDYTTVFIFRLLMFVDNGLAAGMGLDLGWQQRYKRFYGRIVVLSVLVLLLYPFLLTWTVIGTLWFTRSRNCLPEDGQKWGFLIWLLFSYCGLVCIACICMGKWLTRRQAHLLRAQQGIPVSEYGVLVDMVRVPDWAIEAAGQEMRGMGQDAAMYHPGLYLTPAQVTNELLISEIYLRFHKDNELLISFDIQREAVEALIQELPKFRLKAVPTDCS...

XP_057804591.1
MAIKGVDFKWYDGFFLSMLATSIIIVAINWKRYHSCTHPLHIWIVVDYTTVFIFRLLMFVDNGLAAGMGLDLGWQQRYKRFYGRIVVLSVLVLLLYPFLLTWTVIGTLWFTRSRNCLPEDGQKWGFLIWLLFSYCGLVCIACICMGKWLTRRQAHLLRAQQGIPVSEYGVLVDMVRVPDWAIEAAGQEMRGMGQDAAMYHPGLYLTPAQREAVEALIQELPKFRLKAVPTDCSECPICLEEFRVGNEVRGLPCAHNFHVE...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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