Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr04G003363`

染色体: Chr04 坐标: 50212442-50219328 链方向: - 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr04G003363`
名称	SmilChr04G003363
描述	DNA mismatch repair protein MSH2
染色体 / SeqID	Chr04 / NC_080390.1
坐标与链方向	50212442 - 50219328 / `-`

ID 对照

NCBI Gene ID	`LOC131020260`
GeneID	`131020260`
locus_tag	`-`
NCBI Name	`LOC131020260`
原始查询	`SmilChr04G003363`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	DNA mismatch repair protein MSH2
产物	DNA mismatch repair protein MSH2
Arabidopsis	`AT3G18524` (MSH2) MUTS homolog 2 [Source:NCBI gene (formerly Entrezgene);Acc:821383] identity=72.2; qcov=1; evalue=0; confidence=high
Rice	`Os05g0274200` - identity=68.7; qcov=0.999; evalue=0; confidence=high
GO	GO:0005524 (ATP binding); GO:0005634 (nucleus); GO:0006298 (mismatch repair); GO:0006312 (mitotic recombination); GO:0030983 (mismatched DNA binding); GO:0032301 (MutSalpha complex); GO:0140664 (ATP-dependent DNA damage sensor activity)
KEGG	smil03430 (Mismatch repair - Salvia miltiorrhiza (redroot sage))
Pfam	PF00488 (MutS_V); PF01624 (MutS_I); PF05188 (MutS_II); PF05190 (MutS_IV); PF05192 (MutS_III)
InterPro	-
eggNOG	-
NR	GeneID:131020260

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Nucleus	细胞核	1.00000	`XP_057804951.1`
2	Chromosome	染色体	0.40000	`XP_057804951.1`
3	Nucleoplasm	核质	0.40000	`XP_057804951.1`
4	Cytoplasm	细胞质	0.40000	`XP_057804951.1`
5	Centromere	着丝粒	0.00000	`XP_057804951.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr4:50212712`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C50
`Chr4:50212958`	`G`	`A`	EMS_canonical_SNV	CDS_variant	5	B33,B4,B89,C19,C86
`Chr4:50213031`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C40
`Chr4:50213302`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	D7
`Chr4:50213454`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C8
`Chr4:50214473`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C80
`Chr4:50214502`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C50
`Chr4:50214672`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C57
`Chr4:50214708`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C50
`Chr4:50214740`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C40
`Chr4:50215589`	`AT`	`A`	1bp_deletion	frameshift_variant	179	A1,A11,A14,A15,A16,A18,A19,A2,A20,A21,A22,A23,A24,A25,A3,A4,A5,A6,A8,A9,B1,B10,B102,B103,B104,B105,B106,B107,B108,B109,B11,B111,B112,B113,B114,B115,B117,B118,B119,B12,B122,B123,B127,B132,B133,B134,B136,B137,B139,B14,B140,B19,B2,B20,B21,B22,B23,B25,B26,B27,B28,B30,B31,B33,B36,B37,B40,B41,B42,B43,B45,B46,B47,B48,B49,B5,B50,B51,B53,B54,B55,B58,B59,B6,B60,B67,B68,B69,B70,B71,B72,B73,B74,B76,B77,B78,B79,B8,B80,B82,B83,B84,B85,B86,B88,B9,B93,B94,B95,B96,B97,B98,B99,C1,C15,C16,C19,C2,C20,C21,C27,C29,C3,C30,C31,C32,C35,C36,C38,C39,C4,C40,C41,C42,C44,C45,C46,C49,C51,C52,C53,C54,C56,C57,C59,C6,C60,C61,C62,C63,C65,C68,C7,C70,C71,C72,C74,C75,C77,C78,C79,C8,C81,C82,C83,C84,C86,C87,C9,D1,D10,D11,D3,D4,D5,D6,D7,D8,D9
`Chr4:50215750`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C23

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057804951.1

946 aa

473

946

MutS_I

MutS_II

MutS_III

MutS_IV

MutS_V

PF01624 MutS_I 23-128 aa PF05188 MutS_II 144-283 aa PF05192 MutS_III 299-611 aa PF05190 MutS_IV 468-565 aa PF00488 MutS_V 667-866 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057804951.1`	Pfam	`PF01624`	MutS_I	Domain	23	128	5.9e-17	62.6	MutS domain I
`XP_057804951.1`	Pfam	`PF05188`	MutS_II	Domain	144	283	8.7e-17	62.5	MutS domain II
`XP_057804951.1`	Pfam	`PF05192`	MutS_III	Domain	299	611	2.1e-32	113.7	MutS domain III
`XP_057804951.1`	Pfam	`PF05190`	MutS_IV	Domain	468	565	1.6e-19	70.7	MutS family domain IV
`XP_057804951.1`	Pfam	`PF00488`	MutS_V	Domain	667	866	1.7e-77	260.4	MutS domain V

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

1 records

下载 cDNA FASTA

XM_057948968.1
AAAAAGGGCGCATCAGGCATTAATAGGGAAAGTGTGAGCACGCGAGGCGCCCAGCTCCAACTTATTCCCGCCAATCCCAAACCTTAAAACAATGGCGTTTCCACTTCACAGTCTTCACTCTCGTAAACACACGACCAAATTTCCCAAGAGTTTCCTATTGTAATTTGTGATCAACAGAAGAAATAGCAATCTCACACCGCATTGCTGCTTCAAATTGTAAGTTCAGCTATCCTGCTCAGGATAATGGCCGACAATTTTGA...

CDS

1 records

下载 CDS FASTA

LOC131020260
ATGGCCGACAATTTTGAGGACCACAACAAGCTTCCCGAACTCAAACTCGATGCCAAACAAGCTCAAGGGTTTCTCTCATTCTTCAAAAAGCTACACACTGACCCACGAGCCATTCGATTCTTCGATCGCAAGGACTACTATACTACGCATGGCGAGAATGCAACCTTCATTGCCAAGACCTATTACCACACTACAACTGCTGTGCGCCAACTAGGAAGTGGCTCGGATGCCATTTCTAGTGTTAGCGTAAGCAAGAATAT...

Protein

1 records

下载 Protein FASTA

XP_057804951.1
MADNFEDHNKLPELKLDAKQAQGFLSFFKKLHTDPRAIRFFDRKDYYTTHGENATFIAKTYYHTTTAVRQLGSGSDAISSVSVSKNMFETVARDLLLERTDHTLELYEGNGANWRLVKSGSPGNLGSFEEILFANNEMQDSPAIVAVISNLRESGCTVGLGYVDLTKRVLGLAEFPDDSHYTNLESTLVALGCKECLLPADIAKSSEFKSLTDALSRCGVMVMERKKGEFKGRDLVQDLGRLVKGSIDPVRDLLAAFEFA...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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