Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr04G002576`

染色体: Chr04 坐标: 41118286-41164029 链方向: + 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr04G002576`
名称	SmilChr04G002576
描述	crossover junction endonuclease EME1B-like
染色体 / SeqID	Chr04 / NC_080390.1
坐标与链方向	41118286 - 41164029 / `+`

ID 对照

NCBI Gene ID	`LOC131021689`
GeneID	`131021689`
locus_tag	`-`
NCBI Name	`LOC131021689`
原始查询	`SmilChr04G002576`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	crossover junction endonuclease EME1B-like
产物	crossover junction endonuclease EME1B-like
Arabidopsis	`AT2G22140` (EME1B) essential meiotic endonuclease 1B [Source:NCBI gene (formerly Entrezgene);Acc:816748] identity=64.6; qcov=0.589; evalue=7.82e-156; confidence=medium
Rice	`Os04g0648700` (EME1) - identity=55.2; qcov=0.606; evalue=9.83e-121; confidence=high
GO	GO:0003677 (DNA binding); GO:0004518 (nuclease activity); GO:0005634 (nucleus); GO:0006281 (DNA repair); GO:0048476 (Holliday junction resolvase complex)
KEGG	smil03440 (Homologous recombination - Salvia miltiorrhiza (redroot sage)); smil03460 (Fanconi anemia pathway - Salvia miltiorrhiza (redroot sage))
Pfam	PF02732 (ERCC4); PF21292 (EME1-MUS81_C)
InterPro	-
eggNOG	-
NR	GeneID:131021689

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Nucleus	细胞核	1.00000	`XP_057806924.1`
2	Chromosome	染色体	0.60000	`XP_057806924.1`
3	Nuclear body	核内小体	0.60000	`XP_057806924.1`
4	Nucleoplasm	核质	0.60000	`XP_057806924.1`
5	PML body	PML body	0.20000	`XP_057806924.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr4:41118390`	`TCC`	`T`	2bp_deletion	frameshift_variant	4	A21,B56,B96,C35
`Chr4:41118390`	`TCC`	`T`	2bp_deletion	CDS_variant	4	A21,B56,B96,C35
`Chr4:41118551`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B44
`Chr4:41118568`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B124,B135,C37
`Chr4:41118620`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B54
`Chr4:41118854`	`G`	`A`	EMS_canonical_SNV	CDS_variant	5	A1,B139,C18,C22,C27
`Chr4:41118928`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B104,B117
`Chr4:41119880`	`G`	`A`	EMS_canonical_SNV	CDS_variant	5	B122,B22,C11,C48,C69
`Chr4:41120790`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	C14,C18,C24
`Chr4:41120990`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B124,B135
`Chr4:41121067`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B108,B27,C23
`Chr4:41121329`	`G`	`A`	EMS_canonical_SNV	CDS_variant	3	B44,B59,C81

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057806924.1

611 aa

306

611

ERCC4

EME1-MUS81_C

PF02732 ERCC4 319-475 aa PF21292 EME1-MUS81_C 516-587 aa

XP_057806925.1

604 aa

302

604

ERCC4

EME1-MUS81_C

PF02732 ERCC4 312-468 aa PF21292 EME1-MUS81_C 509-580 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057806924.1`	Pfam	`PF02732`	ERCC4	Domain	319	475	1.2e-13	51.9	ERCC4 domain
`XP_057806924.1`	Pfam	`PF21292`	EME1-MUS81_C	Domain	516	587	2.9e-10	40.9	EME1/MUS81, C-terminal
`XP_057806925.1`	Pfam	`PF02732`	ERCC4	Domain	312	468	1.2e-13	52.0	ERCC4 domain
`XP_057806925.1`	Pfam	`PF21292`	EME1-MUS81_C	Domain	509	580	2.9e-10	40.9	EME1/MUS81, C-terminal

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

3 records

下载 cDNA FASTA

XR_009101038.1
GCGCAAACAAATTAAATCTAAAACTTGTCACTGTGTTTAGACGACGAAATGTCGCAGCCAATTCGCGTCGACATTCTCTCCGACTCCGACTCCGACTCCGAACCCTGCGGTTATCGACGGAGTCTCAGAGACGAAGCCATTGACCTCACTACGCCGCCGCCATTCCCCCAATCTAAGAAGAAACAGAAAACCCACAACTTTTCAAACCCTAGTAACTCCACCGTCTTCATCATAGACGATGACCACACGCCATTTAAGTC...

XM_057950942.1
GTGATCCCGCGCAAACAAATTAAATCTAAAACTTGTCACTGTGTTTAGACGACGAAATGTCGCAGCCAATTCGCGTCGACATTCTCTCCGACTCCGACTCCGACTCCGAACCCTGCGGTTATCGACGGAGTCTCAGAGACGAAGCCATTGACCTCACTACGCCGCCGCCATTCCCCCAATCTAAGAAGAAACAGAAAACCCACAACTTTTCAAACCCTAGTAACTCCACCGTCTTCATCATAGACGATGACCACACGCCA...

CDS

2 records

下载 CDS FASTA

LOC131021689
ATGTCGCAGCCAATTCGCGTCGACATTCTCTCCGACTCCGACTCCGACTCCGAACCCTGCGGTTATCGACGGAGTCTCAGAGACGAAGCCATTGACCTCACTACGCCGCCGCCATTCCCCCAATCTAAGAAGAAACAGAAAACCCACAACTTTTCAAACCCTAGTAACTCCACCGTCTTCATCATAGACGATGACCACACGCCATTTAAGTCAAATAGGCAATCCTCTCCCACTCCTTCGCCATTCTCCGATGCGTCCCT...

Protein

2 records

下载 Protein FASTA

XP_057806924.1
MSQPIRVDILSDSDSDSEPCGYRRSLRDEAIDLTTPPPFPQSKKKQKTHNFSNPSNSTVFIIDDDHTPFKSNRQSSPTPSPFSDASLVKCSRGTSSFSVASCFLAEGLATEQLVVAETPISELLKSEVAISKCNTRISTAEDARPFITNHNSGEIKGWICVESDDESEDLGEIGALKDNPSTFAAKLADETEFPPRLVESSSFSCATSFQLQRKATQNPVRTHILEGCSTPGTSEDGISEVNNTCFLKEKKKRDETVKLK...

XP_057806925.1
MSQPIRVDILSDSDSDSEPCGYRRSLRDEAIDLTTPPPFPQSKKKQKTHNFSNPSNSTVFIIDDDHTPFKSNRQSSPTPSPFSDASLVKCSRGTSSFSEGLATEQLVVAETPISELLKSEVAISKCNTRISTAEDARPFITNHNSGEIKGWICVESDDESEDLGEIGALKDNPSTFAAKLADETEFPPRLVESSSFSCATSFQLQRKATQNPVRTHILEGCSTPGTSEDGISEVNNTCFLKEKKKRDETVKLKRTAKEER...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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