Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr05G000049`

染色体: Chr05 坐标: 229556-230703 链方向: + 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr05G000049`
名称	SmilChr05G000049
描述	metalloendoproteinase 5-MMP-like
染色体 / SeqID	Chr05 / NC_080391.1
坐标与链方向	229556 - 230703 / `+`

ID 对照

NCBI Gene ID	`LOC131026535`
GeneID	`131026535`
locus_tag	`-`
NCBI Name	`LOC131026535`
原始查询	`SmilChr05G000049`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	metalloendoproteinase 5-MMP-like
产物	metalloendoproteinase 5-MMP-like
Arabidopsis	`AT1G59970` (AT1G59970) Matrixin family protein [Source:NCBI gene (formerly Entrezgene);Acc:842291] identity=44.4; qcov=0.962; evalue=2.64e-73; confidence=medium
Rice	`Os02g0740700` (OsMMP1) - identity=44.2; qcov=0.952; evalue=1.1e-69; confidence=medium
GO	GO:0004222 (metalloendopeptidase activity); GO:0006508 (proteolysis); GO:0008270 (zinc ion binding); GO:0030198 (extracellular matrix organization); GO:0030574 (collagen catabolic process); GO:0031012 (extracellular matrix)
KEGG	-
Pfam	PF00413 (Peptidase_M10); PF01471 (PG_binding_1)
InterPro	-
eggNOG	-
NR	GeneID:131026535

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Secreted	分泌到胞外	0.80000	`XP_057812404.1`
2	Vacuole	液泡	0.40000	`XP_057812404.1`
3	Cell wall	细胞壁	0.20000	`XP_057812404.1`
4	Nucleus	细胞核	0.00000	`XP_057812404.1`
5	Chromosome	染色体	0.00000	`XP_057812404.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr5:229741`	`T`	`TGA`	2bp_insertion	frameshift_variant	1	B49
`Chr5:229741`	`T`	`TGA`	2bp_insertion	CDS_variant	1	B49
`Chr5:229747`	`ATT`	`A`	2bp_deletion	frameshift_variant	7	A5,B112,B2,C15,C34,C43,C58
`Chr5:229855`	`CA`	`C`	1bp_deletion	frameshift_variant	9	A11,A2,B18,B49,B73,C39,C41,C67,C8
`Chr5:229898`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	A14,B62
`Chr5:229919`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	C11,C69
`Chr5:229939`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C80
`Chr5:230159`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B48,C11,C69
`Chr5:230164`	`AC`	`A`	1bp_deletion	frameshift_variant	3	B49,C41,D11
`Chr5:230164`	`AC`	`A`	1bp_deletion	CDS_variant	3	B49,C41,D11
`Chr5:230209`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C16
`Chr5:230225`	`C`	`T`	EMS_canonical_SNV	CDS_variant	5	A11,B18,B48,C39,C8

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057812404.1

314 aa

157

314

PG_binding_1

Peptidase_M10

PF01471 PG_binding_1 59-117 aa PF00413 Peptidase_M10 164-312 aa

XP_057812405.1

290 aa

145

290

PG_binding_1

Peptidase_M10

PF01471 PG_binding_1 59-117 aa PF00413 Peptidase_M10 140-288 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057812404.1`	Pfam	`PF01471`	PG_binding_1	Domain	59	117	4.7e-13	49.8	Putative peptidoglycan binding domain
`XP_057812404.1`	Pfam	`PF00413`	Peptidase_M10	Domain	164	312	2.1e-41	142.1	Matrixin
`XP_057812405.1`	Pfam	`PF01471`	PG_binding_1	Domain	59	117	3.9e-13	50.1	Putative peptidoglycan binding domain
`XP_057812405.1`	Pfam	`PF00413`	Peptidase_M10	Domain	140	288	1.7e-41	142.5	Matrixin

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

2 records

下载 cDNA FASTA

XM_057956422.1
TATTGCATTAACATGCATCACAAAACAAATTAAATATTCTTAAGAGTTTTTCAAGTATTCATTCATGGCTTTTAAGATTTTTCCTCTCATCTCCTTTCTCTTCCTCATTTTCATACTACTTGCTCCTAGTGGCCATGCCAAGAGGAGCATCCCAAGTGGCAAAAAAACCTTTTTTGCCTCAGCTTTTGAATATTTCATCAAGAAATTAAAAGGTTGTCACAAGGGAAACACTACTAAAGGGATCCATGAGCTCAAAGCCT...

XM_057956421.1
TTGCATTAACATGCATCACAAAACAAATTAAATATTCTTAAGAGTTTTTCAAGTATTCATTCATGGCTTTTAAGATTTTTCCTCTCATCTCCTTTCTCTTCCTCATTTTCATACTACTTGCTCCTAGTGGCCATGCCAAGAGGAGCATCCCAAGTGGCAAAAAAACCTTTTTTGCCTCAGCTTTTGAATATTTCATCAAGAAATTAAAAGGTTGTCACAAGGGAAACACTACTAAAGGGATCCATGAGCTCAAAGCCTAC...

CDS

2 records

下载 CDS FASTA

LOC131026535
ATGGCTTTTAAGATTTTTCCTCTCATCTCCTTTCTCTTCCTCATTTTCATACTACTTGCTCCTAGTGGCCATGCCAAGAGGAGCATCCCAAGTGGCAAAAAAACCTTTTTTGCCTCAGCTTTTGAATATTTCATCAAGAAATTAAAAGGTTGTCACAAGGGAAACACTACTAAAGGGATCCATGAGCTCAAAGCCTACCTCGAGAAATTCGGTTATCTCAGATATCCAAACAAAACACAAGCCACTGATGATGATTTCGA...

Protein

2 records

下载 Protein FASTA

XP_057812404.1
MAFKIFPLISFLFLIFILLAPSGHAKRSIPSGKKTFFASAFEYFIKKLKGCHKGNTTKGIHELKAYLEKFGYLRYPNKTQATDDDFDDILESAVKTYQENYHIKPSGIVDDETISKMTTPRCGVPDIINGTNYMHPRHRNHKSSGSSGVHVVSNYSFFDGEPKWPPSKTHLTYGFLPNFPANAVAPVARAFQKWQSSTHFTFARAQTNADLVIGFYRGDHGDGAPFDGPLGDSIAHAFAPPDGRFHYDADETWSIGAVAG...

XP_057812405.1
MAFKIFPLISFLFLIFILLAPSGHAKRSIPSGKKTFFASAFEYFIKKLKGCHKGNTTKGIHELKAYLEKFGYLRYPNKTQATDDDFDDILESAVKTYQENYHIKPSGIVDDETISKMTTPRCGVPDIINGYSFFDGEPKWPPSKTHLTYGFLPNFPANAVAPVARAFQKWQSSTHFTFARAQTNADLVIGFYRGDHGDGAPFDGPLGDSIAHAFAPPDGRFHYDADETWSIGAVAGAFDLETVALHEIGHLLGLGHSMDE...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

下载 upstream 下载 downstream 下载 upstream + gene + downstream

JBrowse

跳转到基因组浏览器