当前解析结果
SmilChr04G004114| 统一新 Gene ID | SmilChr04G004114 |
|---|---|
| 名称 | SmilChr04G004114 |
| 描述 | glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a |
| 染色体 / SeqID | Chr04 / NC_080390.1 |
| 坐标与链方向 | 56046703 - 56051936 / - |
| NCBI Gene ID | LOC131020988 |
|---|---|
| GeneID | 131020988 |
| locus_tag | - |
| NCBI Name | LOC131020988 |
| 原始查询 | SmilChr04G004114 |
| 来源 | GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog |
|---|---|
| 描述 | glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a |
| 产物 | glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a |
| Arabidopsis | AT3G06860 (MFP2)multifunctional protein 2 [Source:NCBI gene (formerly Entrezgene);Acc:819870] identity=74.1; qcov=0.999; evalue=0; confidence=high |
| Rice | Os01g0348600- identity=74.7; qcov=0.993; evalue=0; confidence=high |
| GO | GO:0003857 ((3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity); GO:0005777 (peroxisome); GO:0006635 (fatty acid beta-oxidation); GO:0070403 (NAD+ binding) |
| KEGG | smil00071 (Fatty acid degradation - Salvia miltiorrhiza (redroot sage)); smil00592 (alpha-Linolenic acid metabolism - Salvia miltiorrhiza (redroot sage)); smil01100 (Metabolic pathways - Salvia miltiorrhiza (redroot sage)); smil01110 (Biosynthesis of secondary metabolites - Salvia miltiorrhiza (redroot sage)); smil01212 (Fatty acid metabolism - Salvia miltiorrhiza (redroot sage)) |
| Pfam | PF00378 (ECH_1); PF00725 (3HCDH); PF02737 (3HCDH_N); PF16113 (ECH_2) |
| InterPro | - |
| eggNOG | - |
| NR | GeneID:131020988 |
| Rank | 定位位置 | 中文 | Score | Protein ID |
|---|---|---|---|---|
| 1 | Cytosol | 细胞质基质 | 1.00000 | XP_057806039.1 |
| 2 | Peroxisome | 过氧化物酶体 | 1.00000 | XP_057806041.1 |
| 3 | Chloroplast | 叶绿体 | 0.20000 | XP_057806039.1 |
| 4 | Mitochondrion | 线粒体 | 0.20000 | XP_057806041.1 |
| 5 | Nucleus | 细胞核 | 0.00000 | XP_057806039.1 |
下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。
| 位置 | REF | ALT | 类型 | 影响 | 携带材料数 | 材料 |
|---|---|---|---|---|---|---|
Chr4:56046876 |
G |
A |
EMS_canonical_SNV | CDS_variant | 1 | C75 |
Chr4:56046918 |
C |
T |
EMS_canonical_SNV | CDS_variant | 2 | D10,D11 |
Chr4:56046972 |
G |
A |
EMS_canonical_SNV | stop_gained | 7 | B129,B14,B38,B39,C38,D10,D11 |
Chr4:56046977 |
C |
CT |
1bp_insertion | frameshift_variant | 183 | A1,A10,A13,A15,A18,A2,A20,A21,A22,A23,A25,A3,A5,A6,A7,A8,B1,B10,B102,B107,B110,B111,B112,B113,B114,B115,B119,B12,B121,B122,B123,B124,B128,B13,B131,B132,B134,B135,B137,B138,B140,B15,B17,B18,B2,B20,B24,B25,B26,B27,B28,B29,B3,B30,B31,B37,B4,B40,B41,B42,B43,B45,B46,B47,B48,B49,B50,B51,B52,B53,B54,B56,B57,B59,B60,B61,B62,B64,B65,B66,B67,B68,B69,B7,B70,B71,B72,B73,B74,B75,B77,B78,B79,B8,B80,B81,B82,B83,B84,B85,B86,B88,B89,B90,B91,B92,B94,B95,B96,B99,C1,C10,C11,C12,C13,C14,C15,C18,C20,C21,C22,C23,C24,C25,C26,C27,C28,C29,C3,C30,C31,C32,C33,C35,C36,C37,C38,C39,C4,C40,C41,C42,C43,C44,C45,C48,C51,C53,C54,C56,C59,C6,C61,C62,C63,C64,C66,C67,C68,C69,C71,C72,C73,C75,C76,C78,C79,C8,C80,C83,C84,C85,C86,D10,D11,D2,D3,D4,D5,D6,D7,D8,D9 |
Chr4:56046990 |
C |
T |
EMS_canonical_SNV | CDS_variant | 1 | B10 |
Chr4:56046998 |
C |
CGG |
2bp_insertion | frameshift_variant | 2 | D10,D11 |
Chr4:56046998 |
C |
CGG |
2bp_insertion | CDS_variant | 2 | D10,D11 |
Chr4:56047015 |
C |
T |
EMS_canonical_SNV | CDS_variant | 3 | C24,C76,D8 |
Chr4:56047017 |
C |
T |
EMS_canonical_SNV | CDS_variant | 1 | B10 |
Chr4:56047036 |
G |
A |
EMS_canonical_SNV | CDS_variant | 4 | A19,B13,D10,D11 |
Chr4:56047064 |
C |
T |
EMS_canonical_SNV | CDS_variant | 4 | A8,B128,C37,D8 |
Chr4:56047070 |
T |
TAC |
2bp_insertion | frameshift_variant | 29 | A10,A13,A2,A21,B110,B111,B12,B138,B2,B20,B24,B27,B29,B51,B59,B71,B74,B82,B95,C27,C28,C3,C31,C33,C43,C44,C45,C54,C67 |
结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。
XP_057806039.1PF00378
ECH_1 15-205 aa
PF16113
ECH_2 24-192 aa
PF02737
3HCDH_N 313-491 aa
PF00725
3HCDH 494-587 aa
XP_057806041.1PF00378
ECH_1 15-205 aa
PF16113
ECH_2 24-192 aa
PF02737
3HCDH_N 313-491 aa
PF00725
3HCDH 494-587 aa
| Protein ID | 来源 | 结构域编号 | 结构域名称 | 类型 | 起始 aa | 终止 aa | E-value | Score | 说明 |
|---|---|---|---|---|---|---|---|---|---|
XP_057806039.1 |
Pfam | PF00378 |
ECH_1 | Domain | 15 | 205 | 1.2e-38 | 133.6 | Enoyl-CoA hydratase/isomerase |
XP_057806039.1 |
Pfam | PF16113 |
ECH_2 | Domain | 24 | 192 | 3.1e-26 | 93.4 | Enoyl-CoA hydratase/isomerase |
XP_057806039.1 |
Pfam | PF02737 |
3HCDH_N | Domain | 313 | 491 | 4.9e-61 | 206.5 | 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain |
XP_057806039.1 |
Pfam | PF00725 |
3HCDH | Domain | 494 | 587 | 3.9e-25 | 88.9 | 3-hydroxyacyl-CoA dehydrogenase, C-terminal domain |
XP_057806041.1 |
Pfam | PF00378 |
ECH_1 | Domain | 15 | 205 | 1.1e-38 | 133.8 | Enoyl-CoA hydratase/isomerase |
XP_057806041.1 |
Pfam | PF16113 |
ECH_2 | Domain | 24 | 192 | 2.6e-26 | 93.6 | Enoyl-CoA hydratase/isomerase |
XP_057806041.1 |
Pfam | PF02737 |
3HCDH_N | Domain | 313 | 491 | 4.3e-61 | 206.7 | 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain |
XP_057806041.1 |
Pfam | PF00725 |
3HCDH | Domain | 494 | 587 | 2.8e-25 | 89.4 | 3-hydroxyacyl-CoA dehydrogenase, C-terminal domain |
每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。
2 records
下载 cDNA FASTA2 records
下载 CDS FASTA2 records
下载 Protein FASTAupstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。