Gene Card

Search by standard ID, NCBI Gene ID, GeneID, locus tag, transcript ID, protein ID, or a manually reviewed literature gene name. The page resolves the query to a Red Sage gene and integrates coordinates, sequences, annotations, subcellular localization, EMS variants, and downloads.

Resolved gene

`SmilChr04G003357`

Chromosome: Chr04 Coordinates: 50167766-50173752 Strand: - Biotype: protein_coding

Basic Information

Standard Gene ID	`SmilChr04G003357`
Name	SmilChr04G003357
Description	kinesin-like protein NACK1
Chromosome / SeqID	Chr04 / NC_080390.1
Coordinates and Strand	50167766 - 50173752 / `-`

ID Mapping

NCBI Gene ID	`LOC131020253`
GeneID	`131020253`
locus_tag	`-`
NCBI Name	`LOC131020253`
Original query	`SmilChr04G003357`

Open ID mapping

Functional Annotation

Source	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
Description	kinesin-like protein NACK1
Product	kinesin-like protein NACK1
Arabidopsis	`AT1G18370` (HIK) ATP binding microtubule motor family protein [Source:NCBI gene (formerly Entrezgene);Acc:838418] identity=73.7; qcov=1; evalue=0; confidence=high
Rice	`Os01g0513900` (OsACK1) - identity=65.2; qcov=1; evalue=0; confidence=high
GO	GO:0003777 (microtubule motor activity); GO:0005524 (ATP binding); GO:0007018 (microtubule-based movement); GO:0008017 (microtubule binding)
KEGG	smil04814 (Motor proteins - Salvia miltiorrhiza (redroot sage))
Pfam	PF00225 (Kinesin); PF11995 (DUF3490); PF16796 (Microtub_bd)
InterPro	-
eggNOG	-
NR	GeneID:131020253

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Subcellular Localization Top 5

Rank	Location	Chinese	Score	Protein ID
1	Centrosome	中心体	1.00000	`XP_057804943.1`
2	Cytoskeleton	细胞骨架	1.00000	`XP_057804943.1`
3	Spindle	Spindle	1.00000	`XP_057804943.1`
4	Spindle pole	Spindle pole	1.00000	`XP_057804943.1`
5	Midbody	Midbody	1.00000	`XP_057804943.1`

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EMS Mutant Variants

The table shows the first 12 candidate EMS variants for this gene. Open the EMS module for full filtering and downloads.

Position	REF	ALT	Type	Effect	Carriers	Samples
`Chr4:50168440`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B28
`Chr4:50168603`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	D11
`Chr4:50169312`	`CT`	`C`	1bp_deletion	frameshift_variant	105	A10,A11,A13,A18,A19,A22,A23,A3,A5,A7,A8,B10,B101,B102,B103,B106,B107,B11,B112,B113,B114,B12,B120,B122,B124,B129,B13,B131,B135,B138,B139,B18,B21,B22,B25,B26,B29,B31,B34,B37,B38,B39,B41,B44,B45,B46,B47,B48,B51,B53,B54,B59,B6,B60,B65,B66,B68,B7,B70,B72,B75,B76,B8,B82,B84,B85,B86,B90,B92,B93,B94,C10,C14,C15,C16,C20,C26,C31,C34,C35,C38,C43,C47,C48,C50,C58,C59,C6,C60,C63,C67,C71,C73,C74,C76,C79,C80,C81,C87,C9,D10,D11,D4,D7,D9
`Chr4:50169607`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B56,B96
`Chr4:50169626`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B132,B3,B61
`Chr4:50170118`	`GTC`	`G`	2bp_deletion	frameshift_variant	1	B28
`Chr4:50170118`	`GTC`	`G`	2bp_deletion	CDS_variant	1	B28
`Chr4:50170828`	`G`	`A`	EMS_canonical_SNV	CDS_variant	5	B137,B28,B30,B34,C49
`Chr4:50171149`	`G`	`A`	EMS_canonical_SNV	CDS_variant	5	B137,B28,B30,B34,C49
`Chr4:50171854`	`C`	`T`	EMS_canonical_SNV	CDS_variant	5	B137,B28,B30,B34,C49
`Chr4:50172302`	`C`	`T`	EMS_canonical_SNV	CDS_variant	5	B137,B28,B30,B34,C49
`Chr4:50172358`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	D11

Open EMS variants

Predicted Protein Domains

Domain results are from the Pfam-A HMM database and HMMER hmmscan; positions are amino-acid coordinates on the corresponding protein sequence.

XP_057804943.1

970 aa

485

970

Microtub_bd

Kinesin

DUF3490

PF16796 Microtub_bd 36-170 aa PF00225 Kinesin 39-355 aa PF11995 DUF3490 783-945 aa

Protein ID	Source	Domain ID	Domain name	Type	Start aa	End aa	E-value	Score	Description
`XP_057804943.1`	Pfam	`PF16796`	Microtub_bd	Domain	36	170	3.4e-21	76.5	Microtubule binding
`XP_057804943.1`	Pfam	`PF00225`	Kinesin	Domain	39	355	1e-100	337.7	Kinesin motor domain
`XP_057804943.1`	Pfam	`PF11995`	DUF3490	Family	783	945	1.6e-66	223.7	Domain of unknown function (DUF3490)

cDNA / CDS / Protein Sequences

Download genomic DNA (gene region only)

Every public gene has transcript sequence from the reference annotation. Protein-coding genes also have CDS and protein sequences. CDS and protein downloads do not apply to noncoding RNA. Version suffixes of original accessions are retained.

cDNA

1 records

Download cDNA FASTA

XM_057948960.1
GGTACCCGAGAAATAATTTCGGATTGGGTCGAATTTTCGGGTAGGGTACTCGACCCGTACCGAGTATCCAGCCGCTCACTTTATGACAGCGAGACATGAGATTTAAAATAGCCGTTGGCACATTAGAAGTAGCCGTTGCACGTTTTGAACTCAAAATCCAATCAACCCTCCTCACATACAATTGACTCCTCTCTCTCCACTTTCCCCCCATCTCTCTCTTTTGCTCCCATATTGCTTTCCATTTTGATTTGTTTATTTCT...

CDS

1 records

Download CDS FASTA

LOC131020253
ATGACTGTTAGAACACCAGGCACACCAGCCTCAAAAGTGGACAGGACACCAGCAGCTGCCACTCCAGGTGGAAATACAAATAGAGCAAGGGAAGAGAAAATTGTTGTAACTGTAAGACTAAGGCCTCTGAGCAAGAGAGAGCAAGCATCTAAAGATCAGATAGCCTGGGAATGCATTGATGATCATACAATTGTCTATAATCCGAGCCCCCAAGAACGTGCAGTTCTACCTGCATCTTTTTCATTCGATAAAGTGTTCGG...

Protein

1 records

Download Protein FASTA

XP_057804943.1
MTVRTPGTPASKVDRTPAAATPGGNTNRAREEKIVVTVRLRPLSKREQASKDQIAWECIDDHTIVYNPSPQERAVLPASFSFDKVFGPDCSTENVYEDGVKTVALSALMGINATIFAYGQTSSGKTYTMRGITEKAVNDIYMHIINTPERDFRIKISGLEIYNENVRDLLNSESGRSLKLLDDPEKGTVVEKLVEETAKDGQHLRHLIHVCDAQRQVGETALNDTSSRSHQIIRLTIESTLRESSNCVRSYVASLNFVDL...

Flanking Sequence Downloads

Upstream/downstream length is capped at 10000 bp. Negative-strand genes are reverse-complemented so the exported FASTA follows transcriptional 5′ to 3′ orientation.

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